Method for doubling chromosome complement of maize haploid plant

Abstract

The invention discloses a method for doubling a chromosome complement of a maize haploid plant. The method comprises the following steps: firstly, preparing a colchicine solution, adding an infiltration aid dimethyl sulfoxide, and mixing uniformly to obtain an injection liquid; and injecting 80-100 microliters of the injection liquid into a growth point of a stem tip of the haploid maize plant, carrying out routine nursing on the maize plant receiving the injection, and thus harvesting a chromosome complement doubled seed. The method can greatly improve the chromosome complement doubling success rate, reduces the workload of scientific research personnel, and shortens the seed-culturing time; and the scientific research personnel do not need to be exposed in a colchicine polluted environment for a long time, and body health and safety are ensured.

Description

technical field [0001] The invention belongs to the field of agricultural breeding, and in particular relates to a method for doubling the genome of maize haploid plants. Background technique [0002] In traditional maize breeding, doubled haploid (DH) technology is often used to induce haploids using parthenogenetic induction lines, and then double to form diploids. Generally, 100 haploids can be obtained after only two generations. % of the homozygous line, which is called DH (Doubled Haploid) line, the breeding process is faster. [0003] Because maize haploid plants only contain one set of chromosomes, their growth vigor is generally weak, and most of them show high sterility, so the direct application efficiency in production is extremely low. Only by doubling the haploid and making the plants loose powder to obtain the DH line can it be used in large-scale breeding. The limitation of maize haploid doubling technology is the main limiting factor for the large-scale ap...

AI Technical Summary

Problems solved by technology

The chemical agent method is divided into soaking method and titration method in actual use: the soaking method generally uses 0.06% to 0.5% colchicine solution to soak corn seeds, sprouts or roots of seedlings for 12 to 48 hours. After the materials are cleaned, they are transferred to the greenhouse for cultivation or planted in the field. The doubling rate can reach 20-50%. This method is widely used because of its high doubling efficiency, but this method requires the use of colchicine solution for a long time soaking corn materials, and then It also needs to be cleaned and transplanted. The highly toxic carcinogen-colchicine is in an open environment for a long time. During the process of soaking, cleaning and transplanting, scientific researchers have to be exposed to colchicine for a long time, which threatens their health and safety; the titration method Use a dropper to drop 0.06% to 0.5% colchicine solution on the growth points of cotyledons and seedlings. Since the solution drips and evaporates, the drug effect will decrease, so it needs to be titrated every 6 to 8 hours, and repeated for several days. This method requires researchers to repeatedly titrate regularly, the procedure is cumbersome, and the workload is heavy. In addition, the researchers are exposed to colchicine open environment during repeated titration operations, which will damage their health.

Images