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A kind of yoghurt containing bifidobacteria embedding beads or embedding block and its preparation method

A bifidobacterium and bifidobacterium longum technology, applied in dairy products, bacteria, milk preparations, etc., can solve the problems of short shelf life, low activity of bifidobacteria, and difficult storage, etc., to achieve a simple method and realize the probiotic effect , the effect of increasing the number of live bacteria

Active Publication Date: 2015-08-19
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Another object of the present invention is to provide a method for preparing bifidobacterium-embedded yogurt beads or blocks, which solves the problems of low activity of bifidobacteria, short shelf life and difficult storage in bifidobacterium-embedded yogurt products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Preparation of bifidobacterium-embedded beads

[0045] A. Bacteria activation test tube culture

[0046] The medium consists of 15g peptone, 10g beef extract, 3g tryptone, 1g yeast extract powder, 15g glucose, 5g dipotassium hydrogen phosphate, 0.5g sodium acetate, 0.5g diammonium hydrogen citrate, 0.1 g zinc sulfate heptahydrate, Magnesium sulfate heptahydrate 0.05g, L-cysteine ​​hydrochloride 0.1g, fructooligosaccharide 0.5g, calcium carbonate 0.5g, Tween-80 5g and water 1000g. The pH value of the medium is 7.0, and it is used after being sterilized by moist heat at 0.05MpaMpa for 30 minutes;

[0047] Before the strain activation, Bifidobacterium longum subsp. longum ( Bifidobacterium longum subsp. longum ) ampoule bottle at room temperature for 2 hours, then wipe the surface of the ampoule bottle with a 75% alcohol cotton ball in a sterile room for disinfection, open the ampoule bottle, draw 0.2 mL of medium into the ampoule bottle with a sterile pipette, and p...

Embodiment 2

[0067] (1) Preparation of bifidobacteria embedding block

[0068] A. Bacteria activation test tube culture

[0069] The medium consists of 15g of peptone, 10g of beef extract, 3g of tryptone, 1g of yeast extract powder, 15g of glucose, 5g of dipotassium hydrogen phosphate, 0.5g of sodium acetate, 0.5g of diammonium hydrogen citrate, 0.1g of zinc sulfate heptahydrate, Magnesium sulfate heptahydrate 0.05g, L-cysteine ​​hydrochloride 0.1g, fructooligosaccharides 0.5g, calcium carbonate 0.5g, Tween-80 5g and water 1000g; the pH value of the medium is 7.5, at 0.14Mpa Use after 15 minutes of moist heat sterilization;

[0070] Before the strain activation, Bifidobacterium longum subsp. infantis ( Bifidobacterium longum subsp. infantis ) ampoule bottle at room temperature for 3 hours, then wipe the surface of the ampoule bottle with a 75% alcohol cotton ball in a sterile room for disinfection, open the ampoule bottle, draw 0.25mL of medium into the ampoule bottle with a sterile pip...

Embodiment 3

[0091] (1) Preparation of bifidobacterium-embedded beads

[0092] A. Bacteria activation test tube culture

[0093] The medium consists of 15g peptone, 10g beef extract, 3g tryptone, 1g yeast extract powder, 15g glucose, 5g dipotassium hydrogen phosphate, 0.5g sodium acetate, 0.5g diammonium hydrogen citrate, 0.1 g zinc sulfate heptahydrate, Magnesium sulfate heptahydrate 0.05g, L-cysteine ​​hydrochloride 0.1g, fructooligosaccharide 0.5g, calcium carbonate 0.5g, Tween-80 5g and water 1000g. The pH value of the medium is 6.0, and it is used after being sterilized by moist heat at 0.10Mpa for 20 minutes;

[0094] Before the strain activation, Bifidobacterium adolescent ( Bifidobacterium adolescentis ) ampoule bottle at room temperature for 2.5 h, then wipe the surface of the ampoule bottle with a 75% alcohol cotton ball in a sterile room for disinfection, open the ampoule bottle, draw 0.3 mL of medium into the ampoule bottle with a sterile pipette, and put The bacteria in th...

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Abstract

The invention discloses yogurt containing bifidobacterium embedding beads or embedding blocks and a preparation method thereof and aims to solve the problems that the bifidobacterium in a bifidobacterium drink product is low in activity, short in storage period and difficult to store. The yogurt contains bifidobacterium embedding beads or embedding blocks, and the preparation method is divided into two links, namely preparation of the bifidobacterium embedding beads or embedding blocks, and preparation of the yogurt containing the bifidobacterium embedding beads or embedding blocks. The problems that the bifidobacterium is low in viable count in the yogurt content, the storage period is short, and the bifidobacterium is hard to reach the intestinal tract in batches after being eaten are effectively solved, the viable count of the bifidobacterium, the activity of the bifidobacterium and the storage period of the bifidobacterium are effectively guaranteed, and a probiotic role of the bifidobacterium to a human body is realized. The yogurt containing bifidobacterium embedding beads or embedding blocks is mild in preparation conditions, and the method is simple and convenient and is convenient for industrial production.

Description

technical field [0001] The invention relates to yoghurt with bifidobacteria embedded beads or embedded blocks and a preparation method thereof. Background technique [0002] Bifidobacterium is a normal physiological microorganism in the human body. It colonizes in the intestinal tract and is the dominant flora in the intestinal tract. The number of bifidobacteria is closely related to human health. Sexual beneficial bacteria. The main physiological functions of bifidobacteria are: 1. Biological barrier and biological antagonism; 2. Enhance immunity and anti-tumor effect; 3. Nutritional effect; 4. Inhibit the production of endotoxin; 5. Slow down the aging of the body. With the increase of age, environmental pollution and the use of antibiotics, the number of bifidobacteria in the human intestinal tract gradually decreases, resulting in the destruction of the micro-ecological balance in the intestinal tract, leading to the occurrence of various diseases and aging. [0003] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123
Inventor 邵建宁张文齐刘彩云麻和平赵昊星魏亚琴彭章普
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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