Efficient compound biofertilizer
A bio-fertilizer and high-efficiency technology, applied in the field of fertilizers, can solve the problems of waste of fertilizer application, large dosage, high cost, etc., and achieve the effect of increasing vegetable production
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preparation example Construction
[0021] (1) Preparation of bacterial suspension
[0022] The single Li-2013 colony grown on the plate streaked and separated into the seed culture medium, 100r / min, 40 ℃ after 12 hours of culture, 1mL culture broth was centrifuged, washed twice with saline, and resuspended with 9mL physiological In salt water.
[0023] (2) Combined mutagenesis of ultraviolet light-lithium chloride-diethyl sulfate
[0024] Place the bacterial suspension in a sterile plate, stir and irradiate it under a UV lamp with a distance of 30cm and a power of 15w for 100s. The irradiated bacterial solution was applied to a lithium chloride plate after gradient dilution, and the plate was diluted with the bacterial solution without ultraviolet irradiation as a control. Wrap the above-mentioned uniformly coated plate with black cloth or newspaper, and incubate at 40°C for 48 hours. On the plate with colony growth, the one with the largest ratio of hydrolysis circle to colony diameter is selected and stored on the...
Embodiment 1
[0059] Example 1 A high-efficiency composite biological fertilizer, which is composed of Aspergillus niger culture, Bacillus megaterium phosphate, and Bacillus subtilis inoculants. The weight group of the high-efficiency composite biological fertilizer becomes: 40 parts of Aspergillus niger culture, Bacillus megaspore phosphate 8 parts of Bacillus and 20 parts of Bacillus subtilis;
[0060] The high-efficiency compound biological fertilizer is prepared by the following method:
[0061] Bacteria culture preparation:
[0062] Preparation of Bacillus subtilis agent culture: transfer and cultivate Bacillus subtilis from the inclined plane, and transfer the seed liquid after stepwise expansion into the fermentation tank. After the fermentation is completed, the fermentation liquid is vacuum-concentrated to 50% of the original volume under low temperature and negative pressure to obtain the bacteria Concentrate. Add carrier: add the mixed carrier to the concentrated solution and mix even...
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