Method of establishing efficient regeneration system of shoot tips of dendrocalamus hamiltonii

A kind of Banna sweet dragon bamboo, high-efficiency regeneration technology, applied in the field of plant tissue culture, to achieve the effects of loose implementation conditions, simple implementation steps, and significant effects

Inactive Publication Date: 2014-05-14
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

This patent application uses buds as explants to explore a high embryogenic callus induction rate, establishes an efficient an

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  • Method of establishing efficient regeneration system of shoot tips of dendrocalamus hamiltonii
  • Method of establishing efficient regeneration system of shoot tips of dendrocalamus hamiltonii
  • Method of establishing efficient regeneration system of shoot tips of dendrocalamus hamiltonii

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Embodiment Construction

[0023] The present invention is described in further detail below in conjunction with accompanying drawing and embodiment:

[0024] The steps of the method for setting up the efficient regeneration system of Banna sweet dragon bamboo bud tips are as follows:

[0025] (1) Induction culture of lateral bud callus: get lateral buds as explants from the vigorous Banna sweet dragon bamboo plant without disease spots and worm scars, rinse under tap water for 1h, and vacuum with 1% sodium hypochlorite with mass ratio concentration Suction filtration for 15 minutes, rinse with sterile water 5-6 times, cut shoot tips with a length of 1±0.2cm on the ultra-clean workbench, inoculate them in induction medium, basic medium MS (Murashige and Skoog), add extra The source hormone 2,4-D is 2,4-dichlorophenoxyacetic acid and 6-BA is 6-benzyladenine, the concentration of 2,4-D is 3-5mg·L -1 , 6-BA concentration is 0.5, 1mg L -1 , then add 500mg·L -1 CH is hydrolyzed casein, 500mg·L -1 Pro-Pro...

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Abstract

The invention relates to a method of establishing an efficient regeneration system of shoot tips of dendrocalamus hamiltonii. The method comprises the following five steps: S1, induced culture of a lateral bud callus; S2, subculture of the callus; S3, differentiation culture of a seedling; S4, rooting culture of the seedling; and S5, hardening-seedling and transplanting of a regenerated plant. The invention relates to a plant tissue culture method which takes the shoot tip of the lateral bud of the dendrocalamus hamiltonii as an explant and is a beneficial attempt of obtaining the inductivity of an efficient embryonic callus obtained through tissue culture by taking the lateral bud of the bamboo as the explant. By adopting the method, the callus can be efficiently induced from the explant to establish an efficient somatic cell regeneration system, thereby creating beneficial conditions for genetic transformation and cell engineering research of the dendrocalamus hamiltonii and laying a good foundation for breeding by mean of the transgenic technology. The method is simple and feasible, beneficial to popularize, loose in implementing condition and remarkable in effect.

Description

technical field [0001] The invention belongs to the technique of plant tissue culture, and specifically uses the bud tips of Banna sweet dragon bamboo as explants to achieve high-efficiency regeneration through callus induction, differentiation and rooting, so as to further lay a solid foundation for the genetic transformation of Agrobacterium. Background technique [0002] Banna sweet dragon bamboo (Dendrocalamus hamiltonii) belongs to the Gramineae Bamboo subfamily and is one of the three largest sweet dragon bamboos in the world. It is an excellent dual-purpose bamboo species. Due to the shortcomings of traditional breeding methods such as consumption of many bamboo species and low reproduction coefficient, the use of genetic engineering to improve bamboo varieties has become the focus and difficulty of current biotechnology research. Banna sweet dragon bamboo gene transformation is mainly Agrobacterium infection method, as the receptor system of gene transformation requi...

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Application Information

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IPC IPC(8): A01H4/00A01H5/00
Inventor 林新春周玲王晓芹
Owner ZHEJIANG FORESTRY UNIVERSITY
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