Polymer chemiluminescent labeling reagent as well as preparation method and application of reagent

A technique for polymerizing chemiluminescence and labeling reagents, which can be used in chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, scientific instruments, etc., and can solve problems such as insufficient sensitivity

Inactive Publication Date: 2014-05-14
HELIOSENSE BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although acridinium esters have better chemiluminescent properties than isoluminol, they also encounter the problem of insufficient sensitivity when detecting ultra-low concentrations

Method used

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  • Polymer chemiluminescent labeling reagent as well as preparation method and application of reagent
  • Polymer chemiluminescent labeling reagent as well as preparation method and application of reagent
  • Polymer chemiluminescent labeling reagent as well as preparation method and application of reagent

Examples

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Embodiment Construction

[0064] The following examples will further illustrate the present invention.

[0065] The three components of the present invention include a chemiluminescent label, a polymeric backbone, and a ligand or a functional group. The following example uses acridinium ester as a chemiluminescent label, and the polymer is selected from bovine serum albumin (BSA), dendrimer, polyethyleneimine (PEI), dextran (chitosan), ligand Selected from biotin (biotin, Bt), streptavidin (streptavidin, SAv), digoxin (digoxin) and the like.

[0066] ⑴ Required instruments:

[0067] Orion II Microplate Luminometer (Berthold, Germany), Berthold's Sirius L TubeLuminometer, automatic microplate washer (PW-812, Huisong), UV-Vis spectrophotometer (TU-1810, Beijing Puyi General Instrument Co., Ltd. ), a new fully automatic electric incubator (ZDP-A2080A, Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.).

[0068] (2) Required medicines:

[0069] Unless otherwise specified, the following dr...

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Abstract

The invention relates to a polymer chemiluminescent marker, and in particular relates to a polymer chemiluminescent labeling reagent as well as a preparation method and the application of the reagent. The polymer chemiluminescent labeling reagent comprises the following raw materials: a chemiluminescent marker, a polymer framework, and a ligand, a receptor or a functional group. The preparation method of the polymer chemiluminescent labeling reagent comprises the steps of (1) modifying the polymer framework; (2) modifying the ligand or the receptor; (3) synthesizing a ligand-polymer conjugate body or a receptor-polymer conjugate body; and (4) synthesizing the polymer chemiluminescent labeling reagent. The polymer chemiluminescent labeling reagent can be widely applied to the fields of medical diagnosis, biotechnology, biological medicine, life science, food safety, environmental detection, etc. Specifically, the polymer chemiluminescent labeling reagent is most commonly applied to immune analysis diagnosis, secondly applied to the analysis of the interaction between the ligand and the receptor in new drug research and development, and thirdly applied to nucleic acid or gene detection.

Description

technical field [0001] The present invention relates to a chemiluminescent labeling substance, in particular to a polymerized chemiluminescent labeling reagent and a preparation method thereof. Background technique [0002] Chemiluminescence refers to a phenomenon in which visible light is generated during a chemical reaction. It usually refers to the fact that some compounds absorb chemical energy and are excited from the ground state to the excited state by absorbing chemical energy, and then release energy when de-exciting. Photons are generated to realize the transformation from chemical energy to light energy, resulting in the phenomenon of luminescence. By measuring the light intensity of the chemiluminescence reaction, the content of trace compounds can be accurately quantified. The chemiluminescent immunoassay (CLIA) developed in recent years is the product of the combination of chemiluminescent technology and immunological methods. It not only has the high sensitiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N21/76
CPCG01N21/76G01N33/533
Inventor 刘涧泉黄炳河
Owner HELIOSENSE BIOTECH INC
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