Production method of gastrodia elata seed germinating strains

A technology of germination bacteria and Gastrodia elata, applied in the field of microorganisms, can solve problems such as low yield, poor quality, and slow growth of germinating bacteria cultivars

Active Publication Date: 2014-05-21
云南一品福生物科技有限责任公司
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Problems solved by technology

[0003] The purpose of the present invention is to shorten the production cycle of germinating bacterium cultivars by changing the production process of gastrodia elata seed germination bacteria, realize the effective dock...

Method used

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Embodiment 1

[0014] 1. Isolation of strains: In the main production area of ​​Gastrodia elata in Xiaocaoba, Yiliang, Yunnan, the source of germination bacteria was collected, isolated, purified and screened to obtain strains of germination bacteria, strain number HL-001, strain preservation number: CGMCC No.6759, classification name: Small mushroom Mycena sp., preservation time: November 05, 2012, preservation unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation unit address: Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing.

[0015] 2. Preparation of parent species: Prepare test tube slant medium, inoculate germination bacteria strains on the test tube slant medium, put them in an incubator, and cultivate at 25°C until the mycelium is covered with the medium. Each liter of test tube slant medium contains the following ingredients: 200g of potatoes, 15g of glucose, 2g of corn flour, 1...

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Abstract

The invention discloses a production method of gastrodia elata seed germinating strains. The production method is characterized in that gastrodia elata seed germinating strain cultivars are produced through a submerged fermentation technology. The production technology comprises six steps as follows: (1) separation of the germinating strains; (2) preparation of mother strains; (3) preparation of liquid seed strains; (4) submerged fermentation production; (5) preparation of cultivar culture media; and (6) inoculated culture. According to the production method, the production cycle of the cultivars is shortened, the yield of the cultivars is increased, meanwhile, various culture media for the gastrodia elata seed germinating strains are provided, the problems of inconsistent quality of the germinating strains and slowness in growth are solved, and the large-scale industrialized production of the gastrodia elata seed germinating strains is realized.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a production method of Gastrodia elata seed germination bacteria. Background technique [0002] Tianma ( Gastrodia elata Blume) is a precious traditional Chinese medicine, which belongs to Orchidaceae ( Orchidaceae ) Perennial symbiotic herbaceous plants, no roots and no green leaves, unable to carry out photosynthesis and self-supporting life. Gastrodia elata seeds are as small as dust, have no endosperm and other nutrient reserves, and no external nutrient supply. The seeds cannot germinate and must rely on germination bacteria to germinate normally. Subsequent growth requires the invasion of Armillaria fungi to provide nutrients. Continue to grow to complete its life cycle. The nutrients provided by different germination bacteria have a great influence on the germination of Gastrodia elata seeds and the growth of protocorms formed after germination. Therefore, the ...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 不公告发明人
Owner 云南一品福生物科技有限责任公司
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