Method for immobilizing cellulose crosslinking crystal on silica gel plate

A technology of cellulase and immobilization method, applied in the field of enzyme engineering, can solve problems such as complicated steps, loss of microcrystals, etc., and achieve the effects of improving utilization efficiency, reducing losses, and avoiding difficult recovery.
CN103805589AInactive Publication Date: 2014-05-21HUBEI UNIV OF TECH
2 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUBEI UNIV OF TECH
Publication Date
2014-05-21
Estimated Expiration
Not applicable · inactive patent
Patent Text Reader

Abstract

The invention discloses a method for immobilizing a cellulose crosslinking crystal on a silica gel plate, and belongs to the field of enzyme engineering. The method for immobilizing the cellulose crosslinking crystal on the silica gel plate comprises the following steps: soaking the silicon gel plate with water, cleaning and drying; uniformly mixing a cellulose solution with a precipitating agent, and placing the pretreated silicon gel plate in a mixture solution to ensure that cellulose is absorbed on the silicon gel plate; adding a crosslinking agent to crosslink to form the cellulose crosslinking crystal and immobilizing the cellulose crosslinking crystal on the silicon gel plate. According to the method, the cellulose crosslinking crystal is immobilized on the silicon gel plate, so that the activity and stability of the cellulose are improved, the cellulose can be recycled without being recovered, and the utilization efficiency of the cellulose is greatly increased.
Need to check novelty before this filing date? Find Prior Art

Description

[0001] technical field

[0002] The invention belongs to the field of enzyme engineering, and in particular relates to a method for fixing cellulase cross-linked crystals on a silica gel plate. Background technique

[0003] Cellulose is the most abundant renewable resource on the earth. The cellulose produced by photosynthesis on the earth reaches about 10 billion tons every year. The use of cellulose to produce bioenergy is the key to alleviating the energy crisis and realizing the sustainable development of human beings.

[0004] The key to cellulose utilization is to degrade cellulose into fermentable sugar-glucose. The degradation of cellulose requires the joint action of exonuclease, endonuclease and glucosidase. Degrading cellulose with cellulase has the characteristics of green, mild conditions and high conversion rate, but the high cost of enzymatic hydrolysis of cellulose has become the bottleneck of industrial cellulose degradation by cellulase.

[0005] An effe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More