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Method for immobilizing cellulose crosslinking crystal on silica gel plate

A technology of cellulase and immobilization method, applied in the field of enzyme engineering, can solve problems such as complicated steps, loss of microcrystals, etc., and achieve the effects of improving utilization efficiency, reducing losses, and avoiding difficult recovery.

Inactive Publication Date: 2014-05-21
HUBEI UNIV OF TECH
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Problems solved by technology

Similarly, the recovery of microcrystals also requires processes such as centrifugation, and the steps are cumbersome, and the microcrystals are lost during the recovery process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A Silica gel plate pretreatment

[0027] TLC silica gel plate (TLC silica gel plate G, Shanghai Yantuo Biotechnology Co., Ltd.) was soaked in deionized water for 4 h, then rinsed with deionized water three times, and dried at 25 °C for 24 h. TLC silica gel plate G was cut into 10 cm × 100 cm plates. Place the thin-layer chromatography silica gel plate G in a 10 cm×40 cm×10 cm glass box. Put the silica gel plate into the glass box, and the silica gel plate is close to the glass plate at the bottom.

[0028] B Cellulose Adsorption

[0029] Dissolve 1 g of cellulase (purchased by Sigma) in 50 mL of citric acid buffer (pH 5.0, 0.1 M), then add 100 mL of acetonitrile cooled to 4 °C and mix well, and pour the mixed liquid into a glass box. Pick up the bottom of the glass box, shake gently for 5 min, and then let stand for 20 min.

[0030] C. Immobilization of Cellulase Crosslinked Crystals

[0031] 9.2 mL of glutaraldehyde solution with a mass fraction of 50% was added t...

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Abstract

The invention discloses a method for immobilizing a cellulose crosslinking crystal on a silica gel plate, and belongs to the field of enzyme engineering. The method for immobilizing the cellulose crosslinking crystal on the silica gel plate comprises the following steps: soaking the silicon gel plate with water, cleaning and drying; uniformly mixing a cellulose solution with a precipitating agent, and placing the pretreated silicon gel plate in a mixture solution to ensure that cellulose is absorbed on the silicon gel plate; adding a crosslinking agent to crosslink to form the cellulose crosslinking crystal and immobilizing the cellulose crosslinking crystal on the silicon gel plate. According to the method, the cellulose crosslinking crystal is immobilized on the silicon gel plate, so that the activity and stability of the cellulose are improved, the cellulose can be recycled without being recovered, and the utilization efficiency of the cellulose is greatly increased.

Description

[0001] technical field [0002] The invention belongs to the field of enzyme engineering, and in particular relates to a method for fixing cellulase cross-linked crystals on a silica gel plate. Background technique [0003] Cellulose is the most abundant renewable resource on the earth. The cellulose produced by photosynthesis on the earth reaches about 10 billion tons every year. The use of cellulose to produce bioenergy is the key to alleviating the energy crisis and realizing the sustainable development of human beings. [0004] The key to cellulose utilization is to degrade cellulose into fermentable sugar-glucose. The degradation of cellulose requires the joint action of exonuclease, endonuclease and glucosidase. Degrading cellulose with cellulase has the characteristics of green, mild conditions and high conversion rate, but the high cost of enzymatic hydrolysis of cellulose has become the bottleneck of industrial cellulose degradation by cellulase. [0005] An effe...

Claims

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Application Information

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IPC IPC(8): C12N11/04
Inventor 薛栋升汪江波彭春龙周敏
Owner HUBEI UNIV OF TECH
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