Compound albumen powder for lincomycin fermentation

A technology of compound protein powder and lincomycin, which is applied in the directions of fermentation, microorganisms, biochemical equipment and methods, etc., to achieve good application value and improve the effect of fermentation titer

Inactive Publication Date: 2014-05-21
SHANGHAI INST OF PHARMA IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lincomycin and its derivatives are still clinically used antibiotics in large quantities. Therefore, it is urgent to solve the problems existing in the fermentation production of lincomycin and improve the fermentation unit.

Method used

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  • Compound albumen powder for lincomycin fermentation
  • Compound albumen powder for lincomycin fermentation
  • Compound albumen powder for lincomycin fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0024] Example 1-3: Composite protein powder is used for lincomycin fermentation and compared with the original formula

Embodiment 1

[0026] The mass percentage of the composite protein powder is composed of 25% of cold-pressed soybean powder, 30% of peanut cake powder, 15% of cottonseed protein powder and 30% of fish meal. The composite protein powder has a total nitrogen content of 52.5%, an amino nitrogen content of 14.5%, and a total phosphorus content of 1.9%.

[0027] Using the original shake flask fermentation medium (shake flask fermentation unit about 3500 μg / ml) as a control, different nitrogen sources (cottonseed protein powder, peanut cake powder, yeast powder, peptone, fish meal, corn gluten meal, yeast extract, beef extract and The composite protein powder described in Example 1) was substituted for 10 g / L cold-pressed soybean powder in the control group to investigate the effect of nitrogen source on lincomycin biosynthesis. see results figure 1 , wherein cottonseed protein powder, peptone and the composite protein powder of Example 1 of the present invention can all improve the biosynthesis ...

Embodiment 2

[0029] The mass percentage of the composite protein powder is composed of 20% of cold-pressed soybean powder, 25% of peanut cake powder, 10% of cottonseed protein powder and 45% of fish meal. The composite protein powder has a total nitrogen content of 55.5%, an amino nitrogen content of 12.5%, and a total phosphorus content of 1.6%.

[0030] Taking the original shake flask fermentation medium as a control, the composite protein powder of Example 2 was used to replace 20 g / L cold-pressed soybean powder in the original shake flask fermentation medium to investigate the effect on lincomycin biosynthesis. Finally, the fermentation titer of lincomycin can be increased to 4650μg / ml.

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Abstract

The invention discloses compound albumen powder for lincomycin fermentation. The compound albumen powder is prepared from the following components in percentage by mass: 20-25% of cold pressed soybean meal, 15-30% of peanut meal, 10-25% of cottonseed protein meal and 20-25% of fish meal. Verified by a 200L fermentation tank, the fermentation unit of the compound albumen powder disclosed by the invention can reach up to 9561 microgram/ml which is superior to the average level in China at present in a great extent. The compound albumen powder has a good application value.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular, the invention relates to a composite protein powder for lincomycin fermentation. Background technique [0002] Since the discovery of lincomycin (lincomycin) in the 1960s, the fermentation yield of lincomycin has been greatly improved. However, in the production process of general enterprises, there are common problems in the middle and late stages of fermentation that mycelium breaks severely and autolysis occurs in advance, which greatly affects the biosynthesis rate and effective biosynthesis time of lincomycin. Studies have shown that high concentrations of free amino nitrogen and dissolved phosphorus can promote cell growth and reproduction, can shorten the time required for bacterial cell reproduction, and at the same time inhibit the biosynthesis of many antibiotics, which has important guiding significance for industrial production. Chinese patent applicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/64C12R1/565
Inventor 李继安陈代杰林惠敏蓝鸿毛全贵张宏周
Owner SHANGHAI INST OF PHARMA IND
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