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Rapid real-time DNA amplifying equipment and gene mutation detection method

An amplification instrument, fast technology, applied in the direction of chemical instruments and methods, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of inability to quickly realize thermal cycle, complex structure, etc., to improve mobile flexibility and reduce vertical volume and weight, to achieve the effect of thermal cycle

Active Publication Date: 2014-05-28
重庆京因生物科技有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] One of the purposes of the present invention is to make up for the shortcomings of the existing technology that the thermal cycle cannot be quickly realized and the structure is complex, and to provide a fast real-time DNA amplification instrument that can quickly realize the thermal cycle and has a simple structure

Method used

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  • Rapid real-time DNA amplifying equipment and gene mutation detection method
  • Rapid real-time DNA amplifying equipment and gene mutation detection method
  • Rapid real-time DNA amplifying equipment and gene mutation detection method

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Embodiment Construction

[0042] The present invention will be further described in detail below in conjunction with the accompanying drawings.

[0043] see Figure 1 to Figure 9 , a fast real-time DNA amplification instrument, including a base plate 1, a reagent tank 2 and a thermal cycle system, wherein, the two ends of the reagent tank 2 are fixed on the base plate 1 by screws, and the reagent tank 2 has an insert for placing a reaction tube Hole 2a, the substrate 1 has a gap corresponding to the position of the socket 2a, and the reaction tube can be installed into the socket 2a of the reagent tank 2 through the gap; the thermal cycle system includes a heating element 3 and a radiator, and the heating element 3 and a radiator Distributed on both sides of the reagent tank 2, and the heating element 3 and the heat sink can alternately approach or move away from the reagent tank 2 driven by the driving mechanism. In this embodiment, the reagent tank 2 has twelve jacks, and the substrate 1 has square ...

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Abstract

The invention discloses rapid real-time DNA amplifying equipment and a gene mutation detection method. The rapid real-time DNA amplifying equipment comprises a reagent tank and a thermal cycling system, wherein an inserting hole for placing a reaction tube is formed on the reagent tank; the thermal cycling system comprises a heating element and a radiator; the heating element and the radiator are distributed at two sides of the reagent tank; the heating element and the radiator can alternatively be close to or depart from the reagent tank under drive of a drive mechanism. The rapid real-time DNA amplifying equipment disclosed by the invention achieves rapid heating and cooling by controlling the distances from the heating element and the radiator to the reagent tank. The design not only is simple in structure, but also can help a reagent more rapidly achieve the set temperature point, and achieves rapid thermal cycle. Meanwhile, just 50 minutes are required from sampling to successful identification of mononucleotide mutation by adopting the gene mutation method disclosed by the invention while the similar instrument on the market generally needs 2-3 hours. Thus, the detection efficiency of the gene mutation is greatly improved.

Description

technical field [0001] The invention relates to a fast real-time DNA amplification instrument and a method for detecting gene mutation through the amplification instrument. [0002] Background technique [0003] In the field of molecular biology, PCR reaction is a commonly used experimental technique. PCR reaction is the polymerase chain reaction (Polymerase Chain Reaction, PCR), which is a method for enzymatically synthesizing specific DNA fragments in vitro. It consists of high temperature denaturation, low temperature recombination and Optimal temperature extension constitutes a cycle, and the cycle is repeated, so that the target DNA can be rapidly amplified. It has the characteristics of strong specificity, high sensitivity, easy operation, and time saving. It can not only be used for gene isolation, cloning, and nucleic acid sequence analysis. research, and can also be used for disease diagnosis. [0004] The DNA amplification instrument is an instrument used for PCR...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12Q1/68
CPCB01L7/52C12Q1/6858C12Q2563/107C12Q2531/113
Inventor 杨勇黄东旗程洪斌汪钲栖
Owner 重庆京因生物科技有限责任公司
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