Bacillus licheniformis and preparation method and application of fungicide

A technology of Bacillus licheniformis and microbial inoculum, which is applied in the field of microbial pesticides and microbial fertilizers, and can solve the problems of unseen tea strains, unseen Bacillus licheniformis and the like.

Active Publication Date: 2014-05-28
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In summary, there are many strains of Bacillus licheniformis that can antagonize plant diseases, and their culture methods are also different. However, there are no strains used for tea production, and there are no reports of using sweet potato starch wastewater to cultivate Bacillus licheniformis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Production of Bacillus licheniformis microbial inoculum from sweet potato starch wastewater

[0017] Sweet potato starch wastewater pretreatment: adjust the pH of wastewater to 7.5 with lime water, and sterilize at 110°C for 15 minutes.

[0018] Preparation of liquid seeds: Inoculate the slant-preserved Bacillus licheniformis into the triangular flask containing the pretreated sweet potato starch wastewater, and shake and culture at 32° C. at 150 rpm for 26 hours to obtain liquid seeds.

[0019] Liquid fermentation: Inoculate 5% (volume ratio) of liquid seeds into the fermenter containing the pretreated sweet potato starch wastewater, and cultivate at 32 °C for 20 hours in sterile air; The number of bacteria reaches 3.5×10 9 CFU / ml.

[0020] Preparation of bacterial agent: take the biological carbon that has passed a 30-mesh sieve as a carrier, add the fermentation broth to the biological carbon according to the weight ratio of fermentation broth: biologic...

Embodiment 2

[0021] Embodiment 2: Bacillus licheniformis microbial inoculum is applied to organic tea production

[0022] In the organic tea garden, a test plot of about 2 mu where the tea trees grow evenly is selected. The management of the experimental tea trees is the same as that of other tea trees in the base, but the fertilization is different. The experimental field is divided into 5 blocks, corresponding to 5 treatments respectively, and each treatment is provided with an isolation row of about 2 meters. The inoculum is diluted with tap water or the fermentation broth is directly diluted for use. The 5 treatments are respectively the clear water control ( CK), starch wastewater control (FCK), high concentration (2.0×10 7 CFU / ml) furrow application (H), medium concentration (1.5×10 7 CFU / ml) furrow application (M), low concentration (1.0×10 7 CFU / ml) furrow application (L), according to the amount of 3 liters of inoculum dilution per square meter. In each treatment, the inocul...

Embodiment 3

[0023] Example 3: Bacillus licheniformis microbial inoculants are used to prevent damping-off of cucumbers

[0024] Cucumber damping off is a disease caused by a fungal infection. Collect the soil in the greenhouse where cucumbers have been grown for many years, mix it evenly, put it into flower pots, set up 2 groups of flower pots, each group of 10 pots, as for the greenhouse at 15 ℃ ~ 20 ℃, keep high humidity and low light. strength. The first group of pots were directly sown with cucumber seeds, 15 seeds per pot, as a control. In the second group, 0.3% by weight of Bacillus licheniformis microbial inoculum was mixed into the soil before sowing cucumber seeds, and then 15 seeds were sown in each pot. The total number of seedlings was counted after they were unearthed. After the seedlings grew one true leaf, the total number of lodging seedlings was counted before two true leaves. The results showed that the lodging rate of the control seedlings was more than 50%, while th...

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Abstract

The invention relates to a bacillus licheniformis which can prevent and treat plant disease and promote plant growth, and a low-cost preparation method of fungicide, belonging to the technical field of microbial pesticides and microbial fertilizers. The fungicide is an efficient plant growth-promoting bacteria-bacillus licheniformis B4 bacterial strain separated and screened from aquatic plant rhizosphere soil, and is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 6677. In the invention, bacillus licheniformis is cultured in large scale by using sweet potato starch wastewater as a fermentation culture medium to prepare the fungicide which can prevent and treat plant disease and promote plant growth. The process is low in preparation cost and simple to operate, and also can realize resource utilization of the sweet potato starch wastewater and reduce pollutant discharge. The prepared fungicide, used for preparation of tea leaves and vegetables, can not only improve the yield, but also improve the quality of tea gardens and vegetables, so that the bacillus licheniformis has wide application prospect.

Description

technical field [0001] The invention relates to a Bacillus licheniformis that antagonizes plant pathogenic bacteria and promotes plant growth, and a low-cost production method thereof, belonging to the technical field of microbial pesticides and microbial fertilizers. Background technique [0002] Bacillus licheniformis ( Bacillus licheniformis ) is widely distributed in nature and is an important biocontrol bacteria. It inhibits plant pathogenic bacteria, fungi, viruses and nematodes by producing active substances such as antibiotics, antibacterial proteins or polypeptides, thereby playing a role in biological control; It is also an important type of plant rhizosphere growth-promoting bacteria, showing the effect of promoting the growth of a variety of plants. For example, a Chinese patent for invention (application number: 200310115535.5) discloses a strain of Bacillus licheniformis B-0A12 with a deposit number of CGMCC No. 1035 for preventing and treating soybean cyst ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14A01N63/00A01P3/00A01P21/00C05F11/08A01G13/00A01G7/06C12R1/10
CPCY02A40/10
Inventor 白志辉张茉莉陈月月赵志瑞庄绪亮杨建州
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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