Multiplex amplification kit for 25 STRs (short tandem repeats)

A technology of compound amplification and compound amplification system, which is applied in the field of simultaneously amplifying multiple short tandem repeat loci and detecting polymorphic genetic marker genes in the human genome, which can solve the waste of DNA database data resources and the effectiveness Insufficient problems, etc., to achieve the effects of reduced cost of detection reagents, reduced detection time, and high compatibility

Active Publication Date: 2014-05-28
BEIJING PEOPLESPOT TECH
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Therefore, due to the different loci of each STR kit, some data resour

Method used

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  • Multiplex amplification kit for 25 STRs (short tandem repeats)
  • Multiplex amplification kit for 25 STRs (short tandem repeats)
  • Multiplex amplification kit for 25 STRs (short tandem repeats)

Examples

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Embodiment 1

[0046] Example 1 Compound amplification of 25 gene loci and analysis of their genotypes

[0047] Blood is donated by volunteers. The template DNA was extracted from the blood using the chelex-100 method, and the forensic typing standard 9948 was added. The amplification reaction was carried out on the ABI9700 thermal cycler, the electrophoresis and detection were carried out on the ABI3100 genetic analyzer, and the data analysis was performed using GeneMapper ID v3.2 software. The samples were detected by the SinoFiler kit from ABI company and the Goldeneye20A kit from Genepoint Cognitive Co., Ltd., and the operation method was carried out according to the instructions of the kits, and the results were used as a control. The reagents and materials used in the present invention, such as allelic ladder (ladder), are conventional materials commonly used by those skilled in the art.

[0048] 1.1. chelex-100 method to extract DNA (specific method refer to "Forensic DNA Protocol",...

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Abstract

The invention relates to a multiplex amplification system for analyzing a plurality of STR (short tandem repeat) gene loci simultaneously. The system is characterized in that 25 gene loci are subjected to multiplex amplification and include: Amelogenin, vWA, D10S1248, D2S441, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D22S1045, D1S1656, D19S433, D6S1043, D12S391, D8S1179, D3S1358, CSF1PO, Penta D, Penta E, TH01, TPOX and DYS391. The invention further relates to a method and a kit for analyzing DNA (deoxyribonucleic acid) samples and applications of the method and the kit.

Description

Technical field: [0001] The present invention relates to the field of biotechnology. Further, the present invention relates to the detection of polymorphic genetic marker genes in the human genome. In particular, the present invention relates to the simultaneous amplification of multiple short tandem repeat loci in one amplification system using polymerase chain reaction. Background technique: [0002] Short tandem repeats (STR for short), also known as microsatellites or simple sequence repeats (SSR), are a type of DNA tandem repeats widely present in eukaryotic genomes, with a core sequence of 2-6 base repeat units. The number of STR loci is large and the division is extensive, accounting for about 3% of the entire genome (International Human Genome Sequencing Consortium, 2001), and the polymorphism is high, and its polymorphism is mainly derived from the difference in the number of core sequence repeats between individuals. And this difference follows the Mendelian law ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 张威张琰
Owner BEIJING PEOPLESPOT TECH
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