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β-glucosidase high-producing bacteria and its application in the transformation and preparation of resveratrol

A technology of glucosidase and resveratrol, which is applied to β-glucosidase high-producing bacteria and its application field in the transformation and preparation of resveratrol, can solve the problems of high cost, complicated process, restricted development and the like

Inactive Publication Date: 2016-12-07
YULIN NORMAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

Although the β-glucosidase gene has been successfully cloned and expressed, and the newly constructed engineering bacteria have also been applied to production practice, there are still many deficiencies, such as: complex process and high cost, which limit its further development

Method used

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  • β-glucosidase high-producing bacteria and its application in the transformation and preparation of resveratrol
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  • β-glucosidase high-producing bacteria and its application in the transformation and preparation of resveratrol

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Embodiment Construction

[0022] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.

[0023] 1. Screening of strains

[0024] Soil samples were taken from the landfill in Yulin Traditional Chinese Medicine Port, Guangxi. 10% Polygonum cuspidatum solution was used as the enzyme reaction substrate, and the improved Cha's medium (sucrose was removed in Cha's medium) was used to form a primary screening medium. Congo Red staining method stains the transparent circle that appears, and judges whether there is the fungal strain that produces β-glucosidase, and the process is:

[0025] (1) Preliminary screening of strains: Take 5g of the collected soil sample, add it to a triangular flask filled with 45mL sterile water, shake for 5min, make a soil suspension, and dilute it in gradient for 10 -1 ~10 -7 times. Select the appropriate dilution factor of the bacterial suspension and spread it on the primary screening plate medium, place ...

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Abstract

The invention discloses a beta-glucosidase high-yielding strain which is filtered from the nature by taking polygonum cuspidatum as a carbon source, wherein the collection number of the beta-glucosidase high-yielding strain is CCTCC (China Center for Culture Collection) NO.M2013633; the beta-glucosidase high-yielding strain is classified and named as Aspergillus oryzae HHL; the strain is capable of efficiently converting polydatin into resveratrol. Accordingly, the invention discloses a method for converting and preparing resveratrol by using the beta-glucosidase high-yielding strain, on one hand, the polydatin can be hydrolyzed in a mild condition so that the defect of loss of effective constituents caused by the traditional method is avoided and then the yield is effectively improved, and on the other hand, by utilizing the modern microbial fermentation engineering technology and enzyme engineering technology, the effective constituent polydatin of the Aspergillus oryzae is converted in vitro in scale under controllable conditions so that the production cost is reduced and the production efficiency is improved. According to the beta-glucosidase high-yielding strain, a feasible way is provided for the massive industrial production of beta-glucosidase and resveratrol, higher economical benefit and social benefit are provided, and the market development prospect is broad.

Description

technical field [0001] The invention belongs to the technical field of microbial biotransformation, and in particular relates to a β-glucosidase high-yielding bacterium and its application in transforming and preparing resveratrol. Background technique [0002] Resveratrol is a natural active substance in the Chinese herbal medicine Polygonum cuspidatum. , the molecular formula is C 14 h 12 o 3 . Resveratrol in Polygonum cuspidatum often exists in the form of glucoside (ie polydatin), and polydatin can be decomposed into resveratrol under the action of glycosidase in the intestinal tract to exert pharmacological effects. Resveratrol has anti-tumor, anti-oxidation, anti-free radical, anti-thrombosis, anti-allergic, anti-atherosclerosis and prevention of coronary heart disease, ischemic heart disease, hyperlipidemia, etc. Resveratrol has been listed It is one of the most promising anti-cardiovascular and anti-cancer drugs, and it is another green anti-cancer substance aft...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/22C12R1/69
Inventor 苏龙覃荣燕区善芬许译中
Owner YULIN NORMAL UNIVERSITY
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