Colloidal gold test paper for rapidly and quantificationally detecting galectin-3

A technology of galectin and colloidal gold test paper, which is applied in the field of clinical medical diagnosis, can solve the problems of high cost, long detection time, and high requirements for detection equipment, and achieve the effect of convenient portability and convenient storage

Active Publication Date: 2014-06-18
RELIA BIOTECH SHENZHEN LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the method for detecting galectin-3 (Gal-3) is mainly enzyme-linked immunosorbent technique (ELISA). ELISA technology has the following disadvantages: high requirements for detection equipment, high cost; many interference factors, and poor repeatability ;Long detection time
In view of the fact that there is no rapid quantitative detection method at present, the purp...

Method used

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  • Colloidal gold test paper for rapidly and quantificationally detecting galectin-3
  • Colloidal gold test paper for rapidly and quantificationally detecting galectin-3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] [Example 1] Preparation of colloidal gold test paper for rapid quantitative detection of galectin-3

[0026] Such as figure 1 The colloidal gold test paper structure shown comprises a support pad 5, a nitrocellulose membrane 1 positioned on the support pad, one end of the nitrocellulose membrane is laminated and connected to the absorbent pad 4, and the other end is laminated and connected to the conjugate Gasket 2, the coupling gasket is laminated and connected to the sample gasket 3 above one end away from the nitrocellulose membrane 1, the other end of the sample gasket is glued with double-sided tape 7 below, and the top with single-sided tape 6 , it is characterized in that, the detection line 8 in the middle of the nitrocellulose membrane 1 is coated with Gal-3 antibody, the control lines 9 are respectively arranged on both sides of the detection line, and the coupling gasket 2 is coated with gold-labeled Gal-3 antibody. -3 monoclonal or polyclonal antibodies and...

Embodiment 2

[0032] [Example 2] Galectin-3 was rapidly and quantitatively detected with the colloidal gold test paper of the present invention.

[0033] Get a preferred colloidal gold test paper for rapid quantitative detection of galectin-3 of the present invention, such as figure 1 As shown, the colloidal gold test paper includes a support pad 5, and a nitrocellulose membrane 1 positioned on the support pad, one end of the nitrocellulose membrane is laminated and connected to the absorbent pad 4, and the other end is laminated and connected to the coupling compound. Gasket 2, the coupling gasket is laminated and connected to the sample gasket 3 above one end away from the nitrocellulose membrane 1, the other end of the sample gasket is glued with double-sided tape 7 below, and the top with single-sided tape 6 , it is characterized in that, the detection line 8 in the middle of the nitrocellulose membrane 1 is coated with Gal-3 antibody, the control lines 9 are respectively arranged on both...

Embodiment 3

[0034] [Example 3] Galectin-3 was rapidly and quantitatively detected using the colloidal gold test kit of the present invention.

[0035] Get a preferred colloidal gold test paper box for rapid quantitative detection of galectin-3 of the present invention, such as figure 1 with figure 2 As shown, the colloidal gold test paper includes a support pad 5, a nitrocellulose membrane 1 positioned on the support pad, one end of the nitrocellulose membrane is connected to the absorbent pad 4, and the other end is connected to the couple pad 2, The coupling gasket is connected to the sample gasket 3, the bottom of the other end of the sample gasket is bonded with double-sided adhesive tape 7, and the top is bonded with single-sided adhesive tape 6. It is characterized in that the middle part of the nitrocellulose membrane 1 The detection line 8 is coated with Gal-3 antibody, and the two sides of the detection line are respectively provided with control lines 9, and the conjugate gask...

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Abstract

The invention discloses a piece of colloidal gold test paper for rapidly and quantificationally detecting galectin-3. The colloidal gold test paper comprises a support gasket 5 and a nitrocellulose membrane 1 on the support gasket, wherein one end of the nitrocellulose membrane is connected with a water absorption pad 4, and the other end of the nitrocellulose membrane is connected with a conjugate gasket 2; the conjugate gasket is connected with a sample gasket 3; a first fixing part 7 is arranged under the other end of the sample gasket, and a second fixing part 6 is arranged above the other end of the sample gasket. The colloidal gold test paper is characterized in that a detection line 8 in the middle of the nitrocellulose membrane 1 is coated with a Gal-3 antibody; two control lines 9 are arranged at the two sides of the detection line, respectively; the conjugate gasket 2 is coated with a gold labeled Gal-3 monoclonal antibody or polyclonal antibody and a control line conjugate.

Description

technical field [0001] The invention belongs to the field of clinical medical diagnosis, and in particular relates to a colloidal gold test paper for quantitative detection of galectin-3 (Gal-3). Background technique [0002] Galectin-3 (Gal-3), also known as galactose-binding protein-3, has a molecular weight of 31kD and is one of the important members of the galectin family. Members of the Galectin family need to meet two criteria: first, affinity for galactose; second, significant sequence similarity in the carbohydrate recognition domain (CRD). Galectin-3 has two functional domains: the carboxy-terminal is the sugar recognition domain, which contains 135 amino acid residues; the amino-terminal contains 100-150 amino acid residues, which are connected in series by 9 amino acid residues rich in proline and glycine Repeat composition. Galectin-3 is mainly expressed in macrophages, eosinophils, neutrophils and mast cells. Its function depends on its localization in the ce...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/558G01N33/68G01N2333/4724G01N2800/325
Inventor 刘红剑赵俊平吴伟云夏宣喜
Owner RELIA BIOTECH SHENZHEN LTD
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