Preparation method and application of Chinese giant salamander phalanx section

A technique of phalangeal and slicing, which is applied in the field of preparation of slices of phalanges of Chinese giant salamander, and can solve problems such as differences in results, incomplete generalization of methods for amphibians without tails and amphibians with tails, gaps in age identification, etc.

Inactive Publication Date: 2014-06-25
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The age identification of the Chinese giant salamander has not been reported so far. Looking at the age identification of other amphibians, it is found that the correlation between the head and body length of each age can be used to obtain the morphological standards for age identification, but most of them are carried out using bones. The methods of amphibians and tailed amphibians are not completely universal, and the results are different
Giant salamander is an important protective economic animal, and there have been many studies on its biological characteristics, but its age identification is still blank

Method used

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  • Preparation method and application of Chinese giant salamander phalanx section

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method for Chinese giant salamander phalanx slices, the steps are as follows:

[0028] 1 Materials and methods

[0029] 1.1 Apparatus and reagents

[0030] Surgical scissors, tweezers, beaker, glass slides, cryostat (Leica, Germany), cryosection embedding agent OTC (Leica, Germany), staining cup.

[0031] 3% nitric acid solution, hematoxylin dye solution, eosin dye solution, 0.8% hydrochloric acid ethanol solution.

[0032] 1.2 Method

[0033] 1.2.1 Take the second joint of the giant salamander phalanx as the research object to make slices, and observe the age results of the giant salamander.

[0034] 1.2.2 Take giant salamander samples of known age and use this method for age identification.

[0035] Specific steps are as follows:

[0036] (1) Sampling: The toe bone samples of 4-year-old Chinese giant salamander were fixed in Bouin's solution (Bouin's) at room temperature for 24 hours. The formula of Born's solution is 75ml of picric acid, 25ml of a...

Embodiment 2

[0044] A preparation method of Chinese giant salamander phalanx slices, the steps are as follows:

[0045] 1 Materials and methods

[0046] 1.1 Apparatus and reagents

[0047] Surgical scissors, tweezers, beaker, glass slides, cryostat (Leica, Germany), cryosection embedding agent OTC (Leica, Germany), staining cup.

[0048] 3% nitric acid solution, hematoxylin dye solution, eosin dye solution, 0.8% hydrochloric acid ethanol solution.

[0049] 1.2 Method

[0050] 1.2.1 Take giant salamander phalanges as the research object to make slices and observe the age results of giant salamander.

[0051] 1.2.2 Take giant salamander samples of unknown age and use this method for age identification.

[0052] Specific steps are as follows:

[0053] (1) Sampling: The giant salamander phalanges of unknown age were fixed in Bouin's solution (Bouin's) at room temperature for 72 hours.

[0054] The formula of Born's solution is: mix 75ml of picric acid, 25ml of anhydrous formaldehyde and 3m...

Embodiment 3

[0061] A kind of application of the preparation method of Chinese giant salamander phalanx slice, its steps are as follows:

[0062] The slices prepared in Examples 1 and 2 were observed under a microscope, and the age was identified according to the number of annual ring marks on the slices.

[0063] From figure 1 It can be seen from the figure that, using the method of the present invention, it can be clearly seen that there is a group of concentric circles in the giant salamander skeleton. The concentric circles are blue-black after being stained with hematoxylin, and the outline is obvious but there will be incomplete closure. The circles The interphase is a deep blue-pink color displayed by both eosin and hematoxylin. The concentric circles are clearly identifiable, which is the so-called "bone age". The bone age of the known age identified by the method of the present invention is 4 years old; the bone age of the unknown giant salamander is 10 years old.

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Abstract

The invention discloses a preparation method and application of a Chinese giant salamander phalanx section. A second joint of a Chinese giant salamander phalanx is used as a section material and is subjected to sampling, hardening, decalcification, section cutting, dyeing and dehydration hyalinization to prepare the Chinese giant salamander phalanx section. The second joint of the Chinese giant salamander phalanx, which is used as the section material, is longer and is more uniform in thickness compared with a first joint and a third joint, and thus the profile of the section can be easily fixed and observed; in the aspect of a dyeing method, the preparation method specifically adopts a combination of different steps according to the characteristics of steps of existing various dyeing methods, and thus the color development of the texture is clear; absolute ethyl alcohol soaking and xylene are additionally arranged, and the color development result is improved.

Description

technical field [0001] The invention belongs to the field of ecological research, and in particular relates to a preparation method and application of Chinese giant salamander phalanx slices. Background technique [0002] Age identification is an important basis for studying the ecology of animal populations. Accurate age data are required for individual growth, population replenishment, and death calculations of animals. Animal age identification has a long research history. The bone age identification method is based on the age structure analysis of countable growth lines (lines of arrested growth, LAGs) on animal bone slices. As early as the 18th century, scientists began to use the bone structure of fish to determine the age of fish. It is the most traditional and commonly used method to identify the age by using the ring pattern on the hard bone tissue. The identification of annual rings on the bone structure is based on the following theory: fish in summer absorb a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
Inventor 孟彦刘小召肖汉兵田海峰胡乔木
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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