A device for creating anaerobic conditions

Abstract

The invention discloses a device for building the anaerobic condition. The device mainly comprises an oxidation rod, a copper guide tube, an alcohol blast burner, a digital display screen, an integrated circuit board module, a miniature vacuum pump, an automatic control valve, an automatic control tee joint, a condenser, an air drier, a nitrogen flowmeter, a heat exchange hole, a heat absorption chamber, an oxidation chamber, a box body, a rubber hose and the like. The device can be used for building the anaerobic condition by virtue of two modes; with the adoption of the first mode, the effect of removing oxygen in air in a limited space is reached by consuming oxygen in the air by virtue of the oxidization reaction of a copper net; with the adoption of the second mode, the air is exhausted by virtue of a mode of vacuum pump sucking and nitrogen injecting, and the anaerobic condition is built; the two modes are both realized by virtue of an automatic control mode. Compared with the traditional mode of deoxidizing and anaerobic condition acquiring, the device has the advantages of high accuracy, good effect, little time consumption, convenience in operation, high efficiency and the like and is suitable for acquiring the anaerobic environment in soils and other correlated researches.

Description

technical field [0001] This patent relates to a device for creating anaerobic conditions, specifically a device that relies on copper oxidation to remove oxygen in the air or relies on nitrogen to replace air to create an anaerobic environment. Background technique [0002] The aerobic oxidation of methane-oxidizing bacteria in soil has achieved many research results. Usually, the cultivation conditions of soil aerobic methane oxidation are easy to realize and control. However, there are few reports on the anaerobic oxidation of methanotrophs in soil, and this aspect of research has gradually become a hot spot in this field. Usually, the laboratory study of methane oxidation is carried out by using a serum bottle with a stopper for cultivation. A certain amount of soil is placed in the bottle, and a certain amount of CH is injected after the stopper is tightened. 4 , by analyzing CH 4 Concentration changes to study the oxidation of methanotrophs. [0003] In the study of ...

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Problems solved by technology

But adopting the method for exhausting air with nitrogen has many disadvantages: the first, the average molecular mass of air is 29g / mol, N 2 The average molecular mass is 28g / mol, and the average molecular mass is not much different. In this case, there is N 2 Mixing with air, the laboratory usually adopts the 5-minute nitrogen exhaust method, which does not guarantee that the air is completely excluded; second, some studies have also shown that N 2 The existence of methanotrophs may have a certain impact on the methane oxidation of methanotrophs. If N 2 Air removal method, N in culture flask 2 Will have an impact on anaerobic experimental research

[0004] At present, the creation of anaerobic culture conditions in the laboratory is basically attached to anaerobic culture tanks, anaerobic reaction bags and anaerobic incubators, but none of these equipment can meet the requirements of this research for a large number of batch cultures, that is, methane-oxidizing bacteria Anaerobic oxidation research usually involves more control conditions and must include parallel groups. In this case, the number of culture bottles can usually reach about 40-150, so culture tanks, reaction bags and incubators cannot meet the experimental requirements at all.

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