Method for preparing biological reagent with melia azedarach fruits
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A technology of biological preparations and neem fruit, applied in the field of biological preparations
Inactive Publication Date: 2014-07-16
安徽省淮北市农药厂
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[0004] Although various methods for preparing biological preparations have been disclosed in the prior art, there are no reports on how to utilize neem fruit, how to utilize the fermentation process, what kind of strains to use for fermentation, and how to prepare biological preparations after fermentation.
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Embodiment 1
[0025] The biological preparation prepared in the step 3 is water emulsion, and its preparation process: take 1-9% of fermentation broth, 3-10% of solvent, 5-15% of emulsifier, 5-10% of dispersant, 1-10% of antifreeze 5%, defoamer 0.05-0.3%, PH regulator 1-2%, deionized water 50%-85%, the fermentation broth, solvent, emulsifier are mixed and dissolved into a uniform oil phase, the dispersant, antifreeze , defoamer, and deionized water are mixed to form a uniform water phase, and the water phase is added to the oil phase under high-speed stirring, and a pH regulator is added to obtain a well-dispersed wolfba water emulsion.
Embodiment 2
[0027] The biological preparation prepared in the step 3 is a suspension agent, and its preparation process: take 1-10% of the fermentation broth, 5-10% of the dispersant, 2-5% of the suspension stabilizer, 1-5% of the antifreeze agent, defoaming Agent 0.05-0.3%, PH regulator 1-2%, deionized water 50%-85%, the above materials are put into the mixing pulper for pre-mixing, and then pumped into the first sand mill, the second sand mill, Homogenous mixer pulverizes homogeneously to obtain chamaejasma suspension concentrate.
Embodiment 3
[0029] The biological preparation prepared in the step 3 is a microemulsion, and its preparation process: take 1-9% of the fermentation broth, 3-10% of the solvent, 5-15% of the emulsifier, 3-5% of the cosolvent, and 1-5% of the antifreeze agent. 5%, stabilizer 3-5%, deionized water 50%-80%, mix the emulsifier and deionized water to form a uniform water phase, and stir the fermentation broth, solvent, antifreeze, co-solvent and stabilizer at high speed Add water phase under the state, make chamaejasma microemulsion.
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Abstract
The invention discloses a method for preparing a biological reagent with melia azedarach fruits. The method comprises the following steps: firstly, treating raw materials, namely, cleaning collected melia azedarach fruits to remove sundries on the surfaces, and airing for later use; secondly, carrying out fermentation detoxication, namely, putting the treated raw materials in the first step into a fermenting chamber, slowly adding sweet water and stirring so as to enable the ratio of materials to liquid is 1:(1.2-1.5), then adding zygosaccharomyces bailii and acetobacter xylinum with the material and liquid total quantity of 0.1-0.5% until the mixing ratio of the Bayer combined saccharomycetes to the acetobacter xylinum to be 1:(0.8-1), keeping the temperature to be 26-30 DEG C, and fermenting for 3-6 days; and thirdly, preparing the biological reagent, namely, filtering and removing particles in the fermentation liquid taken on the upper layer in the second step, and combining the fermentation liquid, auxiliaries and water in proportions to prepare the biological reagent. The zygosaccharomyces bailii and the acetobacter xylinum are used for fermenting the melia azedarach fruits, so that melia azedarach alkali is greatly analyzed; the prepared biological reagent can be taken as a broad-spectrum insecticidal bacterium reagent, and is free of residues.
Description
technical field [0001] The invention relates to a method for preparing biological preparations from neem fruit, which belongs to the technical field of biological preparations. Background technique [0002] Neem, a deciduous tree of the genus Neem. Distributed in Hebei, Shanxi, Shaanxi, Gansu, Taiwan, Sichuan, Yunnan, Hainan and other provinces. The wood is light and soft, easy to process, and is used for making furniture and farm tools; flowers, leaves, seeds and root bark can be used as medicine; it is also a street tree, ornamental tree and afforestation tree species in coastal areas. [0003] Neem tree bears a large amount of fruit, and the skin of neem tree seeds is light yellow and slightly wrinkled. Lidong matures and does not fall for a long time after ripening. Therefore, its utilization rate is very low; at present, the pesticides of pure chemical preparations are less and less accepted by people due to pesticide residues, and it has become a trend for biological...
Claims
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Application Information
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