36 results about "Zygosaccharomyces bailii" patented technology

The invention discloses aldehyde ketoreductase bacterial strain, aldehyde ketoreductase gene, a vector, engineering bacteria and an application thereof. A nucleotide sequence of aldehyde ketoreductase gene is one of a SEQ ID No. 1, a SEQ ID No. 3 and a SEQ ID No. 5, aldehyde ketoreductase gene shown by the SEQ ID No. 1 and the SEQ ID No. 3 comes from bacterial strain-kluyveromyces lactis XP1461, and aldehyde ketoreductase gene shown by the SEQ ID No. 5 comes from bacterial strain-baeyer zygosaccharomyces bailii XP1462. The disclosed aldehyde ketoreductase from K.lactis and Z. bailii can be firstly reported for biological catalyzed synthesis of 6-cyan-(3R, 5R)-dihydroxyhexanoate, optical voidness of the prepared sample 6-cyan-(3R, 5R)-dihydroxyhexanoate can be reached, existence of 6-cyan-(3R, 5R)-dihydroxyhexanoate can be hardly detected; high epimerase selectivity of aldehyde ketoreductase brings benefit, and the products extraction yield is high.

The invention provides a traditional Chinese medicine microbial ecological agent for preventing and treating infectious bursal disease of chicken and a preparation method thereof. The preparation method comprises the following steps: adding water to radix isatidis, honeysuckle, forsythia, folium isatidis, humulus scandens, radix scutellariae, dangshen, ageratum, plantain, astragalus membranaceus, angelica sinensis and liquorice, boiling by using high heat, preserving heat for 30-60 minutes by using slow fire, cooling, filtering, collecting filtrate, adding white sugar, boiling, cooling to a normal temperature, then inoculating lactobacillus, zygosaccharomyces bailii and acetobacter xylinum, and fermenting for 3-7 days so as to obtain the microbial ecological agent. After the microbial ecological agent is diluted proportionally, the diluted microbial ecological agent is directly drunk by chicken. The traditional Chinese medicine microbial ecological agent provided by the invention has the effects of resisting viruses, inducing interferon, increasing the quantity of effective microbial communities in intestinal tracts, boosting immunity and improving disease resistance of chickens without toxic or side effects and the like; the preparation method is simple, convenient to use and high in viable bacteria content and has no residues. The traditional Chinese medicine microbial ecological agent provided by the invention has a certain curative effect on infectious bronchitis and henpox and relatively good market prospect.

The invention discloses a production method and feedstuff of Chinese herbal medicinal food ducks. The feedstuff is produced according to the following method: boiling rhizoma atractylodis, witloof, pericarpium citri reticulatae, serissa serissoides, angelica sinensis, codonopsis pilosula, poria cocos, herba epimedii, and fennel in water with strong fire and then preserving heat for 1-2 hours with soft fire; cooling, filtering and collecting the filtrate; adding white sugar and zygosaccharomyces bailii to obtain fermentation broth after being fermented; and adding corn, bran and bean pulp into the fermentation broth. The feedstuff can be directly fed to ducks, and can improve the immune function of the ducks and prevent diseases. In the production method of the Chinese herbal medicinal food ducks, no antibiotic is used in the entire feeding process of the ducks, no antibiotic or hormone is remnant in the duck products, thereby being safe to the huamn body of an eater, and being beneficial to the health of the body. The Chinese herbs are fed to the ducks after fermentation, so that the content of effective constituents of the Chinese herbs is increased in fold, the sweet flavor permeates into the duck meat, and the amino acid content in the duck meat is increased. Therefore, the taste of the duck meat is changed, the duck meat is stronger in sweet flavor, and the conventional roast ducks, boiled salted ducks and aromatic ducks are changed. Meanwhile, the Chinese herbal medicinal food ducks have the functions of replenishing blood and preventing and curing diseases, and have both dietetic invigoration effect and pharmaceutical invigoration effect.

The invention relates to a quick qualitative detection technology on Zygosaccharomyces bailii in grape wine and fruit juice and in particular relates to a primer and a probe which are used for Zygosaccharomyces bailii detection and a detection method thereof. According to the invention, a 26S ribosome RNA gene sequence of Zygosaccharomyces bailii is selected, a specific primer and probe set is designed, and simple and quick detection on Zygosaccharomyces bailii in the grape wine and fruit juice can be realized by adopting the primer and the probe and a real-time fluorescent PCR technology.

The invention discloses a preparation method of antioxidant-rich Kombucha tea. The preparation method comprises the following steps of mixing and boiling water, cane sugar and black tea leaves according to the weight ratio of (125-130) to (12.5-13) to (1-1.5), wherein the obtained liquid is a base liquid; inoculating the base liquid into a seed solution prepared by mixing an acetobacter pasteurianus seed solution with the concentration of 105-107 CFU/mL, an acetobacter xylinum seed solution with the concentration of 106-108 CFU/mL and Zygosaccharomyces bailii with the concentration of 105-107CFU/mL according to the volume ratio of 1 to 1 to 1 to obtain a solution to be fermented, and the volume ratio of the mixed seed solution to the base liquid is (3-3.5) to (97-97.5); placing the solution to be fermented in a container with a gauze covered at an open end, and executing standing and fermentation under the conditions of 30%-40% humidity and 25-30 DEG C for 10 days to obtain the Kombucha tea. The fermentation period of the Kombucha tea is shortened, the content of total flavones and total phenols in the obtained Kombucha tea is high, and the oxidation resistance is strong.

The invention relates to the technical field of white spirit brewing, in particular to a quantitative analysis method of important saccharomycetes Zygosaccharomyces bailii in a white spirit solid-state fermentation process. According to the invention, a specific primer is adopted to carry out quantitative PCR analysis on 26S rRNA of Zygosaccharomyces bailii, so as to analyze the variation trend ofZygosaccharomyces bailii in Daqu and fermented grains and predict the alcohol yield. The method disclosed by the invention has the advantages of high detection speed, high sensitivity, good accuracy,good specificity and no culture.

The invention discloses a clinical mixed preparation for a cardiovascular department and a preparation method, and belongs to the field of traditional Chinese medicine. The effective component of the clinical mixed preparation is prepared through the steps that radix angelica sinensis, ligusticum wallichii, safflower carthamus, salvia miltiorrhiza, American ginseng, panax notoginseng, cortex moutan radicis, lumbricus, leeches and gadflies are decocted and then filtered, zygosaccharomyces bailii is added in obtained filtrate and medicine dregs, and the zygosaccharomyces bailii is cultured for 168-240 hours in a standing mode in a corresponding temperature and humidity environment to obtain oral liquid or orally-taken tablets or capsules. The clinical mixed preparation mainly treats heart and cerebral vusculer diseases, hypertension and blood diseases, is directly and orally taken and is capable of quickly achieving the effects, free of relapse, rapid to absorb, high in treating effect and sweet in taste, and no sequela or complication occurs after the clinical mixed preparation is taken; two special technologies of solid fermentation and liquid fermentation are achieved in the traditional Chinese medicine through microorganism application, the toxic and side effects of the raw material medicine are removed, storing and eating are convenient, and no waste medicine dreg is generated. The clinical mixed preparation is rich in raw material source, easy to prepare, low in cost, capable of achieving both treatment and health care and suitable for treating, rehabilitating and nursing of cardiovascular department diseases.

The invention discloses a method for preparing a bactericide from bran, which comprises the following steps: dissolving sugar in water to form a sugar solution; mixing Saturnospora dispora, Zygosaccharomyces bailii and Acetobacter xylinum to form a mixed bacterium solution; mixing the sugar solution and bacterium solution to form a mixed solution, and putting the bran into a fermenting tank layer by layer, wherein the mixed solution is uniformly sprayed on each layer until the layer is moistened; fermenting at constant temperature, filtering the fermented liquor to obtain fermentation liquor and solid residues, adding sodium hydroxide into the obtained fermentation liquor to regulate the pH value, and filtering to obtain a mixture containing apigenin (belonging to flavones), 5-nonadecyl resorcinol, 5-heneicosyl resorcinol and 5-tricosyl resorcinol (belonging to alkyl resorcinols), and pentosan, chitosan, oligosaccharide, amino oligosaccharide, lentinan and pujuxitang; and preparing the plant-source agricultural bactericide. The preparation technique is simple and suitable for large-scale production; and the plant-source agricultural bactericide has the advantages of high safety, environmental protection and no pesticide residue.

The invention discloses a method for preparing a biological reagent with melia azedarach fruits. The method comprises the following steps: firstly, treating raw materials, namely, cleaning collected melia azedarach fruits to remove sundries on the surfaces, and airing for later use; secondly, carrying out fermentation detoxication, namely, putting the treated raw materials in the first step into a fermenting chamber, slowly adding sweet water and stirring so as to enable the ratio of materials to liquid is 1:(1.2-1.5), then adding zygosaccharomyces bailii and acetobacter xylinum with the material and liquid total quantity of 0.1-0.5% until the mixing ratio of the Bayer combined saccharomycetes to the acetobacter xylinum to be 1:(0.8-1), keeping the temperature to be 26-30 DEG C, and fermenting for 3-6 days; and thirdly, preparing the biological reagent, namely, filtering and removing particles in the fermentation liquid taken on the upper layer in the second step, and combining the fermentation liquid, auxiliaries and water in proportions to prepare the biological reagent. The zygosaccharomyces bailii and the acetobacter xylinum are used for fermenting the melia azedarach fruits, so that melia azedarach alkali is greatly analyzed; the prepared biological reagent can be taken as a broad-spectrum insecticidal bacterium reagent, and is free of residues.

The invention discloses a method for preparing a bactericide from rice hulls, which comprises the following steps: uniformly mixing bran and rice hulls, and dissolving sugar in water to form a sugar solution; mixing Saturnospora dispora, Zygosaccharomyces bailii and Acetobacter xylinum; putting the mixture into a fermenting tank layer by layer, wherein the mixed solution of the sugar solution and mixed bacterium solution is uniformly sprayed on each layer until the layer is moistened; fermenting at constant temperature, filtering the fermented liquor to obtain fermentation liquor and solid residues, adding sodium hydroxide into the obtained fermentation liquor to regulate the pH value, and filtering to obtain a mixture containing apigenin (belonging to flavones), 5-nonadecyl resorcinol, 5-heneicosyl resorcinol and 5-tricosyl resorcinol (belonging to alkyl resorcinols), and pentosan, chitosan, oligosaccharide, amino oligosaccharide, lentinan and pujuxitang; and preparing the plant-source agricultural bactericide by using the mixture. The preparation technique is simple and suitable for large-scale production; and the plant-source agricultural bactericide has the advantages of high safety, environmental protection and no pesticide residue.

The invention discloses a probe and a method for absolute quantification of zygosaccharomyces bayer and application of the probe and the method, and belongs to the fields of biology, fermentation and detection. According to the zygosaccharomyces bayer quantitative probe and the kit, total quantity detection of zygosaccharomyces bayer can be achieved, expensive instruments are not needed when the zygosaccharomyces bayer quantitative probe and the kit are used for detection and zygosaccharomyces bayer quantification, and quantification work can be rapidly completed within 2.5 h. Meanwhile, nucleic acid extraction does not need to be carried out on a sample used in the method. Based on the probe and the detection kit provided by the invention, the detection kit is used for quantifying the zygosaccharomyces bayer and has the characteristics of rapidness, convenience, cheapness and accuracy.