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36 results about "Zygosaccharomyces bailii" patented technology
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Zygosaccharomyces bailii is a species in the genus Zygosaccharomyces. It was initially described as Saccharomyces bailii by Lindner in 1895, but in 1983 it was reclassified as Zygosaccharomyces bailii in the work by Barnett et al.
Screening and application of yeast with high ethanol yield and low fusel oil yield in Chinese Maotai-flavor liquor production
InactiveCN102226155AReduce manufacturing costSimple production processFungiMicrobiological testing/measurementDistillationScreening method
Screening and application of yeast with high ethanol yield and low fusel oil yield in Chinese Maotai-flavor liquor production belong to the technical field of biological engineering. The strain is any one strain selected from pichia anomala CGMCC4740, schizosaccharomyces pombe CGMCC4744, zygosaccharomyces bailii CGMCC4745, trichosporon asahii CGMCC4746, saccharomyces cerevisiae CGMCC4747, kazachstania exigua CGMCC4748, pichia fermentans CGMCC4750. The strain is separated from various Daqu, fermented grains, brewing raw materials and brewing environment of several Chinese famous distilleries; the screening method comprises the following steps: diluting distiller yeast samples, fermented grains, brewing raw materials or brewing environment substances, coating on a TTC primary screening plate, selecting a colony with an obvious red color, inoculating the colony in a shake flask for culture, performing secondary screening, determining the contents of ethanol and fusel oil of the fermentation broth by a distillation specific gravity method and HS-SPME-GC-MS respectively, screening the strain with high ethanol concentration and low fusel oil concentration. The strain is applicable to food industry and brewed wine industry.
Owner:JIANGNAN UNIV
Production method and feed for health-care Chinese herbal medicine eggs
The invention relates to a production method and feed for health-care Chinese herbal medicine eggs. The feed is prepared by the following method: adding herba leonuri, fructus ligustri lucidi, honeysuckle, dandelion, fructus lycii, setose thistle, serissa serissoides, angelica sinensis, and rhizoma zingiberis, as well as water for boiling with strong fire, preserving heat with soft fire for 1-2h, cooling and filtering, collecting filtrate, adding white sugar and baeyer zygosaccharomyces bailii strain for fermentation to obtain fermentation liquor, adding corn, bran and bean pulp into the fermentation liquor, and directly feeding the broiler chicken so as to improve the immunity function of local chicken and prevent the occurrence of diseases. The production method for health-care Chinese herbal medicine eggs does not use antibiotics in the whole feeding process, has no harm to the chicken and is green and environment-friendly, pollution-free and purely zoological, and the production method has the advantages that the eggs have no residual of antibiotics and hormone, thereby enabling people to safely eat and being beneficial to the health of people; in addition, the effective constituent content of the Chinese herbal medicine can be multiplied after being subjected to microbial fermentation, so that the Chinese herbal medicine has the functions of replenishing blood, preventing and treating diseases, as well as the functions of dietetic invigoration and herbal nourishment.
Owner:安徽惠尔普生物科技发展有限责任公司
Aldehyde ketoreductase bacterial strain, aldehyde ketoreductase gene, vector, engineering bacteria and application thereof
InactiveCN104498510AHeight difference selectivityHigh extraction yieldFungiBacteriaNucleotideBacterial strain
The invention discloses aldehyde ketoreductase bacterial strain, aldehyde ketoreductase gene, a vector, engineering bacteria and an application thereof. A nucleotide sequence of aldehyde ketoreductase gene is one of a SEQ ID No. 1, a SEQ ID No. 3 and a SEQ ID No. 5, aldehyde ketoreductase gene shown by the SEQ ID No. 1 and the SEQ ID No. 3 comes from bacterial strain-kluyveromyces lactis XP1461, and aldehyde ketoreductase gene shown by the SEQ ID No. 5 comes from bacterial strain-baeyer zygosaccharomyces bailii XP1462. The disclosed aldehyde ketoreductase from K.lactis and Z. bailii can be firstly reported for biological catalyzed synthesis of 6-cyan-(3R, 5R)-dihydroxyhexanoate, optical voidness of the prepared sample 6-cyan-(3R, 5R)-dihydroxyhexanoate can be reached, existence of 6-cyan-(3R, 5R)-dihydroxyhexanoate can be hardly detected; high epimerase selectivity of aldehyde ketoreductase brings benefit, and the products extraction yield is high.
Owner:ZHEJIANG UNIV OF TECH
Traditional Chinese medicine microbial ecological agent for preventing and treating infectious bursal disease of chicken and preparation method thereof
InactiveCN103961418AIncrease the number ofImprove disease resistanceAntiviralsPlant ingredientsBiotechnologyDisease
Owner:广州中冠动物药业有限公司
Chinese medicinal formulation for treating cardiovascular and cerebrovascular diseases and preparation process of products thereof
InactiveCN102225100AQuick treatmentNo repetitionAnthropod material medical ingredientsCardiovascular disorderSequelaDisease
The invention discloses a Chinese medicinal formulation for treating cardiovascular and cerebrovascular diseases and a preparation process of products thereof. The preparation process comprises the following steps of: decocting Chinese angelica, chuanxiong rhizome, safflower, salvia miltiorrhiza, American ginseng, notoginseng, tree peony bark, earthworms, leech and gradfly; filtering; adding Zygosaccharomyces bailii to the resulting filtrate and filter residues, standing to culture in appropriate temperature and humidity environments for 168 to 240 hours, and making oral liquids, oral tablets or capsules. The main indications of the product disclosed by the invention include cardiovascular and cerebrovascular diseases, hypertension and blood diseases, and the product is directly taken, has rapid onset of action, prevents the recurrence of diseases, is absorbed rapidly, has high curative effects, is sweet in taste, and cause no sequela or complication after administration. By applying microorganisms to Chinese medicines, the two special processes of solid fermentation and liquid fermentation are achieved, so as to eliminate the toxic and adverse effects of active pharmaceutical ingredients, provide convenience for storage and administration and not produce wastes or residues. Besides, the resources of raw materials are abundant, the production is simple and low in cost, and the product is applied to both therapy and health care.
Owner:李现华
A Qi and blood tonifying functional beverage
InactiveCN104997087AImprove immunityImprove drug dissolutionMetabolism disorderDigestive systemBiotechnologyFermentation
The present invention discloses a Qi and blood tonifying functional beverage and a preparation method thereof. The functional beverage consists of the following components in parts by weight: 12-18 parts of donkey-hide gelatin, 8-10 parts of rhizoma polygonati, 12-18 parts of Chinese yam,14-16 parts of Chinese wolfberry fruits, 8-12 parts of mulberries, 50-70 parts of honey, 50-70 parts of white granulated sugar and 5,000-6,000 parts of water. Through fermenting ingredients of a reasonable medicinal formula and breaking wall by fermentation, the drug effect is enhanced, and through a combined fermentation of zygosaccharomyces bailii and bacillus xylinus, a variety of vitamins, amino acids, trace elements and ingredients beneficial to human body contained in raw materials are dissolved out without adding any hormones or chemical compositions, thus the Qi and blood tonifying effect is achieved, and effects in physical health, strengthening body, and enhancing body immunity are also achieved after a user drinks the functional beverage. The functional beverage is suitable for both males and females, and the promotion of the functional beverage benefits the nation and the people.
Owner:杜圣艳
Method for preparing biological agent by radix euphorbiae ebracteolatae
InactiveCN103918723ALow toxicityGood effectBiocideMicroorganism based processesCuticlePesticide residue
The invention discloses a method for preparing a biological agent by radix euphorbiae ebracteolatae. The method comprises the following steps: washing off sundries on the skin of the collected radix euphorbiae ebracteolatae, and naturally airing for 2-3 days at the temperature of 15-25 DEG C; smashing processed raw materials in the first step, putting the smashed raw materials in a fermentation chamber, slowly adding sweet water and stirring until the ratio of material to liquid is 1:(0.8-1), then adding zygosaccharomyces bailii and acetobacter xylinum being 0.1-0.5% of the material and liquid total quantity, wherein the mixing ratio of the zygosaccharomyces bailii to the acetobacter xylinum is 1:(0.8-1), and fermenting for 3-6 days at a constant temperature; taking fermentation liquid on the upper layer in the second step, and after filtering and removing particles in the fermentation liquid, combining the fermentation liquid, auxiliaries and water in proportions to prepare the biological agent. The zygosaccharomyces bailii and the acetobacter xylinum are used for fermenting the radix euphorbiae ebracteolatae, so that radix euphorbiae ebracteolatae ponicidin is greatly analyzed, and meanwhile the toxicity of radix euphorbiae ebracteolatae is eliminated; the prepared biological agent has remarkable effects on disease control of wheat, rice, apples and cucumbers, and is free of pesticide residues.
Owner:安徽省淮北市农药厂
Production method and feedstuff of Chinese herbal medicinal food ducks
InactiveCN102972343AIncrease contentChange the tasteFood processingAnimal feeding stuffSweet flavorRoast duck
The invention discloses a production method and feedstuff of Chinese herbal medicinal food ducks. The feedstuff is produced according to the following method: boiling rhizoma atractylodis, witloof, pericarpium citri reticulatae, serissa serissoides, angelica sinensis, codonopsis pilosula, poria cocos, herba epimedii, and fennel in water with strong fire and then preserving heat for 1-2 hours with soft fire; cooling, filtering and collecting the filtrate; adding white sugar and zygosaccharomyces bailii to obtain fermentation broth after being fermented; and adding corn, bran and bean pulp into the fermentation broth. The feedstuff can be directly fed to ducks, and can improve the immune function of the ducks and prevent diseases. In the production method of the Chinese herbal medicinal food ducks, no antibiotic is used in the entire feeding process of the ducks, no antibiotic or hormone is remnant in the duck products, thereby being safe to the huamn body of an eater, and being beneficial to the health of the body. The Chinese herbs are fed to the ducks after fermentation, so that the content of effective constituents of the Chinese herbs is increased in fold, the sweet flavor permeates into the duck meat, and the amino acid content in the duck meat is increased. Therefore, the taste of the duck meat is changed, the duck meat is stronger in sweet flavor, and the conventional roast ducks, boiled salted ducks and aromatic ducks are changed. Meanwhile, the Chinese herbal medicinal food ducks have the functions of replenishing blood and preventing and curing diseases, and have both dietetic invigoration effect and pharmaceutical invigoration effect.
Owner:安徽惠尔普生物科技发展有限责任公司
Primer and probe for Zygosaccharomyces bailii detection and detection method thereof
PendingCN105603118AEfficient detectionAccurate identificationMicrobiological testing/measurementMicroorganism based processesBiotechnologyFruit juice
The invention relates to a quick qualitative detection technology on Zygosaccharomyces bailii in grape wine and fruit juice and in particular relates to a primer and a probe which are used for Zygosaccharomyces bailii detection and a detection method thereof. According to the invention, a 26S ribosome RNA gene sequence of Zygosaccharomyces bailii is selected, a specific primer and probe set is designed, and simple and quick detection on Zygosaccharomyces bailii in the grape wine and fruit juice can be realized by adopting the primer and the probe and a real-time fluorescent PCR technology.
Owner:INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Preparation method of antioxidant-rich Kombucha tea
InactiveCN110150420AImprove antioxidant capacityShorten the fermentation cycleFungiBacteriaBlack teaPhenol
The invention discloses a preparation method of antioxidant-rich Kombucha tea. The preparation method comprises the following steps of mixing and boiling water, cane sugar and black tea leaves according to the weight ratio of (125-130) to (12.5-13) to (1-1.5), wherein the obtained liquid is a base liquid; inoculating the base liquid into a seed solution prepared by mixing an acetobacter pasteurianus seed solution with the concentration of 105-107 CFU / mL, an acetobacter xylinum seed solution with the concentration of 106-108 CFU / mL and Zygosaccharomyces bailii with the concentration of 105-107CFU / mL according to the volume ratio of 1 to 1 to 1 to obtain a solution to be fermented, and the volume ratio of the mixed seed solution to the base liquid is (3-3.5) to (97-97.5); placing the solution to be fermented in a container with a gauze covered at an open end, and executing standing and fermentation under the conditions of 30%-40% humidity and 25-30 DEG C for 10 days to obtain the Kombucha tea. The fermentation period of the Kombucha tea is shortened, the content of total flavones and total phenols in the obtained Kombucha tea is high, and the oxidation resistance is strong.
Owner:DALIAN POLYTECHNIC UNIVERSITY
Quantitative analysis method of Zygosaccharomyces bailii
PendingCN111560455AStrong specificityStable amplificationMicrobiological testing/measurementMicroorganism based processesBiotechnologyYeast
The invention relates to the technical field of white spirit brewing, in particular to a quantitative analysis method of important saccharomycetes Zygosaccharomyces bailii in a white spirit solid-state fermentation process. According to the invention, a specific primer is adopted to carry out quantitative PCR analysis on 26S rRNA of Zygosaccharomyces bailii, so as to analyze the variation trend ofZygosaccharomyces bailii in Daqu and fermented grains and predict the alcohol yield. The method disclosed by the invention has the advantages of high detection speed, high sensitivity, good accuracy,good specificity and no culture.
Owner:KWEICHOW MOUTAI COMPANY
Screening and application of yeast CGMCC 4745 capable of high yield of ethanol and low yield of fusel oil in production of Chinese Maotai-flavor liquor
InactiveCN102816706AReduce manufacturing costSimple production processFungiAlcoholic beverage preparationHigh concentrationDistillation
The invention provides screening and application of the yeast CGMCC 4745 capable of high yield of ethanol and low yield of fusel oil in production of Chinese Maotai-flavor liquor, which belongs to the technical field of bioengineering. A bacterial strain used in the invention is Zygosaccharomyces bailii CGMCC 4745. The bacterial strain is separated from a variety of Daqu, fermented grains, brewage raw materials and brewing environment materials in a plurality of famous distilleries in China. The screening method for the bacterial strain comprises the following steps: taking and diluting a Daqu sample, fermented grains, brewage raw materials or brewing environment materials, coating diluted substances on a TTC primary screening plate, choosing a substantially red bacterium colony, inoculating the substantially red bacterium colony into a shake flask for culture, carrying out secondary screening, determining the content of ethanol and fusel oil in obtained fermentation broth by respectively using a distillation specific gravity method and HS-SPME-GC-MS and screening out a bacterial strain with high concentration of ethanol and low concentration of fusel oil. The bacterial strain can be used in the industries of food and wine making.
Owner:JIANGNAN UNIV
Clinical mixed preparation for cardiovascular department and preparation method
InactiveCN105456390AQuick treatmentNo repetitionOrganic active ingredientsAnthropod material medical ingredientsSalvia miltiorrhizaSequela
The invention discloses a clinical mixed preparation for a cardiovascular department and a preparation method, and belongs to the field of traditional Chinese medicine. The effective component of the clinical mixed preparation is prepared through the steps that radix angelica sinensis, ligusticum wallichii, safflower carthamus, salvia miltiorrhiza, American ginseng, panax notoginseng, cortex moutan radicis, lumbricus, leeches and gadflies are decocted and then filtered, zygosaccharomyces bailii is added in obtained filtrate and medicine dregs, and the zygosaccharomyces bailii is cultured for 168-240 hours in a standing mode in a corresponding temperature and humidity environment to obtain oral liquid or orally-taken tablets or capsules. The clinical mixed preparation mainly treats heart and cerebral vusculer diseases, hypertension and blood diseases, is directly and orally taken and is capable of quickly achieving the effects, free of relapse, rapid to absorb, high in treating effect and sweet in taste, and no sequela or complication occurs after the clinical mixed preparation is taken; two special technologies of solid fermentation and liquid fermentation are achieved in the traditional Chinese medicine through microorganism application, the toxic and side effects of the raw material medicine are removed, storing and eating are convenient, and no waste medicine dreg is generated. The clinical mixed preparation is rich in raw material source, easy to prepare, low in cost, capable of achieving both treatment and health care and suitable for treating, rehabilitating and nursing of cardiovascular department diseases.
Owner:夏修菲
Method for preparing bactericide from bran
InactiveCN103918725ANo pesticide residueSimple preparation processBiocideFungicidesApigeninPesticide residue
The invention discloses a method for preparing a bactericide from bran, which comprises the following steps: dissolving sugar in water to form a sugar solution; mixing Saturnospora dispora, Zygosaccharomyces bailii and Acetobacter xylinum to form a mixed bacterium solution; mixing the sugar solution and bacterium solution to form a mixed solution, and putting the bran into a fermenting tank layer by layer, wherein the mixed solution is uniformly sprayed on each layer until the layer is moistened; fermenting at constant temperature, filtering the fermented liquor to obtain fermentation liquor and solid residues, adding sodium hydroxide into the obtained fermentation liquor to regulate the pH value, and filtering to obtain a mixture containing apigenin (belonging to flavones), 5-nonadecyl resorcinol, 5-heneicosyl resorcinol and 5-tricosyl resorcinol (belonging to alkyl resorcinols), and pentosan, chitosan, oligosaccharide, amino oligosaccharide, lentinan and pujuxitang; and preparing the plant-source agricultural bactericide. The preparation technique is simple and suitable for large-scale production; and the plant-source agricultural bactericide has the advantages of high safety, environmental protection and no pesticide residue.
Owner:安徽省淮北市农药厂
Antimicrobial combinations of sorbate, natamycin and dialkyl dicarbonate for use in food products
Antimicrobial combinations comprising a sorbate preservative, natamycin and a dialkyl dicarbonate at levels below the taste threshold for each of these antimicrobials but such that combination is effective against food spoilage microorganisms. These antimicrobial combinations are useful in treating beverages, especially dilute juice beverages, calcium fortified beverages, beverages containing tea solids, and beverages containing milk solids and proteins, as well as other acidified, high water activity food and beverage products such as cheese, sausage, ready-to-spread frostings, salad dressings, mayonnaise, and the like, that are susceptible to food spoilage microorganisms, including yeasts such as Zygosaccharomyces bailii.
Owner:PROCTER & GAMBLE CO
Production method and feed for Chinese herbal medicine chicken
InactiveCN102972647BGood for healthIncrease contentFood processingAnimal feeding stuffDiseaseRHODIOLA ROSEA ROOT
The invention relates to a production method and feed for Chinese herbal medicine chicken. The feed is prepared by the following method: adding rhodiola rosea, rhizoma chuanxiong, phyllanthus ruinaria, fructus aurantii, common scouring rush herb, lysimachia christinae, dried tangerine peel, cichory, angelica sinensis, and plantain herb, as well as water for boiling with strong fire, preserving heat with soft fire for 1-2h, cooling and filtering, collecting filtrate, adding white sugar and baeyer zygosaccharomyces bailii strain for fermentation to obtain fermentation liquor, adding corn, bran and bean pulp into the fermentation liquor, and directly feeding the local chicken so as to improve the immunity function of local chicken and prevent the occurrence of diseases. The production method for Chinese herbal medicine broiler chicken does not use antibiotics in the whole feeding process, has no harm to the chicken and is green and environment-friendly, pollution-free and purely zoological, and the production method has the advantages that the chicken and eggs have no residual of antibiotics and hormone, thereby enabling people to safely eat and being beneficial to the health of people; in addition, the effective constituent content of the Chinese herbal medicine can be multiplied after being subjected to microbial fermentation, so that the Chinese herbal medicine has the functions of replenishing blood, preventing and treating diseases, as well as the functions of dietetic invigoration and herbal nourishment.
Owner:安徽惠尔普生物科技发展有限责任公司
Method for preparing biological reagent with melia azedarach fruits
The invention discloses a method for preparing a biological reagent with melia azedarach fruits. The method comprises the following steps: firstly, treating raw materials, namely, cleaning collected melia azedarach fruits to remove sundries on the surfaces, and airing for later use; secondly, carrying out fermentation detoxication, namely, putting the treated raw materials in the first step into a fermenting chamber, slowly adding sweet water and stirring so as to enable the ratio of materials to liquid is 1:(1.2-1.5), then adding zygosaccharomyces bailii and acetobacter xylinum with the material and liquid total quantity of 0.1-0.5% until the mixing ratio of the Bayer combined saccharomycetes to the acetobacter xylinum to be 1:(0.8-1), keeping the temperature to be 26-30 DEG C, and fermenting for 3-6 days; and thirdly, preparing the biological reagent, namely, filtering and removing particles in the fermentation liquid taken on the upper layer in the second step, and combining the fermentation liquid, auxiliaries and water in proportions to prepare the biological reagent. The zygosaccharomyces bailii and the acetobacter xylinum are used for fermenting the melia azedarach fruits, so that melia azedarach alkali is greatly analyzed; the prepared biological reagent can be taken as a broad-spectrum insecticidal bacterium reagent, and is free of residues.
Owner:安徽省淮北市农药厂
Method for preparing bactericide from rice hulls
The invention discloses a method for preparing a bactericide from rice hulls, which comprises the following steps: uniformly mixing bran and rice hulls, and dissolving sugar in water to form a sugar solution; mixing Saturnospora dispora, Zygosaccharomyces bailii and Acetobacter xylinum; putting the mixture into a fermenting tank layer by layer, wherein the mixed solution of the sugar solution and mixed bacterium solution is uniformly sprayed on each layer until the layer is moistened; fermenting at constant temperature, filtering the fermented liquor to obtain fermentation liquor and solid residues, adding sodium hydroxide into the obtained fermentation liquor to regulate the pH value, and filtering to obtain a mixture containing apigenin (belonging to flavones), 5-nonadecyl resorcinol, 5-heneicosyl resorcinol and 5-tricosyl resorcinol (belonging to alkyl resorcinols), and pentosan, chitosan, oligosaccharide, amino oligosaccharide, lentinan and pujuxitang; and preparing the plant-source agricultural bactericide by using the mixture. The preparation technique is simple and suitable for large-scale production; and the plant-source agricultural bactericide has the advantages of high safety, environmental protection and no pesticide residue.
Owner:安徽省淮北市农药厂
Production method and feed for health-care Chinese herbal medicine eggs
InactiveCN102972646BGood for healthIncrease contentAnimal feeding stuffDiseaseAngelica Sinensis Root
Owner:安徽惠尔普生物科技发展有限责任公司
Method for preparing fungicide from bran
InactiveCN103918725BNo pesticide residueSimple preparation processBiocideFungicidesApigeninPesticide residue
Owner:安徽省淮北市农药厂
Zygosaccharomyces bayeri strain used for prevention and treatment of postharvest fruit diseases and its preparation method and application
ActiveCN107937290BBroad antibacterial spectrumPromote growthFungiFruit and vegetables preservationBiotechnologyFragaria
The invention discloses a strain of Zygosaccharomyces bailii BY39 strain with wide antibacterial spectrum and stable effect for the prevention and treatment of postharvest fruit diseases, as well as its preparation method and application. The preservation strain number of the strain in the General Microorganism Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.14912. The method of using the Zygosaccharomyces Bayer yeast BY39: activate the strain, ferment and cultivate it with YPD, centrifuge, and use sterile water to make 1×10 8 cells / mL of bacterial suspension; put apples, grapes, strawberries, citrus or cherry tomatoes into the bacterial suspension, soak for 30 seconds, take out, and air-dry; put them in a fresh-keeping box and store at room temperature. This wine Saccharomyces cerevisiae strain simultaneously controls apple black spot, grape cinerea, aspergillosis, and fusarium, strawberry botrytis and aspergillosis, citrus penicillium, and cherry tomato botrytis and aspergillosis, It can reduce the losses caused by postharvest diseases and has a good application prospect.
Owner:南京万拓生物科技有限公司
Absolute quantification probe and method for zygosaccharomyces bayer and application of probe and method
ActiveCN112924428ARealize the total detectionImprove accuracyMicrobiological testing/measurementFluorescence/phosphorescenceZygosaccharomyces bailiiZygosaccharomyces
The invention discloses a probe and a method for absolute quantification of zygosaccharomyces bayer and application of the probe and the method, and belongs to the fields of biology, fermentation and detection. According to the zygosaccharomyces bayer quantitative probe and the kit, total quantity detection of zygosaccharomyces bayer can be achieved, expensive instruments are not needed when the zygosaccharomyces bayer quantitative probe and the kit are used for detection and zygosaccharomyces bayer quantification, and quantification work can be rapidly completed within 2.5 h. Meanwhile, nucleic acid extraction does not need to be carried out on a sample used in the method. Based on the probe and the detection kit provided by the invention, the detection kit is used for quantifying the zygosaccharomyces bayer and has the characteristics of rapidness, convenience, cheapness and accuracy.
Owner:JIANGNAN UNIV
Method for preparing fungicide by using bran and rice husk
The invention discloses a method for preparing a bactericide from rice hulls, which comprises the following steps: uniformly mixing bran and rice hulls, and dissolving sugar in water to form a sugar solution; mixing Saturnospora dispora, Zygosaccharomyces bailii and Acetobacter xylinum; putting the mixture into a fermenting tank layer by layer, wherein the mixed solution of the sugar solution and mixed bacterium solution is uniformly sprayed on each layer until the layer is moistened; fermenting at constant temperature, filtering the fermented liquor to obtain fermentation liquor and solid residues, adding sodium hydroxide into the obtained fermentation liquor to regulate the pH value, and filtering to obtain a mixture containing apigenin (belonging to flavones), 5-nonadecyl resorcinol, 5-heneicosyl resorcinol and 5-tricosyl resorcinol (belonging to alkyl resorcinols), and pentosan, chitosan, oligosaccharide, amino oligosaccharide, lentinan and pujuxitang; and preparing the plant-source agricultural bactericide by using the mixture. The preparation technique is simple and suitable for large-scale production; and the plant-source agricultural bactericide has the advantages of high safety, environmental protection and no pesticide residue.
Owner:安徽省淮北市农药厂
Traditional Chinese medicine microecologics preparation for preventing and treating pullorum disease and preparation method of microecologics preparation
ActiveCN103495007BEnhance immune functionNo side effectsAntibacterial agentsBacteria material medical ingredientsSide effectGLYCYRRHIZA EXTRACT
The invention discloses a traditional Chinese medicine microecologics preparation for preventing and treating pullorum diseases, and a preparation method of the microecologics preparation. The microecologics preparation is prepared by boiling the root of Chinese pulsatilla, honeysuckle, garlic, coptis chinensis, scutellaria baicalensis, golden cypress, hawthorn, dried orange peels, medicated leaven and liquorice in water through strong fire, keeping the temperature through soft fire for 30-60 minutes, cooling and filtering, collecting the filtrate, adding white sugar to boil, cooling to the normal temperature, subsequently inoculating lactobacillus, zygosaccharomyces bailii and acetobacter xylinum, and fermenting for 3-7 days so as to obtain the microecologics preparation, and the prepared microecologics preparation is diluted according to ratios and is taken by chicken directly. The traditional Chinese medicine microecologics preparation disclosed by the invention has the effects of clearing heat and detoxifying, strengthening the spleen and stomach, cooling blood and treating dysentery, and is simple in preparation method, convenient to use, free of medicine residue, free of toxic and side effect and high in viable bacteria content. The traditional Chinese medicine microecologics preparation disclosed by the invention is applicable to demands of large-scale industrial production, and has a good market prospect.
Owner:广州市中添生物科技有限公司
A probe, method and application for absolute quantification of Zygomyces bayeri
ActiveCN112924428BRealize the total detectionImprove accuracyMicrobiological testing/measurementMaterial analysis by optical meansBiotechnologyZygosaccharomyces bailii
The invention discloses a probe, method and application for absolute quantification of Zygomyces zygosacrum Bayer, and belongs to the fields of biology, fermentation and detection. The Zygomycetes quantitative probe and kit of the present invention can realize the detection of the total amount of Zygomycetes Bayer, do not need to use expensive instruments when used for detection and quantification of Zygomycete Bayer, and can quickly complete the quantitative work within 2.5 hours. At the same time, the samples used in the present invention do not necessarily have to undergo nucleic acid extraction. The probe and detection kit based on the present invention are used for the quantification of Zygomycetes Bayer, and have the characteristics of rapidity, convenience, cheapness and accuracy.
Owner:JIANGNAN UNIV
A traditional Chinese medicine microecological preparation for preventing and treating chicken infectious bursal disease and its preparation method
InactiveCN103961418BIncrease the number ofImprove disease resistanceAntiviralsPlant ingredientsBiotechnologyDisease
The invention provides a traditional Chinese medicine microbial ecological agent for preventing and treating infectious bursal disease of chicken and a preparation method thereof. The preparation method comprises the following steps: adding water to radix isatidis, honeysuckle, forsythia, folium isatidis, humulus scandens, radix scutellariae, dangshen, ageratum, plantain, astragalus membranaceus, angelica sinensis and liquorice, boiling by using high heat, preserving heat for 30-60 minutes by using slow fire, cooling, filtering, collecting filtrate, adding white sugar, boiling, cooling to a normal temperature, then inoculating lactobacillus, zygosaccharomyces bailii and acetobacter xylinum, and fermenting for 3-7 days so as to obtain the microbial ecological agent. After the microbial ecological agent is diluted proportionally, the diluted microbial ecological agent is directly drunk by chicken. The traditional Chinese medicine microbial ecological agent provided by the invention has the effects of resisting viruses, inducing interferon, increasing the quantity of effective microbial communities in intestinal tracts, boosting immunity and improving disease resistance of chickens without toxic or side effects and the like; the preparation method is simple, convenient to use and high in viable bacteria content and has no residues. The traditional Chinese medicine microbial ecological agent provided by the invention has a certain curative effect on infectious bronchitis and henpox and relatively good market prospect.
Owner:广州中冠动物药业有限公司
Screening and application of yeast with high ethanol yield and low fusel oil yield in Chinese Maotai-flavor liquor production
InactiveCN102226155BReduce manufacturing costSimple production processFungiMicrobiological testing/measurementScreening methodBio engineering
Owner:JIANGNAN UNIV
Environmental microorganism zygosaccharomyces bailii
InactiveCN105238706AImprove pollutionImprove controlFungiMicroorganism based processesYeastMicroorganism
The invention discloses environmental microorganism zygosaccharomyces bailii. The environmental microorganism zygosaccharomyces bailii, which is discovered in a process of producing strong loquat syrup, is named zygosaccharomyces bailii HQ, and the environmental microorganism zygosaccharomyces bailii is preserved in China Center for Type Culture Collection on October 26, 2015 with preservation number of CCTCC NO: M2015641, in Wuhan University, Wuchang District, Wuhan, Hubei Province, postcode: 430072. The discovered novel pollutant strain is helpful for pharmaceutical enterprises to take an active effect in fights of attacking and defending pollution of the bacterium, so that microorganism pollution monitoring and controlling capacities are significantly improved.
Owner:HANGZHOU HUQINGYUTANG PHARM CO LTD
Screening and application of yeast CGMCC 4745 capable of high yield of ethanol and low yield of fusel oil in production of Chinese Maotai-flavor liquor
InactiveCN102816706BReduce manufacturing costSimple production processFungiMicroorganism based processesHigh concentrationDistillation
The invention provides screening and application of the yeast CGMCC 4745 capable of high yield of ethanol and low yield of fusel oil in production of Chinese Maotai-flavor liquor, which belongs to the technical field of bioengineering. A bacterial strain used in the invention is Zygosaccharomyces bailii CGMCC 4745. The bacterial strain is separated from a variety of Daqu, fermented grains, brewage raw materials and brewing environment materials in a plurality of famous distilleries in China. The screening method for the bacterial strain comprises the following steps: taking and diluting a Daqu sample, fermented grains, brewage raw materials or brewing environment materials, coating diluted substances on a TTC primary screening plate, choosing a substantially red bacterium colony, inoculating the substantially red bacterium colony into a shake flask for culture, carrying out secondary screening, determining the content of ethanol and fusel oil in obtained fermentation broth by respectively using a distillation specific gravity method and HS-SPME-GC-MS and screening out a bacterial strain with high concentration of ethanol and low concentration of fusel oil. The bacterial strain can be used in the industries of food and wine making.
Owner:JIANGNAN UNIV
Functional beverage capable of decreasing blood sugar
InactiveCN104856046AEliminate side effectsImprove immunityFood ingredient functionsFood preparationBiotechnologyOfficinalis
The invention discloses a functional beverage capable of decreasing blood sugar. The functional beverage is prepared from the following raw materials in parts by weight: 12-18 parts of Chinese yam, 12-18 parts of radix polygonati officinalis, 8-10 parts of fructus gardenia, 8-10 parts of radix puerariae, 14-17 parts of lily, 8-12 parts of dark plum, 120-150 parts of honey and 9000-11000 parts of water. The invention further discloses a preparation method of the functional beverage. By virtue of a reasonable composition, the pure natural traditional Chinese medicine functional beverage is provided; after the traditional Chinese medicines are fermented, toxic and side effects of the traditional Chinese medicines are eliminated; by virtue of fermentation and wall breakage, the dissolution of pesticide effect is improved; furthermore, through fermentation through zygosaccharomyces bailii and acetobacter xylinum, various vitamins, amino acids and microelements contained in the raw materials are dissolved out, and no hormone and chemical components are added, so that the functional beverage has the effect of preventing and treating blood sugar diseases after being drunk as well as efficacies of building bodies and enhancing the immunity of the organism. By virtue of the popularization of the functional beverage, the nation and the people are benefited.
Owner:杜圣艳
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