Zygosaccharomyces bayeri strain used for prevention and treatment of postharvest fruit diseases and its preparation method and application

A technology for Zygomyces zygosaccharomyces and fruit postharvest diseases, applied in the direction of microbial-based methods, biochemical equipment and methods, and preservation of fruits and vegetables, which can solve the lack of antibacterial spectrum strains, and the biocontrol effect has only been verified on a small number of fruits To achieve significant social and ecological benefits, avoid harm to people, and grow well

Active Publication Date: 2021-01-19
南京万拓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

Method used

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  • Zygosaccharomyces bayeri strain used for prevention and treatment of postharvest fruit diseases and its preparation method and application
  • Zygosaccharomyces bayeri strain used for prevention and treatment of postharvest fruit diseases and its preparation method and application
  • Zygosaccharomyces bayeri strain used for prevention and treatment of postharvest fruit diseases and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Biological properties of Zygosaccharomyces bailii strain BY39

[0020] 1. Morphological features

[0021] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.

[0022] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.

[0023] 2. Molecular biological identification

[0024] Use the universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and the reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') to PCR amplify the yeast 26S rDNA D1 / D2 region nucleic acid sequence, and PCR The sequencing results of the product were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences were downloaded from the GenBank...

Embodiment 2

[0026] The inhibitory effect of embodiment 2 Zygosaccharomyces Bayer BY39 on apple black spot

[0027] 1. Experimental protocol

[0028] Take Zygmus yeast BY39 out of the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 Cells / mL of Zygomyces Bayeris BY39 suspension.

[0029] Alternaria tenuissima was activated on a PDA medium plate, cultured at 26°C for 7-14 days, scraped an appropriate amount of spores, and prepared with sterile water to a concentration of 5×10 4 cells / mL of Alternaria spore suspension.

[0030] Healthy and undamaged apple fruits were steril...

Embodiment 3

[0033] Example 3 Inhibitory Effect of Zygosaccharomyces Bayer BY39 on Grape Postharvest Diseases

[0034] 1. Experimental protocol

[0035] Take Zygmus yeast BY39 out of the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 cells / mL of Zyggiospermia BY39 suspension.

[0036] Activate Botrytis porri, Aspergillus aculeatus or Fusarium redolens on PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare with sterile water into a concentration of 5 x 10 4 cells / mL of spore suspension of Botrytis cinerea, Aspergillus thornsp...

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Abstract

The invention discloses a strain of Zygosaccharomyces bailii BY39 strain with wide antibacterial spectrum and stable effect for the prevention and treatment of postharvest fruit diseases, as well as its preparation method and application. The preservation strain number of the strain in the General Microorganism Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.14912. The method of using the Zygosaccharomyces Bayer yeast BY39: activate the strain, ferment and cultivate it with YPD, centrifuge, and use sterile water to make 1×10 8 cells / mL of bacterial suspension; put apples, grapes, strawberries, citrus or cherry tomatoes into the bacterial suspension, soak for 30 seconds, take out, and air-dry; put them in a fresh-keeping box and store at room temperature. This wine Saccharomyces cerevisiae strain simultaneously controls apple black spot, grape cinerea, aspergillosis, and fusarium, strawberry botrytis and aspergillosis, citrus penicillium, and cherry tomato botrytis and aspergillosis, It can reduce the losses caused by postharvest diseases and has a good application prospect.

Description

technical field [0001] The invention relates to the field of biological control of postharvest diseases of fruits, in particular to a strain of Zygosaccharomyces bailii used for biological control of postharvest diseases of fruits. Diseases have a significant control effect. Background technique [0002] Although the quality deterioration of fresh fruits and vegetables is affected by many factors, disease is the main reason. Among them, rot and deterioration caused by fungal diseases is the most serious factor in postharvest fruit loss. Although postharvest diseases of fruits and vegetables can be prevented and controlled in many ways such as agricultural control, physical control, chemical control and biological control, the main measure at present is chemical control (Eckert & Ogawa, 1985, 1988). However, the long-term use of chemical pesticides not only leads to the development of resistance of pathogenic bacteria and reduces the bactericidal effect (Prusky et al., 1985...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23B7/155C12R1/645
CPCA23B7/155C12N1/16C12N1/145C12R2001/645
Inventor 王友升姚婷马琳黄津津
Owner 南京万拓生物科技有限公司
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