Ultra performance liquid chromatography method for simultaneously determining contents of uridine, guanosine and adenosine in rhizoma pinelliae extract

An ultra-high performance liquid phase, adenosine content technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of long time and poor separation effect.

Active Publication Date: 2014-07-16
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
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Problems solved by technology

The currently reported determination of the nucleoside components of Pinellia medicinal materials and decoction pieces all us...

Method used

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  • Ultra performance liquid chromatography method for simultaneously determining contents of uridine, guanosine and adenosine in rhizoma pinelliae extract

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of the test solution: 80g of pinellia medicinal material was crushed into a coarse powder, and 6.8g of dried ginger was added, crushed into a coarse powder, added 6 times the amount of water, mixed evenly, pressed into a diaphragm filter press, and extracted under pressure at 55°C Twice, 30 minutes each time, filter, combine the filtrates, concentrate to a relative density of 1.10-1.20 (50°C), add 2 times the amount of ethanol to precipitate, filter, recover ethanol from the filtrate, and concentrate to 40ml. Each ml of extract is equivalent to 2g of the original medicinal material. Accurately draw 1ml of the concentrated solution in a 25ml volumetric flask, dilute with water to constant volume, filter through a 0.22μm microporous membrane and take the subsequent filtrate to obtain the test solution.

[0020] The content determination of three kinds of nucleoside components in Pinellia medicinal material: get need testing solution, press above-mentioned chro...

Embodiment 2

[0022] Preparation of the test solution: 160g of pinellia decoction pieces were crushed into a coarse powder, and 13.6g of dried ginger was added, crushed into a coarse powder, added 6 times the amount of water, mixed well, pressed into a diaphragm filter press, and extracted under pressure at 45°C Two times, each time for 30 minutes, filter, combine the filtrates, concentrate to a relative density of 1.10-1.20 (50°C), add 2.5 times the amount of ethanol to precipitate, filter, recover ethanol from the filtrate, and concentrate to 160ml. Each ml of extract is equivalent to 1g of the original medicinal material. Accurately draw 1ml of the concentrated solution in a 25ml volumetric flask, dilute with water to constant volume, filter through a 0.22μm microporous membrane and take the subsequent filtrate to obtain the test solution.

[0023] Pinellia decoction pieces content determination: get the test solution, according to the above-mentioned chromatographic conditions (the chro...

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Abstract

The invention belongs to the field of traditional Chinese medicine composition analytic determination technology, and discloses a method for determining the contents of uridine, guanosine and adenosine in a rhizoma pinelliae medicinal material and decoction piece extract by a UPLC method. A chromatographic column is ACQUITY UPLC BEH C18 (1.7 [mu]m, 2.1*50 mm), a mobile phase is a water-methanol mixture with different-ratio gradient elution, a column temperature is 30 DEG C, the flow rate is 0.5 mL.min<-1>, and the detection wavelength is 254 nm. Uridine, guanosine and adenosine have good linear relationship in an applicable concentration range. The method is accurate, fast and reliable, and can effectively simultaneously determine the contents of uridine, guanosine and adenosine in the rhizoma pinelliae extract.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to an analysis technique for simultaneously determining uridine, guanosine and adenosine in pinellia medicinal materials and extracts of decoction pieces. Background technique [0002] Pinellia is the dry tuber of Pinellia ternate (thumb.) Breit., a plant of the family Araceae. It has the effects of drying dampness and reducing phlegm, reducing adverse reactions and relieving vomiting, and dispelling swelling and stagnation. It is widely used in clinical practice. Pinellia contains a variety of chemical components, mainly including nucleosides, organic acids, alkaloids and so on. Among them, nucleosides are the basic components of biological cells to maintain life activities. They have various biological activities such as anti-tumor, anti-virus, and gene therapy. Uridine, Guanosine, and Adenosine. According to literature reports, some scholars at home and abroad have used nucleosides as ind...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 张景勍何丹杨林
Owner CHONGQING MEDICAL UNIVERSITY
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