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Strain with high expression of daptomycin and its screening method

A technology of daptomycin and high expression is applied in the field of antibiotic production strains and their screening to achieve the effects of improving production level, improving screening efficiency and reducing blindness

Inactive Publication Date: 2016-03-30
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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Problems solved by technology

[0008] Ultraviolet mutagenesis technology is one of the commonly used methods in the process of microbial strain selection, but there is no report on the use of ultraviolet mutagenesis combined with rifampicin / gentamicin to improve the production level of daptomycin

Method used

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  • Strain with high expression of daptomycin and its screening method
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  • Strain with high expression of daptomycin and its screening method

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Embodiment Construction

[0039] Highly expressing daptomycin strain Ge-27, classification name: Streptomyces roseosporus (Streptomyces roseosporus), preservation date: November 30, 2012, preservation unit: General Microbiology Center (CGMCC) of China Microbial Culture Collection Management Committee, Deposit number: CGMCCNO.6899.

[0040] Strain RG-4 with high expression of daptomycin, classification and designation: Streptomyces roseosporus, preservation date: December 18, 2012, preservation unit: General Microbiology Center (CGMCC) of China Microbial Culture Collection Management Committee, Deposit number: CGMCCNO.7014.

[0041] Strain Ri-5 with high expression of daptomycin, classification name: Streptomyces roseosporus, preservation date: December 18, 2012, preservation unit: General Microbiology Center (CGMCC) of China Microbial Culture Collection Management Committee, Deposit number: CGMCCNO.7015.

[0042] Hereinafter, the present invention will be further described with embodiments in combination wi...

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Abstract

The invention relates to high-expression daptomycin strains and a screening method thereof, and belongs to the field of antibiotic production strains and screening thereof. The name of the classification of the strains is streptomyces roseosporus. The stains are preserved in the general microbiology center of the China Committee for Culture Collection of Microorganisms, with accession numbers being CGMCC NO.6899, NO.7014 and NO.7015. The invention also provides the screening method of the high-expression daptomycin strains. Ultraviolet mutagenesis and antibiotic resistance screening are combined to increase the mutation probability of the strains, so as to obtain the high-expression daptomycin strains. The output of each of the stains is higher than 59.86 mg / L and can reach 79.44 mg / L. The stains and the screening method can rapidly and effectively increase the production level of the daptomycin and have important practical significance.

Description

Technical field [0001] The invention relates to a strain with high expression of daptomycin and a screening method thereof, and belongs to the field of antibiotic production strains and screening. Background technique [0002] Daptomycin is the N-decylphthalide derivative of A21978, which is produced by the metabolism of Streptomyces roseosporus. Daptomycin molecule is composed of 13 amino acid cyclic lipopeptide and ten-carbon fatty acid chain. When A-21978C undergoes deacylation to remove the N-terminal linking group, A-21978C core can be obtained, and the N-terminal of A21978C core is acylated with capric acid to obtain daptomycin (the structural formula is as follows) . [0003] [0004] Daptomycin structure [0005] In the past forty years, in the field of anti-infective drugs, the only microbial drugs that have entered clinical applications representing a new structural category are the oxazolidone antibiotics represented by linezolid and daptomycin as the representative Cy...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12R1/465
Inventor 刘刚刘体颜陈惠鹏邢玉华张部昌
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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