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A method for preparing antioxidants from Schizochytrium algae dregs

A technology of antioxidants and chytrid algae meal, which is applied in the field of preparation of antioxidants, can solve the problems of complex production process routes, high production costs, and poor flavor of antioxidant peptides, and achieve full utilization, prevention of oxidative deterioration, and good water solubility Effect

Active Publication Date: 2017-05-24
GUANGDONG RUNKE BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In summary, the existing methods for preparing antioxidant peptides have the defects of complex production process routes and high production costs
In addition, the prepared antioxidant peptides have poor flavor (especially bitter taste)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] In the present embodiment, the method for preparing antioxidant from Schizochytrium algae dregs comprises the following steps:

[0046] (1) Using the remaining Schizochytrium algae dregs after extracting oil from Schizochytrium algae as a raw material, adding water to soak the Schizochytrium algae dregs to obtain a feed liquid;

[0047] In this step (1), the weight of the added water is 8 times that of Schizochytrium algae meal, and the soaking time is 60 minutes;

[0048] (2) Stir the feed liquid obtained in step (1) evenly and then perform homogenization treatment;

[0049] In this step (2), the homogenization pressure is 10MPa, and the feed liquid temperature is 70°C during homogenization treatment;

[0050] (3) Sterilize the homogenized feed solution (sterilize at 85°C for 30 minutes), then cool it down to 37°C, adjust the pH value of the feed liquid to 7.5, then add protease to hydrolyze while stirring When the degree of protein hydrolysis is 5%, the hydrolyzate ...

Embodiment 2

[0061] In the present embodiment, the method for preparing antioxidant from Schizochytrium algae dregs comprises the following steps:

[0062] (1) Using the remaining Schizochytrium algae dregs after extracting oil from Schizochytrium algae as a raw material, adding water to soak the Schizochytrium algae dregs to obtain a feed liquid;

[0063] In this step (1), the weight of the added water is 12 times that of Schizochytrium algae meal, and the soaking time is 30 minutes;

[0064] (2) Stir the feed liquid obtained in step (1) evenly and then perform homogenization treatment;

[0065] In this step (2), the homogenization pressure is 25MPa, and the temperature of the feed liquid during homogenization treatment is 30°C;

[0066] (3) Sterilize the homogenized feed solution (sterilize at 90°C for 25 minutes), then cool it to 50°C, and adjust the pH value of the feed liquid to 9.5, then add protease and hydrolyze it under stirring Until the degree of protein hydrolysis is 10%, the...

Embodiment 3

[0077] In the present embodiment, the method for preparing antioxidant from Schizochytrium algae dregs comprises the following steps:

[0078] (1) Using the remaining Schizochytrium algae dregs after extracting oil from Schizochytrium algae as a raw material, adding water to soak the Schizochytrium algae dregs to obtain a feed liquid;

[0079] In this step (1), the weight of the water added is 10 times that of Schizochytrium algae meal, and the soaking time is 45 minutes;

[0080] (2) Stir the feed liquid obtained in step (1) evenly and then perform homogenization treatment;

[0081] In this step (2), the homogenization pressure is 20MPa, and the feed liquid temperature is 45°C during homogenization treatment;

[0082] (3) Sterilize the homogenized feed solution (sterilize at 95°C for 15 minutes), then cool it down to 40°C, adjust the pH value of the feed liquid to 8.5, then add protease to hydrolyze while stirring Until the degree of protein hydrolysis is 9.5%, the hydrolyz...

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PUM

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Abstract

The invention discloses a method for preparing an antioxidant by using schizochytrium limacinum algal meal. The method comprises the following steps: (1) adding water to soak schizochytrium limacinum algal meal to obtain material liquid; (2) uniformly stirring the material liquid and carrying out homogenizing treatment; (3) sterilizing the homogenized material liquid and adjusting the pH value to 7.5-9.5, and then adding protease for hydrolyzing until the degree of hydrolysis of protein is 5-10%, and carrying out enzyme deactivation so as to obtain hydrolysate; (4) centrifugally separating the hydrolysate to obtain supernate A; (5) participating the supernate A by using ethyl alcohol, and centrifugally separating to obtain supernate B; (6) removing ethyl alcohol out of the supernate B to obtain supernate C; (7) concentrating the supernate C, then adding glucose and reacting to obtain a Maillard reaction product; (8) carrying out spray drying on the Maillard reaction product to obtain the antioxidant. The schizochytrium limacinum algal meal is taken as a raw material, so that the method is simple in production process and low in production cost, and the prepared antioxidant is high in antioxidant activity and high in eating safety.

Description

technical field [0001] The invention relates to the preparation of peptides, in particular to a method for preparing antioxidants from Schizochytrium algae dregs, that is, a method for preparing antioxidants from the remaining algae dregs after extracting oil from Schizochytrium algae. Background technique [0002] For oily foods, many studies have shown that the deterioration of all oily foods begins with fat oxidation. Fat oxidation methods include air oxidation, photooxidation, enzymatic oxidation, metal-catalyzed oxidation, etc. The oxidized oil has three major harms: one is the irritating smell such as "hala taste". When this happens to infant milk powder, the child's direct performance is refusal to eat, even if it is barely eaten, vomiting will occur; the other is nutrition. The value is destroyed, and unsaturated fatty acids no longer have nutritional value after oxidation; the third is to produce toxic substances, such as hydroperoxides, aldehydes, epoxides, etc. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02
Inventor 李建平任国谱
Owner GUANGDONG RUNKE BIOLOGICAL ENG
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