Liquid hard surface antimicrobial cleaning composition
An anti-microbial and composition technology, applied in the direction of cationic surface active compounds, detergent compositions, organic cleaning compositions, etc., can solve the problems of no disclosure of the anti-microbial effect of active ingredients, no solution to the effect of anti-microbial compositions, etc.
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Embodiment 1 to 3
[0049] Examples 1 to 3: Antibacterial Efficacy of Compositions of the Invention
[0050] Prepare the antimicrobial composition for hard surface cleaning as shown in Table-1. The antimicrobial efficacy of the compositions was determined using the European Suspension Test BS EN1276 as briefly described below:
[0051] The test bacterial suspension (1 ml) was added to the samples of the product together with the specific soil (1 ml) at the specific dilution (8 ml). The mixture was kept at 20°C for 5 minutes. After this contact time, aliquots were collected and immediately equilibrated using proven methods. The number of bacteria in each sample was determined by plating on TSA agar, and the reduction in viable count was calculated after 48 hours of incubation.
[0052] In Table 1 below, the organism used was the bacterium Pseudomonas aeruginosa (P. aeruginosa) ATCC15442 (at a concentration of 10 7 cfu / ml); the dilution of the product (floor cleaner) in hard water is 1:70. Th...
Embodiment 4 to 6
[0057] Examples 4 to 6: Examples of short contact times
[0058] A similar test was carried out as in Examples 1 to 3, except that the dilution was 1:40 and the contact time between the product and the bacteria was 30 seconds instead of 5 minutes. The data are summarized in Table-2.
[0059] Table 2:
[0060] Element Example 4 (wt%) Example 5 (wt%) Embodiment 6 (wt%) Neodol (C12EO9) 3.0 3.0 3.0 Benzalkonium chloride 1.5 1.5 1.5 STPP 1.5 1.5 1.5 Sodium carbonate 0.3 0.3 0.3 Di / tricarboxylate - Sodium malonate 1.0 Sodium Citrate 1.0 Log reduction of bacteria 2.5 6.2 7.2
[0061] The data in Table 2 show that the antimicrobial compositions of the present invention (Examples 5 and 6) are effective even at short contact times of about 30 seconds.
Embodiment 7 to 15
[0062] Examples 7 to 15: Efficacy of compositions of the invention against various other bacteria
[0063] Compositions similar to Examples 1 to 3 were prepared and tested against various other types of bacteria as shown in Table-3. The contact time was 5 minutes and the product was diluted 1:200 with water. The soil used was 0.3% by weight bovine serum albumin (BSA).
[0064] The log reductions of the compositions and various bacteria are shown in Table-3.
[0065] table 3
[0066] Example product sample bacteria Log reduction 7 Example 1 Escherichia coli ATCC10536 2.5 8 Example 1 Staphylococcus aureus ATCC6538 3.5 9 Example 1 Enterococcus gilla ATCC10541 2.8 10 Example 2 Escherichia coli ATCC10536 5.8 11 Example 2 Staphylococcus aureus ATCC6538 6.5 12 Example 2 Enterococcus gilla ATCC10541 6.0 13 Example 3 Escherichia coli ATCC10536 7.2 14 Example 3 Staphylococcus aureus ATCC6538 7.2 ...
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