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Breeding Method of Regenerated Clones of Cinnamomum camphora Embryo Cultured Seedlings and Cut Section Plants

A technology for culturing seedlings and clones from embryos, which is applied in the field of plant regeneration camphora clonal breeding, can solve problems such as difficulty in obtaining transgenic plants of camphor, and achieve the effects of shortening the genetic transformation cycle of camphor, sufficient quantity and simplified steps.

Inactive Publication Date: 2016-08-17
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These two unfavorable factors ultimately make it difficult to obtain transgenic plants through genetically modified camphor

Method used

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  • Breeding Method of Regenerated Clones of Cinnamomum camphora Embryo Cultured Seedlings and Cut Section Plants
  • Breeding Method of Regenerated Clones of Cinnamomum camphora Embryo Cultured Seedlings and Cut Section Plants
  • Breeding Method of Regenerated Clones of Cinnamomum camphora Embryo Cultured Seedlings and Cut Section Plants

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Experimental program
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Embodiment 1

[0012] 1.1 Materials

[0013] At the end of July, the immature fruits on the robust, adult camphor trees were collected, the aseptically treated fruits were cut open, and the immature cotyledon embryos were taken out as research materials.

[0014] 1.2 Methods

[0015] 1.2.1 Disinfection of Cinnamomum camphora fruit and inoculation of cotyledon embryos

[0016] The treatment method is as follows: first soak the immature fruit of camphor (about 1cm in diameter) in the detergent solution for 30 minutes, then rinse it with running water for 30 minutes; then place it on the ultra-clean workbench, soak it in 75% alcohol for 30 seconds, 0.1 % mercuric chloride was soaked for 8 minutes, and finally rinsed with sterile water for 3 times; after sterilization of the camphor fruit, the fruit was cut, and the immature cotyledon embryos were taken out as research materials and inoculated on the cotyledon embryo germination induction medium.

[0017] 1.2.2 Culture methods and conditions ...

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Abstract

The invention discloses a clonal breeding method for regenerating high-frequency plants of a cinnamomumcamphoraL. embryo culture seedling stem segment. A cotyledon embryo in immature fruits of cinnamomumcamphoraL. is taken as a starting explant for building a cinnamomumcamphoraL. embryo culture seedling clone, and the embryo cultural seedling stem segment is taken as an adventitious bud induction explant, so as to build a high-frequency plant regeneration system. The clonal breeding method is characterized by comprising the following steps: building the explants of the cinnamomumcamphoraL. embryo culture seedling clones as immature cotyledon embryos of the cinnamomumcamphoraL.; selecting the cinnamomumcamphoraL. embryo culture seedling clone stem segments as the explants materials to build the plant regeneration system. The method disclosed by the invention is convenient to draw materials, wide in material source, sufficient in quantity, and easy to obtain sterile materials, and can play an important role in cinnamomumcamphoraL. tissue cultivation, genetic transformation and biotechnology improvement.

Description

technical field [0001] The invention belongs to the technical field of forestry, and in particular relates to a method for breeding a plant regeneration camphora clone. Background technique [0002] Camphor (Cinnamomum camphora L.), a plant of the Lauraceae family, is a representative tree species of subtropical evergreen broad-leaved forests. Its tree shape is beautiful, the branches are lush, the green shade covers the sun, and the momentum is majestic. It is an excellent common tree species for urban landscaping. The distribution in my country is generally bounded by the north of the Yangtze River, south to Guangdong and Guangxi and southwest, and mostly in southeastern coastal provinces such as Jiangxi, Zhejiang, and Fujian, so the cultivation range is limited by low temperature. In recent years, with the development of urban construction and new requirements of landscaping, in order to enrich the combination of tree species in northern gardens and improve the landscape...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杜丽姚瑶李勇鹏
Owner NANYANG NORMAL UNIV