Method for selecting adult state potential in-vitro flowering materials of cinnamomumcamphoraL. and regenerating plant

Abstract

The invention discloses a method for selecting adult state potential in-vitro flowering materials of cinnamomumcamphoraL. and regenerating a plant. The method concretely comprises the following steps: (1) selecting an annual branch with bulbel of adult health cinnamomumcamphoraL. of which the tree age is 8-10 years and the diameter at breast height is about 13.5cm as an explant source from the end of December to January of the next year; (2) inoculating the sterilized adult state stem segment with a node into a germination medium, and inducing stem segment germination; (3) inoculating germination branches into a propagation medium, carrying out multiplication to form individual clones, and building a stem segment cultured seedling propagation system; and (4) transferring the stem segments of stem segment culture seedlings into an induction medium to carry out adventitious bud induction culture, and building a culture seedling stem segment plant regeneration system of the cinnamomumcamphoraL. stem segment. The invention provides a cinnamomumcamphoraL. tissue culture method. According to the culture method, materials are conveniently obtained, well-sourced and sufficient in quantity, and a sterile material is easily obtained. The method for judging an adult state material is simple, and reliable in conclusion.

Description

technical field [0001] The invention relates to the technical field of forestry, in particular to a method for selecting and regenerating potential test-tube flowering materials of Cinnamomum camphora adult state. Background technique [0002] Camphor is a plant of the genus Cinnamomum lauraceae. This species has luxuriant branches and leaves, a large crown with thick shade, and a majestic tree. It is an excellent tree species for urban greening and is widely used as a shade tree, street tree, shelter forest and scenic forest. Because of its special aroma and volatile oil, it is moisture-resistant, anti-corrosion, insect-removing, and has a long shelf life. It is an ideal material for valuable furniture, high-level buildings, shipbuilding, and sculpture. Chemical raw materials: camphor and camphor oil, which are an important export material of our country; therefore, camphor, together with Nan, catalpa and paulownia, are called the four famous trees in the south of the Yangt...

AI Technical Summary

Problems solved by technology

The improvement of Cinnamomum camphora by biotechnology must be based on the establishment of an efficient plant regeneration system. However, at present, embryogenic callus of Cinnamomum camphora is mostly used as transgenic explant material, and the regeneration frequency of embryogenic callus is relatively high for Cinnamomum camphora. In addition, after the exogenous gene is transformed, it is necessary to add a certain concentration of antibiotics to the selection formula to screen the transformed materials, and the addition of antibiotics increases the difficulty of inducing its regeneration plants, which ultimately makes it difficult to obtain transgenic plants from camphor. The plants obtained from embryoid body regeneration have the same juvenile stage as seedlings (that is, woody plants can only enter the flowering and fruiting stage after going through a period of vegetative growth stage), so they are not suitable for flowering mechanism or transgenic inhibition of flowering. Research materials; the above factors restrict the process of improving camphor by biotechnology

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