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Compound microbial phosphate-solubilizing bacterial agent and preparation method thereof

A technology of composite microorganisms and phosphorus bacteria agents, which is applied in the field of agricultural microorganism utilization, can solve the problems that plants are difficult to directly absorb and utilize, and achieve the effects of reducing environmental pollution, promoting plant growth, and improving utilization

Inactive Publication Date: 2014-07-30
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Through research, it is found that phosphorus is one of the essential elements for plant growth, and 74% of the cultivated land in my country is deficient in phosphorus, and more than 95% of the phosphorus in the soil is in an invalid form, which is difficult for plants to directly absorb and utilize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Isolation and Screening of Efficient Phosphorus Solubilizing Bacteria

[0018] (1) Soil samples: collected from the rhizosphere of high-quality and high-yield wheat fields in northern Anhui Province, the surface soil of the wheat rhizosphere was removed, and the sampling depth was 5-20cm. Through the five-point sampling method, after sampling, screen, remove large mud and other solid impurities, mix well, seal with a clean fresh-keeping bag, and store in a 4°C refrigerator for later use.

[0019] (2) Organic phosphorus medium: NaCl 0.3g, magnesium sulfate heptahydrate 0.3g, manganese sulfate monohydrate 0.03g, KCl 0.3g, ammonium sulfate 0.5g, ferrous sulfate heptahydrate 0.03g, sucrose 10g, distilled water 1000mL, adjust pH 7.0-7.5, add 0.5g of calcium carbonate and 0.02g of soybean lecithin per 100mL of culture medium, if the above-mentioned medium is added with 15-20g of agar, it will be an organic phosphorus solid medium, and sterilize in a high-pressure ...

Embodiment 2

[0026] Example 2 Wheat Pot Experiment of Composite Microorganism Phosphorus Solubilizing Bacteria

[0027] Four treatments were set up in the wheat pot experiment, namely ① fertilization (chemical fertilizer) control, ② sterilized organic fertilizer + phosphate solubilizing bacteria agent, ③ sterilized organic fertilizer + sterilized phosphate solubilizing bacteria, ④ blank control, 6 repetitions, randomly arranged . The cultivation pot adopts a white plastic bucket with an upper diameter of 17 cm, a lower diameter of 12 cm, and a height of 13.5 cm.

[0028] Weigh 100g of seeds, wash them once with water, soak them in 75% alcohol for 3 minutes, wash them with sterile water for 6 times, and finally soak them with an appropriate amount of water, put them in an incubator at 25°C to accelerate germination, and sow the seeds when they turn white. Regular watering and management.

[0029] After the wheat was harvested, statistical analysis showed that the dry weight of wheat ker...

Embodiment 3

[0030] Example 3 Field Cultivation Experiment of Rapeseed with Composite Microorganism Phosphorus Solubilizer

[0031]Four treatments were set up in the experiment, namely ① fertilization (chemical fertilizer) control, ② sterilized organic fertilizer + phosphate solubilizing bacteria, ③ sterilized organic fertilizer + sterilized phosphate solubilizing bacteria, ④ blank control, repeated 3 times, and arranged randomly. The cultivation area is 2m×3m, and the blocks are randomly arranged. Regular watering and management.

[0032] After the rapeseed was harvested, statistical analysis showed that the dry weight of rapeseeds increased by 14.09% compared with the sterilized control, 9.88% higher than that of the fertilized (chemical fertilizer) control, and 23.65% higher than that of the blank control.

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Abstract

The invention discloses a compound microbial phosphate-solubilizing bacterial agent and a preparation method thereof. The effective ingredients of the compound microbial phosphate-solubilizing bacterial agent comprise pseudomonasveronii, rahnellaaquatilis and bacillusmegaterium. Three types of high-efficiency phosphate-solubilizing strains are obtained by screening soil samples in a high-yield wheat field in the north of Anhui Province, and the collection numbers of the pseudomonasveronii, the rahnellaaquatilis and the bacillusmegaterium are CGMCC No. 8741, CGMCC No. 8739 and CGMCC No. 8737 respectively. The preparation method comprises the following steps: blending and inoculating the pseudomonasveronii and the rahnellaaquatilis into an inorganic phosphorus liquid culture medium and culturing for 48 hours at the temperature of 28-30 DEG C and the speed of 180 r / min; inoculating the bacillusmegaterium into an organic phosphorus liquid culture medium and culturing for 96 hours at the temperature of 28-30 DEG C and the speed of 180 r / min; and uniformly blending a fermented bacteria agent and additives and packing after drying to obtain a finished product. The compound microbial phosphate-solubilizing bacterial agent is capable of remarkably increasing the yield of wheat, rape and other crops.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganism utilization, and relates to a compound microbial phosphate-dissolving agent and a preparation method thereof. Background technique [0002] my country is a large agricultural country. Fertilizers play a very important role in my country's grain production. The fertilizers currently on the market can be classified into two categories, one is chemical fertilizers, and the other is compound fertilizers that combine chemical fertilizers and organic fertilizers. Long-term use of chemical fertilizers will reduce soil fertility, reduce fertilizer utilization, destroy soil structure, and cause soil compaction. In addition to nitrogen, phosphorus, potassium and trace elements, compound fertilizer also contains organic matter. The central purpose of these fertilizers is to provide comprehensive chemical elements, but no consideration is given to improving the micro-organisms and micro-nu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C12R1/38C12R1/01C12R1/11
Inventor 邢素芝马忠友汪建飞赵建荣李孝良
Owner ANHUI SCI & TECH UNIV
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