Compound microbial phosphate-solubilizing bacterial agent and preparation method thereof
A technology of composite microorganisms and phosphorus bacteria agents, which is applied in the field of agricultural microorganism utilization, can solve the problems that plants are difficult to directly absorb and utilize, and achieve the effects of reducing environmental pollution, promoting plant growth, and improving utilization
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Embodiment 1
[0017] Example 1 Isolation and Screening of Efficient Phosphorus Solubilizing Bacteria
[0018] (1) Soil samples: collected from the rhizosphere of high-quality and high-yield wheat fields in northern Anhui Province, the surface soil of the wheat rhizosphere was removed, and the sampling depth was 5-20cm. Through the five-point sampling method, after sampling, screen, remove large mud and other solid impurities, mix well, seal with a clean fresh-keeping bag, and store in a 4°C refrigerator for later use.
[0019] (2) Organic phosphorus medium: NaCl 0.3g, magnesium sulfate heptahydrate 0.3g, manganese sulfate monohydrate 0.03g, KCl 0.3g, ammonium sulfate 0.5g, ferrous sulfate heptahydrate 0.03g, sucrose 10g, distilled water 1000mL, adjust pH 7.0-7.5, add 0.5g of calcium carbonate and 0.02g of soybean lecithin per 100mL of culture medium, if the above-mentioned medium is added with 15-20g of agar, it will be an organic phosphorus solid medium, and sterilize in a high-pressure ...
Embodiment 2
[0026] Example 2 Wheat Pot Experiment of Composite Microorganism Phosphorus Solubilizing Bacteria
[0027] Four treatments were set up in the wheat pot experiment, namely ① fertilization (chemical fertilizer) control, ② sterilized organic fertilizer + phosphate solubilizing bacteria agent, ③ sterilized organic fertilizer + sterilized phosphate solubilizing bacteria, ④ blank control, 6 repetitions, randomly arranged . The cultivation pot adopts a white plastic bucket with an upper diameter of 17 cm, a lower diameter of 12 cm, and a height of 13.5 cm.
[0028] Weigh 100g of seeds, wash them once with water, soak them in 75% alcohol for 3 minutes, wash them with sterile water for 6 times, and finally soak them with an appropriate amount of water, put them in an incubator at 25°C to accelerate germination, and sow the seeds when they turn white. Regular watering and management.
[0029] After the wheat was harvested, statistical analysis showed that the dry weight of wheat ker...
Embodiment 3
[0030] Example 3 Field Cultivation Experiment of Rapeseed with Composite Microorganism Phosphorus Solubilizer
[0031]Four treatments were set up in the experiment, namely ① fertilization (chemical fertilizer) control, ② sterilized organic fertilizer + phosphate solubilizing bacteria, ③ sterilized organic fertilizer + sterilized phosphate solubilizing bacteria, ④ blank control, repeated 3 times, and arranged randomly. The cultivation area is 2m×3m, and the blocks are randomly arranged. Regular watering and management.
[0032] After the rapeseed was harvested, statistical analysis showed that the dry weight of rapeseeds increased by 14.09% compared with the sterilized control, 9.88% higher than that of the fertilized (chemical fertilizer) control, and 23.65% higher than that of the blank control.
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