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Preparation method of an enzyme sensor and method for measuring hydrogen peroxide

A technology of enzyme sensor and hydrogen peroxide, which is applied in the field of enzyme sensor and electroanalytical chemical detection, can solve the problems of complicated and cumbersome hydrotalcite-modified electrode conductivity, etc., and achieve large specific surface area, good biocompatibility, and good conductivity sexual effect

Inactive Publication Date: 2017-01-25
EAST CHINA JIAOTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The object of the present invention is, aiming at the shortcomings of the complicated and cumbersome method for preparing horseradish peroxidase sensors with hydrotalcite as a carrier and the poor conductivity of hydrotalcite-modified electrodes, a simple one-step electrodeposition method can be used to modify carbon nanotubes based on carbon nanotubes. A horseradish peroxidase sensor supported by hydrotalcite was prepared on the electrode surface and used to detect hydrogen peroxide

Method used

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  • Preparation method of an enzyme sensor and method for measuring hydrogen peroxide
  • Preparation method of an enzyme sensor and method for measuring hydrogen peroxide
  • Preparation method of an enzyme sensor and method for measuring hydrogen peroxide

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Experimental program
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Effect test

Embodiment 1

[0021] The enzyme sensor was prepared with hydrotalcite as the carrier.

[0022] (1) Use a micro-syringe to take 3 μl of dispersed 0.5 mg / mL carbon nanotube suspension and evenly drop it on the surface of a clean glassy carbon electrode, let it dry naturally at room temperature, and rinse it with deionized water to obtain carbon nanotubes Modified electrodes.

[0023] (2) First prepare the electrodeposition solution containing 15 mmol / L cobalt nitrate, 5 mmol / L aluminum nitrate, 0.1 mol / L potassium nitrate and 0.3 mg / mL horseradish peroxidase, and then place the carbon nanotube modified electrode on the The constant potential deposition was carried out in the electrodeposition solution at -0.6 V for 80 s. After the deposition, it was rinsed with deionized water to obtain a hydrotalcite-horseradish peroxidase / carbon nanotube modified electrode. The oxidase-modified electrode is the enzyme sensor for the determination of hydrogen peroxide.

Embodiment 2

[0025] The enzyme sensor was prepared with hydrotalcite as the carrier.

[0026] (1) Use a micro-syringe to take 5 μl of dispersed 0.5 mg / mL carbon nanotube suspension and evenly drop it on the surface of a clean glassy carbon electrode, let it dry naturally at room temperature, and rinse it with deionized water to obtain carbon nanotubes Modified electrodes.

[0027] (2) First prepare the electrodeposition solution containing 21 mmol / L cobalt nitrate, 7 mmol / L aluminum nitrate, 0.2 mol / L potassium nitrate and 0.5 mg / mL horseradish peroxidase, and then place the carbon nanotube modified electrode on The constant potential deposition was carried out in the electrodeposition solution at -0.8 V for 70 s. After the deposition was completed, it was rinsed with deionized water to obtain a hydrotalcite-horseradish peroxidase / carbon nanotube modified electrode. The oxidase-modified electrode is the enzyme sensor for the determination of hydrogen peroxide.

Embodiment 3

[0029] The enzyme sensor was prepared with hydrotalcite as the carrier.

[0030] 1) Use a micro-syringe to take 5 μl of dispersed 1.0 mg / mL carbon nanotube suspension and evenly drop it on the surface of a clean glassy carbon electrode, let it dry naturally at room temperature, and rinse it with deionized water to obtain carbon nanotube modification electrode.

[0031] (2) First prepare the electrodeposition solution containing 22.5 mmol / L cobalt nitrate, 7.5 mmol / L aluminum nitrate, 0.3 mol / L potassium nitrate and 1.0 mg / mL horseradish peroxidase, and then place the carbon nanotube modified electrode on the The constant potential deposition was carried out in the electrodeposition solution at -0.9 V for 30 s. After the deposition was completed, it was rinsed with deionized water to obtain a hydrotalcite-horseradish peroxidase / carbon nanotube modified electrode. The oxidase-modified electrode is the enzyme sensor for the determination of hydrogen peroxide.

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Abstract

The invention relates to a preparation method for an enzyme sensor and a method for determining hydrogen peroxide. According to the method, a high catalytic performance of horse radish peroxidase to hydrogen peroxide is used and a signal enhancement effect of a carbon nano tube is utilized. According to the method, the enzyme sensor is prepared by loading hydrotalcite-enzyme on the surface of a carbon nano tube modified basic electrode through electrolytic deposition; a simple electrolytic deposition method is used for preparing hydrotalcite and fixing the horse radish peroxidase on the surface of an electrode so that the enzyme is tightly loaded on the surface of the electrode; the fixation of the enzyme and direct electron transmission of the enzyme are easy to realize.

Description

technical field [0001] The invention relates to the technical field of enzyme sensor and electroanalytical chemical detection, in particular to a preparation method and determination of an enzyme sensor for measuring hydrogen peroxide using hydrotalcite as a carrier. Background technique [0002] Hydrotalcite is a class of layered compounds composed of positively charged metal hydroxides and exchangeable anions filled between layers and layers. Due to its strong adsorption, large specific surface area, good biocompatibility and good ion exchange performance, hydrotalcite has been widely concerned in recent years and has been applied to enzyme sensors as a carrier and chemical modification of immobilized enzymes. electrode as a modifier. However, the weak conductivity of hydrotalcite is not conducive to the electron transfer of hydrotalcite-modified electrodes, which reduces the electrochemical performance of electrode materials and limits its vigorous development in the fie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/48
Inventor 杨绍明陈爱喜孙清李瑞琴尚培玲
Owner EAST CHINA JIAOTONG UNIVERSITY
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