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Preparation method of high-solubility low-phytic-acid soybean protein isolate

A technology of soybean protein isolate and high solubility, which is applied in the field of preparation of soybean protein isolate and low-phytic acid soybean protein isolate, can solve the problems of decreased separation effect, easy contamination of resin, decreased protein solubility and functional properties, etc., and achieves a simple production process. Ease of operation, industrial production, easy and fast enzymatic hydrolysis regulation effect

Active Publication Date: 2014-08-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the ion exchange method, because the resin is easy to bind to the protein, and the resin is easy to be contaminated, the application in actual production is limited
For ultrafiltration, due to the use of ultrafiltration membranes, protein macromolecules can easily block the pores of the membrane, which greatly reduces the separation effect, the yield of protein decreases, and the phytic acid content of the obtained protein is still relatively high.
The optimal pH of the phytase currently used in industry is an acidic environment, and the optimal temperature is between 55-65°C. Enzymolysis under this condition will have a serious impact on the production of soybean protein isolate, making the protein produce Aggregation and denaturation, long-term temperature treatment will also significantly reduce the solubility of proteins and other functional properties

Method used

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  • Preparation method of high-solubility low-phytic-acid soybean protein isolate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take the soybean meal to be processed, crush and sieve; the crushing condition is 35000rpm, time 30s, crush 3 times, each interval is 30s, the mesh number of the sieve is 100 mesh; take 100g soybean meal, press 1:10 (w / v) Mix the ratio with deionized water, stir evenly, adjust the pH to 7.0, stabilize the alkali solution under this condition for 1 hour, then centrifuge at 9500rpm for 30 minutes to obtain a protein solution, adjust the enzymolysis conditions and add 25 U / g soybean meal, time 30min, pH 5.0, temperature 40°C, then quickly adjust the pH to 4.5, carry out acid precipitation, centrifuge at 6000rpm, 30min to obtain curd, dissolve the curd and deionized water at a ratio of 1:10 (w / v) Disintegrate and neutralize to obtain SPI.

[0035] Further investigation and analysis of the phytic acid content and protein solubility of SPI showed that although the phytic acid content was reduced to 0.98mg / g, the protein solubility was also reduced to 80.65%.

Embodiment 2

[0037]Take the soybean meal to be processed, crush and sieve; the crushing condition is 30000rpm, time 1min, crush twice, each interval is 30s, the mesh number of the sieve is 80 mesh; take 100g of defatted soybean meal, press 1:10 (w / v ) and deionized water, stir evenly, adjust the pH to 7.0, and stabilize the alkali solution under this condition for 1 hour, then centrifuge at 9500 rpm for 30 minutes to obtain a protein solution, adjust the enzymolysis conditions and add 50 U / g soybean meal, Time 50min; pH 7.0, temperature 25°C, then quickly adjust the pH to 4.5, carry out acid precipitation, centrifuge at 6000rpm, 30min, to obtain curd, dissolve the curd and deionized water at a ratio of 1:10 (w / v) Afterwards, disintegrate and neutralize to obtain SPI.

[0038] Further investigation and analysis of the phytic acid content and protein solubility of SPI showed that the phytic acid content was reduced to 6.57mg / g, but compared with the control group, the content was still a bit...

Embodiment 3

[0040] Take the soybean meal to be processed, crush and sieve; the crushing condition is 30000rpm, time 1min, crush twice, each interval is 30s, the mesh number of the sieve is 80 mesh; take 100g of defatted soybean meal, press 1:10 (w / v ) with deionized water, stir evenly, adjust the pH to 7.0, and stabilize the alkali solution under this condition for 1 hour, then centrifuge at 9500 rpm for 30 minutes to obtain a protein solution, adjust the enzymolysis conditions and add 30 U / g soybean meal, Time 40min; pH 6.5, temperature 55°C, then quickly adjust the pH to 4.5, carry out acid precipitation, centrifuge at 6000rpm, 30min to obtain curd, dissolve the curd and deionized water at a ratio of 1:10 (w / v) Afterwards, disintegrate and neutralize to obtain SPI.

[0041] Further investigation and analysis of the phytic acid content and protein solubility of SPI showed that although the phytic acid content was reduced to 0.85mg / g, the protein solubility was also reduced to 67.56%.

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Abstract

A preparation method of a high-solubility low-phytic-acid soybean protein isolate belongs to the technical field of deep processing of soybeans. According to the preparation method, a soybean meal is firstly crushed to be good for the dissolution of the protein, then large particles like insoluble polysaccharide and fibers are screened to be removed, then a suspension liquid is prepared and then filtered and centrifuged after the protein is extracted through an alkaline process, the obtained protein solution is subjected to enzymolysis, proper optimization of the enzymolysis condition removes anti-nutritional factorphytic acid and keeps the solubleness of the protein, then acid precipitation and centrifugation are conducted, and the obtained milk curd is crushed, stirred, neutralized, sterilized and dried to obtain the soybean protein isolate. The preparation method is simple in operation and high in efficiency, the anti-nutritional factorphytic acid in the soybean protein isolate is effectively reduced, the protein is high in solubility under a neutral condition, and the preparation method is widely applied in the production like food processing, meat products, dairy processing.

Description

technical field [0001] The invention relates to a method for preparing soybean protein isolate with low phytic acid and high solubility, in particular to a method for obtaining soybean protein isolate with low phytic acid and high solubility by combining alkali-soluble acid precipitation and enzymatic treatment to degrade phytic acid. The invention belongs to the technical field of soybean deep processing. Background technique [0002] Soybean protein isolate is a plant-based protein material with a wide range of uses. It is a vegetable protein comparable to animal protein. The main method of domestic production of soybean protein isolate is alkali-soluble acid precipitation. The raw materials are mainly low-temperature defatted soybean meal after soybean oil extraction, soybean meal Contains higher phytic acid in soybean protein isolate, the content is as high as 20.89mg / g, and the phytic acid content in the prepared soybean protein isolate is also relatively high, the cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14
Inventor 华欲飞王红建孔祥珍陈业明张彩猛
Owner JIANGNAN UNIV
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