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Production method of efficient chelating enzyme peptide

A production method and chelation-type technology, which are applied in peptide preparation methods, microorganism-based methods, biochemical equipment and methods, etc., to maintain the health of intestinal flora, reduce poultry anemia, and reduce feeding costs.

Pending Publication Date: 2021-10-22
浙江康星生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems existing in the supplementation of existing poultry mineral elements, the present invention provides a method for producing mineral element high-efficiency chelating enzyme peptides by using bean dregs and soybean meal as raw materials through bacteria-enzyme collaborative fermentation and chelation

Method used

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  • Production method of efficient chelating enzyme peptide
  • Production method of efficient chelating enzyme peptide
  • Production method of efficient chelating enzyme peptide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Activation culture of Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, Lactobacillus rhamnosus and preparation of fermented seed liquid:

[0033] A, the preparation of culture medium:

[0034] Slope and plate medium: yeast extract 5g, peptone 10g, sodium chloride 10g, glucose 10g, agar 20g, distilled water 1000mL, sterilized at 121°C for 30min;

[0035]Seed liquid culture base: 20g soybeans, 20g brown sugar, 1000mL distilled water, make soybean milk, sterilize at 121°C for 30min;

[0036] B. Activation of various strains and preparation of fermented seed liquid:

[0037] Activation of Bacillus subtilis and preparation of fermented seed solution: Inoculate the Bacillus subtilis strains stored at 4°C on the slant medium, and culture at 37°C for 36h. Then streak a single colony on the plate, culture it upside down at 37°C for 36 hours, pick a single colony and inoculate it in a 250mL Erlenmeyer flask containing 50mL of seed liquid medium, and cul...

Embodiment 2

[0044] (1) The activation of Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, Lactobacillus rhamnosus and the preparation method of fermented seed liquid, concrete operation is the same as embodiment 1;

[0045] (2) Preparation of solid fermentation medium: the mass proportion of each component is: soybean dregs 60%, soybean meal 40%;

[0046] (3) The obtained Bacillus subtilis fermentation seed liquid is inoculated in the fermentation medium by the inoculum size of 8% of the quality of the solid fermentation medium, and the secondary powder of 24% of the quality of the solid fermentation medium is added, and water is added to make the water content 55% %, stir evenly, carry out the first stage of aerobic fermentation, the fermentation temperature is 36°C, and the aerobic fermentation is 24h;

[0047] (4) The fermented seed liquid of Saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus rhamnosus is inoculated respectively in the fermentation...

Embodiment 3

[0049] (1) The activation of Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, Lactobacillus rhamnosus and the preparation method of fermented seed liquid, concrete operation is the same as embodiment 1;

[0050] (2) Preparation of solid fermentation medium: the mass ratio of each component is: 40% bean dregs, 60% soybean meal;

[0051] (3) The obtained Bacillus subtilis fermented seed liquid is inoculated in the fermented medium by the inoculum amount of 10% of the quality of the solid fermented medium, and the secondary powder of 18% of the quality of the solid fermented medium is added, and water is added to make the water content be 40% %, stir evenly, carry out the first stage of aerobic fermentation, the fermentation temperature is 25°C, and the aerobic fermentation is 48h;

[0052] (4) The fermented seed liquid of Saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus rhamnosus is inoculated in the fermentation medium respectively accord...

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Abstract

The invention belongs to the field of biological feed, and relates to a production method of efficient chelating enzyme peptide. The method comprises the following steps: activating and propagating strains to respectively obtain bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus fermentation seed solutions; weighing bean dregs and bean pulp, and uniformly conducting stirring to prepare a solid fermentation culture medium; inoculating the bacillus subtilis fermentation seed solution, adding wheat middling, adjusting the water content, and carrying out first-stage aerobic fermentation; and inoculating the saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus fermentation seed solutions, protease and inorganic mineral salt, and carrying out second-stage anaerobic fermentation, so as to obtain the chelating enzyme peptide after the fermentation is finished. The mode of aerobic and anaerobic step-by-step fermentation, bacterium-enzyme synergistic fermentation and synchronous fermentation and chelation is adopted, the production process is simple, the chelating rate of various mineral elements in the obtained chelated enzyme peptide is high, chelated Fe mainly contains Fe<2+>, phytic acid of the low content, small peptide of the high content, organic acid and probiotics are contained, and economic benefits are remarkable.

Description

technical field [0001] The invention belongs to the field of biological feed, and in particular relates to a production method of high-efficiency chelating enzyme peptides of mineral elements suitable for poultry. Background technique [0002] Mineral elements such as Ca, Fe, Cu, Zn, Mn are involved in almost all life activities such as growth metabolism, signal transduction, immune regulation, and enzyme activation of laying hens and other poultry. Efficient production performance is of great significance. When the corresponding mineral elements are lacking in the feed, the poultry will grow slowly, the disease resistance will decline, the egg production rate will decrease, and the quality of the eggs produced will decrease. [0003] Poultry mineral supplementation has been developed through four generations. The first generation, inorganic salt type, such as: ferrous sulfate, copper sulfate, etc. Inorganic salt-type mineral elements have poor stability, low absorption u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12P21/06C12P39/00C07K1/107C12P7/40C12N1/20A23K50/75A23K20/147A23K20/20A23K20/24C12R1/865C12R1/25C12R1/225C12R1/125
CPCC12P21/00C12P21/06C12P39/00C07K1/107C12P7/40C12N1/20A23K50/75A23K20/147A23K20/30A23K20/24
Inventor 陈华友衡新宇陈华撑姚丹
Owner 浙江康星生物科技有限公司
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