Test strip for screening specific silk allergen immunoglobulin E (IgE), and preparation method of test strip

A technology of test strips and allergens, which is applied in the field of test strips and its preparation for specific silk allergen IgE screening, can solve the problems of cumbersome operation, poor sensitivity and precision, and long test time, and achieve operational Simple, accurate and low-cost effects

Active Publication Date: 2014-08-13
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are disadvantages of cumbersome operation, poor sensitivity and precision, and long test time

Method used

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Examples

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Embodiment Construction

[0027] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0028] Example The test strip used for the screening of specific silk allergen IgE and its preparation method

[0029] The preparation of test strips for specific silkworm allergen IgE screening includes the preparation of the antigen solution to be labeled, the preparation of the colloidal gold solution to be labeled, the colloidal gold labeled silkworm antigen, the purification of the colloidal gold silkworm antigen, the colloidal gold Size identification of protein particles and coating of test strips. Specific steps are as follows:

[0030] 1. Preparation of antigen solution to be labeled

[0031] The preparation method of silkworm ...

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Abstract

The invention provides a test strip for screening specific silk allergen immunoglobulin E (IgE). The test strip is enveloped by silk antigen labeled by colloidal gold. A colloidal gold-labeled technology is utilized, the specific silk allergen is labeled by the colloidal gold for the first time, and the labeled allergen is evenly sprayed onto a cellulose acetate membrane or a nitrocellulose membrane, so that the prepared test strip can be used for detecting the allergen. The test strip is easy and convenient to operate, accurate in results and low in cost, and is capable of realizing multiple kinds of free detection.

Description

technical field [0001] The invention relates to the technical field of colloidal gold labeling, in particular to a test strip for screening specific silk allergen IgE and a preparation method thereof. Background technique [0002] Allergic disease is the most common disease in contemporary society. It is caused by widespread allergens. About 5-10% of the population in my country and 5-25% of the population in the United States and Europe have suffered from allergic infestation. Among them, young children And teenagers are particularly obvious, and harm is more serious. Allergic reaction (allergy) is a pathological immune response that causes physiological dysfunction and tissue damage when the immune body is exposed to the same allergen again. Involves various clinical departments, such as internal medicine including bronchial asthma, anaphylactic shock, serum sickness, connective tissue disease, glomerulonephritis, migraine, etc., surgery including transplant rejection, oto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558
CPCG01N33/558G01N33/6893G01N2800/24
Inventor 蒋琳
Owner BIOSINO BIO TECH & SCI
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