Method for promoting sprouting of rosa multibracteata seeds

A multi-bud rose seed and multi-bud rose technology, applied in germination equipment, seed immunity, etc., can solve the problems of irregular germination and low germination rate of multi-bud rose seeds

Inactive Publication Date: 2014-08-20
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the present invention: In order to solve the problems of low germination rate and irregular germination of the multi-bud rose seeds afte

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] On October 3, 2007, 1 kg of mature rose fruit was collected in Jingzhou Village, Mao County, dripped with 10-15 ml of white wine (52°), covered with plastic film at 20-60°C, and then fermented for 5-10 days to rot the fruit skin. Then rinse the rose fruit with clean water.

[0016] 1) The rose seeds after rinsing are dried in the shade at a temperature of 10-25° C. and an air humidity of 25-65% for 5-10 days, and stored at room temperature for 7-15 days.

[0017] 2) Sterilize the water moss in an autoclave (108-121MP) for 30 minutes before use. Soak the seeds in warm water (30-45°C) for 24 hours, and mix them with the sterilized sphagnum moss. The mixing ratio is 4:1 (volume ratio) of sphagnum moss: seeds. At the same time, sterile water was added, and the sphagnum moss mixed with seeds was squeezed dry by hand until no more water could be squeezed out, then put into a ziplock bag, and placed in a 25°C incubator without light for 8 weeks. Open the ziplock bag every tw...

Embodiment 2

[0021] 1) On October 3, 2007, 1 kg of mature rose fruit was collected in Jingzhou Village, Mao County, dripped with 10-15 ml of white wine (52°), covered with plastic film at 20-60°C, and fermented for 5-10 days to make the peel rot, and then rinse the rose fruit with clean water;

[0022] 2) The rose seeds after rinsing are dried in the shade at a temperature of 10-25° C. and an air humidity of 25-65% for 5-10 days, and stored at room temperature for 7-15 days.

[0023] 3) Sterilize the water moss in an autoclave (108-121MP) for 30 minutes before use. Soak the seeds in warm water (30-45°C) for 24 hours, and mix them with the sterilized sphagnum moss. The mixing ratio is 4:1 (volume ratio) of sphagnum moss: seeds. At the same time, sterile water was added, and the sphagnum moss mixed with seeds was squeezed dry by hand until no more water could be squeezed out, then put into a ziplock bag, and placed in a 25°C incubator without light for 16 weeks. Open the ziplock bag every ...

Embodiment 3

[0027] 1) On October 3, 2007, 1 kg of mature rose fruit was collected in Jingzhou Village, Mao County, dripped with 10-15 ml of white wine (52°), covered with plastic film at 20-60°C, and fermented for 5-10 days to make the peel rot, and then rinse the rose fruit with clean water;

[0028] 2) The rose seeds after rinsing are dried in the shade at a temperature of 10-25° C. and an air humidity of 25-65% for 5-10 days, and stored at room temperature for 7-15 days.

[0029] 3) Sterilize the water moss in an autoclave (108-121MP) for 30 minutes before use. Soak the seeds in warm water (30-45°C) for 24 hours, and mix them with the sterilized sphagnum moss. The mixing ratio is 4:1 (volume ratio) of sphagnum moss: seeds. At the same time, sterile water was added, and the sphagnum moss mixed with seeds was squeezed dry by hand until no more water could be squeezed out, then put into a ziplock bag, and placed in a 25°C incubator without light for 8 weeks. Open the ziplock bag every t...

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Abstract

The invention discloses a method for promoting rosa multibracteata seeds to sprout. The method comprises the following steps including (1) rosa multibracteata fruit fermentation treatment; (2) seed drying in the air; (3) warm lamination: sphagna is sterilized in an autoclave sterilizer for 30 minutes, seeds are soaked for 24 hours in warm water and are mixed with the sterilized sphagna, meanwhile, sterile water is added, the sphagna mixed with the seeds is squeezed to a dry state by hand, the sphagna and the seeds are put into a reclosable bag, and the reclosable bag is placed into a 25 DEG C incubator for 8 to 16 weeks; (4) low-temperature lamination: the reclosable bag containing the sphagna and the seeds and subjected to warm lamination is placed into a 5 DEG C refrigerator, and the standing time is 4 to 16 weeks; and (5) seed sprouting. According to the method, measures of fruit fermentation, seed variable-temperature lamination and the like are adopted, the problems of low sprouting rate, long sprouting time and the like of the rosa multibracteata seeds are effectively solved, and the method has the advantages of simple process, low cost and the like. Through the implementation of the method, the sprouting rate is improved from 0 percent to 70 percents to 81.4 percents.

Description

technical field [0001] The invention belongs to the field of plant breeding, in particular to a method for promoting the germination of multi-bud rose seeds. Background technique [0002] Multi-bud rose (Rosa multibracteata Hemsl.&E.H.Wilson) is a shrub of the genus Rosa in the Rosaceae family. It is distributed in Sichuan, Yunnan and other provinces in my country, with an altitude distribution range of 1200-2600 meters. The height of the plant is 2.5 meters, the flowers are pink, 2-3 or several flowers form corymbs, the flowering period is May-July; the fruit is red, the fruit period is July-October. Rosa multibud is an important resource plant, and its plants contain active ingredients that can treat cardiovascular diseases (Chen Fengzheng, Zhao Weiquan, He Yonghua, Ding Lisheng, Wang Mingkui. Research on the chemical components of Sichuan and Yunnan rose and multibud rose. Application and environment Acta Biology, 2000, 6(4): 334-336); the fruit contains a variety of bene...

Claims

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Application Information

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IPC IPC(8): A01C1/02A01C1/08
Inventor 周志琼包维楷
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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