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Methods to decontaminate cereal grains with chlorine dioxide

A chlorine dioxide, grain technology, applied in the direction of biochemical equipment and methods, preservation of seeds with chemicals, application, etc.

Inactive Publication Date: 2014-08-20
JOHNSONDIVERSEY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such methods should not affect the quality of the final malt or products resulting from it

Method used

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  • Methods to decontaminate cereal grains with chlorine dioxide
  • Methods to decontaminate cereal grains with chlorine dioxide
  • Methods to decontaminate cereal grains with chlorine dioxide

Examples

Experimental program
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Effect test

example 1

[0059] Example 1: Treatment of Barley with Chlorine Dioxide (Artificial Contamination by Fusarium)

[0060] Investigation of the performance of chlorine dioxide in reducing initial Fusarium load on barley. Barley samples were artificially contaminated with Fusarium cells by spraying and drying before treatment with chlorine dioxide.

[0061] method. The chlorine dioxide solution was generated using Diversey's ClearKlens Bi-Spore Set (base VH26 and activator VH26). Prepare fresh stock solutions according to product instructions. Use Dr. Lange test kit (LCK310 / 343) and photometer DR2800 device to measure the concentration of chlorine dioxide in the stock solution and the use solution. With the help of CEN hard water diluent (300mg / kg CaCO 3 ) to prepare the chlorine dioxide use solution by diluting the Bi-Spore stock solution. The neutralizing solution was based on sodium thiosulfate (5 g / L in 1 / 4 strength Ringer's solution). The experiments were carried out on the basis o...

example 2

[0070] Example 2: Treatment of barley with chlorine dioxide added in a second steeping step in the steeping laboratory

[0071] More research was carried out in two trials involving a small steeping laboratory, in which harvested barley was treated with a chlorine dioxide solution in a second steeping. Again, a significant reduction in Fusarium counts was observed using chlorine dioxide for dipping.

[0072] Test program. Harvested barley from a high-load Fusarium batch was provided by the Czech Malt and Brew Institute. Initial probes (in parallel) were taken to determine the initial cell load on barley with Fusarium. Barley (1 g) was swirled in Ringer's solution (10 ml) to dissociate the cells. After vigorous vortexing, ten-fold dilution steps in sterile Ringer's solution were prepared. Pour over sterile potato agar medium from dilution step 10 -4 、10 -5 and 10 -6 Parallel 1ml samples. Chlorine dioxide-treated barley was preceded by a dilution series once washed with ...

example 3

[0082] Example 3: Treatment of barley with chlorine dioxide added in a second steeping step in a steeping laboratory. compared with blank control.

[0083] A comparative test of barley batches treated with chlorine dioxide in the second steeping against a control using only tap water was performed according to the procedure described in Example 2. Chlorine dioxide in the second impregnation solution was measured initially at 11 ppm and dropped to 8 ppm at the end of the impregnation (4 hours total, data not shown). The effect on Fusarium count reduction was determined. The results are presented in Table 5.

[0084] Barley was also analyzed for representative mycotoxin content as follows. High performance liquid chromatography with mass detection (LC-MS / MS) for the determination of selected Fusarium mycotoxins (deoxynivalenol, zearalenone) and deoxynivalenol in brewing raw materials -3-glucoside. After extraction, homogenized samples are filtered and purified on SPE column...

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Abstract

Methods are provided which include steeping and / or spraying a harvested cereal grain, such as barley, wheat, rye, millet, corn (maize), rice, or oats, in or with an aqueous solution including an effective amount of chlorine dioxide to provide a treated cereal grain. The treated cereal grain obtained from chlorine-dioxide steeping and / or spraying generally has a reduced microorganism and / or toxin content, and may be subjected to additional malting processes.

Description

[0001] Cross References to Related Patent Applications [0002] This patent application claims priority to US Provisional Patent Application No. 61 / 538,551 filed September 23, 2011, which is hereby incorporated by reference in its entirety. technical field [0003] The technology generally relates to the field of treating cereal grains with chlorine dioxide. Background technique [0004] The following instructions are provided to aid the reader's understanding. None of the information presented or references cited is admitted to be prior art on the present technology. [0005] Secondary growth of fungi in cereal grains is a common post-harvest problem that arises when (pre-infected) crops become infested with fungi during storage or food processing. A notable example of problems associated with secondary fungal growth is barley, which is commonly used in malting and brewing. Multiple Fusarium fungal species can contaminate harvested barley if the barley is grown during w...

Claims

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Application Information

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IPC IPC(8): A23B9/30A23L1/10A23L1/185A23L7/10A23L7/20A23L7/25
CPCA23L1/185A23L1/186A23B9/18C12C1/027A23B9/30C12C1/02A23L7/20A23L7/25
Inventor H.冯雷格P.巴萨K.科萨
Owner JOHNSONDIVERSEY INC
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