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Production method of extracting danshensu by ultramicronization combined with solid-state fermentation

A technology of solid-state fermentation and production method, which is applied in the production field of solvent extraction of Danshensu, can solve problems such as backward technology and difficult extraction and utilization of active ingredients, and achieve the effects of advanced technology, improved extraction utilization rate, and improved extraction rate

Inactive Publication Date: 2016-02-10
HUBEI UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the fat-soluble components and water-soluble components are separated from Salvia miltiorrhiza, but in the production of processing and extracting its active ingredients, due to the backward technology, it is difficult to extract and utilize all the active ingredients by simple crushing and extraction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Taking the production of 2.5g Danshensu as an example

[0019] Take 100g of Salvia miltiorrhiza powder, 50g of bran, 15g of Bacillus subtilis seed liquid, and 900ml of 70% ethanol.

[0020] The preparation method is as follows:

[0021] 1) Salvia miltiorrhiza is selected to remove roots, washed to remove impurities, sliced ​​and dried to a moisture content of 5%. Pulverize to 20 mesh with a powder machine, and repeatedly pulverize to a particle size of 15 μm with a jet mill.

[0022] 2) Weigh 100 grams of microprocessed salvia miltiorrhiza powder, fully mix with 50 grams of bran, and sterilize at 121° C. for 20 minutes in a high-pressure steam sterilizer.

[0023] 3) Inoculate 15g of Bacillus subtilis seed solution, add sterile water to a moisture content of 40%, put it in a constant temperature incubator at 35°C, and ferment for 4 days.

[0024] 4) Take out the fermented base material, add 900ml of 70% ethanol, reflux extraction for 2 hours, continue t...

Embodiment 2

[0025] Embodiment 2: Taking the production of 5g Danshensu as an example

[0026] Take 200g of Salvia miltiorrhiza powder, 100g of bran, 30g of Bacillus subtilis seed liquid, and 1800ml of 80% ethanol.

[0027] The preparation method is as follows:

[0028] 1) Salvia miltiorrhiza is selected to remove roots, washed to remove impurities, sliced ​​and dried to a moisture content of 5%. Pulverize to 20 mesh with a powder machine, and then repeatedly pulverize to a particle size of 15 μm with a jet mill.

[0029] 2) Weigh 200 grams of the microprocessed salvia miltiorrhiza, fully mix with 100 grams of bran, and sterilize at 121° C. for 20 minutes in a high-pressure steam sterilizer.

[0030] 3) Inoculate 30g of Bacillus subtilis seed solution, add sterile water to a moisture content of 40%, put it in a constant temperature incubator at 35°C, and ferment for 4 days.

[0031] 4) Take out the fermented base material, add 1800ml of 80% ethanol, reflux extraction for 1.5 hours, cont...

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Abstract

The invention provides a producing method for extracting tanshinol through combining ultra-refining with solid-state fermentation. According to the method, the weight ratio of salvia miltiorrhiza fine powder to wheat bran is 2:1, and the inoculation quantity of bacillus subtilis liquid is 10 percent of the total weight of the salvia miltiorrhiza fine powder and the wheat bran. A preparing method comprises the following steps that salvia miltiorrhiza subjected to impurity removal and cleaning are crushed into fine powder with the granularity being smaller than or equal to 15[mu]m; after the fine powder is mixed with the wheat bran, the bacillus subtilis is inoculated, sealed fermentation is carried out for 4 days, and ethanol reflux is adopted for extracting the tanshinol. Finished products produced by the producing method provided by the invention have the obvious efficacies of removing stasis to relieve pain, promoting blood circulation to remove meridian obstruction, clearing away the heart-fire to relieve restlessness and nourishing the body.

Description

technical field [0001] The invention relates to a processing method of danshensu, in particular to a production method of crushing danshensu cells by ultramicronization combined with solid-state fermentation, and solvent extracting danshensu. Background technique [0002] Salvia miltiorrhiza is a perennial herb. It has the functions of dispelling blood stasis, relieving pain, promoting blood circulation, clearing heart, eliminating troubles and nourishing in Chinese medicine. Modern pharmacological studies have shown that salvia miltiorrhiza has significant effects on the cardiovascular system and blood system. Danshen mainly contains fat-soluble and water-soluble components. At present, fat-soluble components and water-soluble components are isolated from Salvia miltiorrhiza, but in the production of processing and extracting its active components, due to backward technology, it is difficult to extract and utilize all the active components, especially by simple crushing and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C59/52C07C51/42
CPCC07C51/42C07C59/52
Inventor 汤尚文刘相久于博豁银强吴进菊郭壮夏小梅
Owner HUBEI UNIV OF ARTS & SCI
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