Disposable supporting cell culture system for sterile cell culture bag

Abstract

The invention provides a disposable supporting cell culture system for a sterile cell culture bag. The disposable supporting cell culture system comprises a stirring unit, a bracket pipeline, a temperature control unit, a gas path control circuit and a controller unit for controlling the four units, wherein the bracket pipeline is respectively connected with the stirring unit, the temperature control unit and the gas path control unit, and used for providing a channel for gas and liquid needed for the system; the stirring unit is provided with a stirring paddle which is integrated with a hollow gas distributor; the hollow gas distributor is connected with the gas path control unit; the hollow gas distributor comprises a micro air bubble distributor arranged on the lower part of the stirring paddle and a ventilating pipe arranged inside the stirring paddle. The system is used for lowering the labor intensity, staff needed for production and GMP (good manufacturing practice) production workshop area are greatly lower than those of the conventional production equipment, thereby facilitating realizing quicker refit for conveniently operating novel flow.

Description

technical field [0001] The invention relates to a cell culture system, in particular to a cell culture system matched with a disposable aseptic cell culture bag. Background technique [0002] Large-scale culture technology (large-scale culture technology) refers to the technology of cultivating cells at a high density in a cell bioreactor (bioreactor) under artificial conditions (setting pH, temperature, dissolved oxygen, etc.) for the production of biological products. [0003] At present, large-scale cell culture mainly adopts spinner bottle culture system and stainless steel tank culture system. [0004] Spinner bottle culture has the following problems: limited surface area, low cell growth density; unstable monitoring and control of environmental conditions (pH, dissolved oxygen, etc.) during culture, affecting product quality; high labor intensity, large space occupation, and high pollution rate. [0005] Stainless steel tank culture has the following problems: the ne...

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Problems solved by technology

[0004] Spinner bottle culture has the following problems: limited surface area, low cell growth density; unstable monitoring and control of environmental conditions (pH, dissolved oxygen, etc.) during culture, affecting product quality; high labor intensity, large space occupation, and high pollution rate

[0005] Stainless steel tank cultivation has the following problems: the need for complex cleaning-in-place (CIP) or in-place sterilization (SIP) pipelines, the possibility of cross-contamination between process runs; long preparation time for each production batch, labor-intensive production personnel Large; the shear force of the stirring system is large, and the cells are seriously damaged

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