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A method for extracting α-glucosidase inhibitors from Phylonotus pine

A technology of glucosidase and P. pine fungus, which is applied in the direction of medical raw materials derived from fungi, drug combinations, metabolic diseases, etc., and can solve problems such as gastrointestinal reactions and drug resistance, high economic pressure, and high prices , to achieve the effects that are conducive to development, have low toxic and side effects, and high safety

Active Publication Date: 2017-01-18
浙江慧和健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the above-mentioned α-glucosidase inhibitory drugs used for the treatment of type 2 diabetes have a certain effect on controlling blood sugar, they need to be taken for a long time, and there are many adverse reactions, such as hypoglycemia, lactic acidosis, gastrointestinal reactions and drug resistance. etc., have certain limitations in the use of
Moreover, the α-glucosidase inhibitors used in China are mainly imported or patented drugs, which are relatively expensive, and the economic pressure on most diabetic patients who take drugs for a long time is relatively high.

Method used

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  • A method for extracting α-glucosidase inhibitors from Phylonotus pine
  • A method for extracting α-glucosidase inhibitors from Phylonotus pine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Fruiting body of Phylopore pine → shredded to 0.4cm 3 Left and right small pieces → Weigh 50g → CO 2Supercritical degreasing (supercritical extraction apparatus: Applied Separation, USA, SFE-Prime), pressure 20MPa, temperature 40°C, static extraction for 1h, dynamic discharge for 0.5h→ raffinate was dried with hot air at 50°C for 2h and crushed→add the weight of raw materials 20 times of 80% ethanol aqueous solution → microwave boiling extraction for 20 minutes (microwave extractor: Shanghai Xinyi Microwave Chemical Technology Co., Ltd., MAS-Ⅱ, power 600W) → centrifuge to remove slag → supernatant concentrated in vacuum (45°C, 0.05MPa) To dry → add 100mL of distilled water, the purpose of adding distilled water here is to wash and transfer the above-mentioned rotary evaporation dried product (rotary evaporated dried product will stick to the wall, and it is impossible to dry thoroughly, and secondary drying is required), and the second is to Pre-freeze for refrigerator...

Embodiment 2

[0035] The fruiting bodies of Phymopore pine → shredded to 0.3cm 3 Left and right small pieces → Weigh 50g → CO 2 Supercritical degreasing (supercritical extraction apparatus: Applied Separation, USA, SFE-Prime), pressure 30MPa, temperature 45°C, static extraction for 1.5h, dynamic discharge for 0.5h → raffinate was dried with hot air at 45°C for 3h and crushed → adding raw materials 30 times the weight of 95% ethanol aqueous solution → microwave boiling extraction for 40 minutes (microwave extractor: Shanghai Xinyi Microwave Chemical Technology Co., Ltd., MAS-Ⅱ, power 600W) → centrifugation to remove slag → vacuum concentration of the supernatant (40°C, 0.05MPa ) to dryness→add 80mL distilled water→vacuum dry at 40°C and 0.06MPa→Inhibitor of Phylonotus pinewood α-glucosidase (containing 18.5% of total phenols)→IC of semi-inhibitory activity of α-glucosidase 50 =0.057 mg / mL.

Embodiment 3

[0037] Fruiting body of Phylopore pine → shredded to 0.4cm 3 Left and right small pieces → Weigh 50g → CO 2 Supercritical degreasing (supercritical extraction apparatus: Applied Separation, USA, SFE-Prime), pressure 20MPa, temperature 40°C, static extraction for 1h, dynamic discharge for 0.5h→ raffinate was dried with hot air at 50°C for 2h and crushed→add the weight of raw materials 20 times 70% methanol aqueous solution → microwave boiling extraction for 20 minutes (microwave extractor: Shanghai Xinyi Microwave Chemical Technology Co., Ltd., MAS-Ⅱ, power 600W) → centrifuge to remove slag → supernatant concentrated in vacuum (45°C, 0.05MPa) To dryness→add 90mL distilled water→freeze→Fominus pinewood α-glucosidase inhibitor (containing 13% of total phenols)→α-glucosidase semi-inhibitory activity IC 50 =0.081 mg / mL.

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Abstract

The invention provides a method of extracting an alpha-glucosidase inhibitor from phellinus pini. The method includes: cutting phellinus pini sporocarps into pieces, performing CO2 supercritical extraction for degreasing by a supercritical extractor, drying the extract with hot wind having a temperature of 40-50 DEG C for 2-3 h, smashing, adding an aqueous methanol or ethanol solution having a concentration of 70-95% (v / v) and having a weight of 15-30 times of the weight of the extract, performing microwave boiling extraction for 20-40 min, filtering or centrifuging to remove residue, subjecting the liquid supernatant to vacuum concentration, and freezing or drying in vacuum to obtain the alpha-glucosidase inhibitor. The beneficial effects of the method are that: the history of phellinus pini being adopted as medicines in civil is long, the phellinus pini is low in toxicity and side effects and high in safety, the method is free of toxic and harmful solvent and high in safety, the prepared alpha-glucosidase inhibitor is high in activity, and the method is beneficial for development of subsequent medicines and healthcare foods.

Description

[0001] (1) Technical field [0002] The invention relates to a method for extracting α-glucosidase inhibitor from Phylonotus pine. [0003] (2) Background technology [0004] Medicinal fungi are a class of higher fungi with high medicinal value. They generally have several or more functions such as anti-tumor, hypoglycemic, hypolipidemic, anti-viral, and immune-enhancing. [0005] Diabetes is a chronic and multiple endocrine disorder disease, which is regarded as the world's three major diseases together with cancer and cardiovascular disease. Long-term hyperglycemia in diabetic patients can cause damage to microvessels, large vessels, and nerves, resulting in dysfunction of the eyes, kidneys, cardiovascular system, and nervous system, and various complications. In recent decades, the incidence of diabetes has been rising rapidly due to factors such as human overnutrition, excessive work pressure, and population aging. According to statistics from the world-renowned medical j...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/07A61P3/10
Inventor 杨开邢辰金月忠孙培龙
Owner 浙江慧和健康科技有限公司