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Method for detecting fingerprints of toad bufanolide diene compositions in toad medicinal materials and application of method

A technology of bufadiene and fingerprints, which is applied in the field of analysis and detection of traditional Chinese medicine components, can solve the problems of reduced service life of chromatographic columns, tedious and time-consuming problems, and achieve the effect of fast, accurate, good stability and high sensitivity of the detection method

Active Publication Date: 2014-09-10
HEHUANG PHARMA SHANGHAI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the complex buffer salt system will reduce the service life of the chromatographic column and the tedious and time-consuming solution preparation

Method used

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  • Method for detecting fingerprints of toad bufanolide diene compositions in toad medicinal materials and application of method
  • Method for detecting fingerprints of toad bufanolide diene compositions in toad medicinal materials and application of method
  • Method for detecting fingerprints of toad bufanolide diene compositions in toad medicinal materials and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Determination of fingerprints of bufadiene components in toad venom

[0061] 1 Experimental part

[0062] 1.1 Sample pretreatment

[0063] Preparation of the test solution: Accurately weigh 0.2g of toad venom fine powder, accurately add 20ml of methanol, weigh, ultrasonically extract, after cooling, weigh again, make up for weight loss with methanol, shake well, filter, and take the filtrate As the test solution.

[0064] Preparation of reference substance solution: Accurately weigh an appropriate amount of reference substances of bufafetalin, bufafetalin, bufafolin, cinobufafolin base, and lipobufagenin reference substance respectively, add methanol to dissolve and constant volume, and shake well to obtain Mix the reference substance solution; wherein, the content range of each component is: bufafetalin 151.2-226.8 μg, bufafetalin 225.6-451.2 μg, bufafolin 171.6-343.2 μg, cinobufacyl 460.0-9200.0 μg, Repobugenin 394.4 ~ 788.8μg.

[0065] 1.2 Chromatograph...

Embodiment 2

[0118] Example 2 Determination of content of 5 kinds of bufadiene components (bufafalin, bufafolin, bufalin, cinobufafogin, and lipobufagenin) in the medicinal material of toads

[0119] 1 Experimental part

[0120] 1.1 Sample pretreatment

[0121] Preparation of the test solution: Accurately weigh 0.2g of toad venom fine powder, accurately add 20ml of methanol, weigh, ultrasonically extract, after cooling, weigh again, make up for weight loss with methanol, shake well, filter, and take the filtrate As the test solution.

[0122] Preparation of reference substance solution: Accurately weigh an appropriate amount of reference substances of bufafetalin, bufafetalin, bufafolin, cinobufafolin base, and lipobufagenin reference substance respectively, add methanol to dissolve and constant volume, and shake well to obtain Mix the reference substance solution; wherein, the content range of each component is: bufafetalin 151.2 μg, bufafetalin 225.6 μg, bufafolin 171.6 μg, cinobufafol...

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Abstract

The invention provides a method for detecting fingerprints of toad bufanolide diene compositions in toad medicinal materials. The method comprises the main steps: preparing a to-be tested sample solution and a reference sample solution, determining and obtaining the liquid chromatograms of the to-be tested sample solution and the reference sample solution, and comparing the liquid chromatogram of the to-be tested sample solution with the liquid chromatogram of the reference sample solution, so as to obtain the fingerprints of five toad bufanolide diene compositions. The invention further provides application of the above fingerprint detection method to determine the content of the five toad bufanolide diene compositions in the toad medicinal materials. By analyzing the fingerprints and the content of the toad bufanolide diene compositions, the inner quality of toad medicinal materials can be reflected, basis is provided for determining true and false, evaluating advantages and disadvantages and investigating stability and consistency of toad medicinal materials, and reference is provided for clinical administration of toads. Also, the established detection method is rapid, accurate and high in sensitivity, and can be used as a reliable means for qualitative quantitative control on quality and application of toads.

Description

technical field [0001] The invention relates to the technical field of analysis and detection of traditional Chinese medicine components, in particular to a method for detecting fingerprints of bufadiene components in toad venom and its application. Background technique [0002] The 2010 edition of "Chinese Pharmacopoeia (Part One)" records that Toad Su is a medicinal material obtained by processing and drying the white serum secreted by the post-auricular glands and skin glands of Bufo bufo gargarizansCantor or Bufo melanostictus Schneider. Its properties are oblate lumps or flakes. The lumps are hard and not easy to break. The cross-section is brown, horny and slightly shiny; the flakes are brittle and fragile, and the cross-section is reddish brown and translucent. . The taste of toad's supper is sweet at first, and then has a long-lasting spicy feeling. The powder smells like a sneeze. It has the effects of detoxification, pain relief, resuscitation and refreshment. It...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 顾佳敏姜鹏陈忠樑詹常森向阳朱佳娴许雯雯
Owner HEHUANG PHARMA SHANGHAI
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