Application of soybean transcription factor GmMYB172 in vegetable oil and fat metabolism regulation
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of plant and oil content, applied in the biological field, can solve problems such as little known
Inactive Publication Date: 2014-09-24
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
View PDF1 Cites 5 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, in plants, the regulatory mechanism of lipid sy
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0044] Example 1, cDNA cloning of the transcription factor GmMYB172 encoding gene related to the regulation of soybean and lipid metabolism and the construction of the plant expression vector
[0045] 1. Acquisition of transcription factor GmMYB172
[0046]A gene of the MYB transcription factor family, GmMYB172, was highly expressed during the analysis of fatty acid accumulation metabolism during soybean seed development.
[0047] Soybean Suinong 14 extracted (Hefeng 25 / Suinong 8, a new mid-maturing spring soybean variety bred by Suihua Agricultural Science Institute of Heilongjiang Academy of Agricultural Sciences, see China Agricultural Science and Technology Network, http: / / shucai.ag365.com / postdetail_351344.html. Provided by Manweiqun, Heilongjiang Academy of Agricultural Sciences. Recorded in the following documents: Qin Jun et al., Genetics, 28 (11), 1421-1427, 2006. Obtained) total RNA of seedlings, and the RNA was reverse-transcribed with reverse transcriptase to syn...
Embodiment 2
[0056] Embodiment 2, the acquisition of transgenic GmMYB172 Arabidopsis
[0057] 1. Obtaining recombinant Agrobacterium
[0058] The recombinant vector pPROKII-GmMYB172 obtained in Example 1 was introduced into Agrobacterium GV3101 by electroporation (Lee CW et al., Agrobacterium tumefaciens promotes tumor induction by modulating pathogen defense in Arabidopsis thaliana, Plant Cell, 2009, 21 (9), 2948-62; public available Obtained from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences), pick the recombinant Agrobacterium, extract the plasmid of the recombinant Agrobacterium and verify it by BamHI and KpnI double digestion, the insert fragment is a positive plasmid of about 222bp, and the recombination containing the positive plasmid Agrobacterium was named GV3101 / pPROKII-GmMYB172.
[0059] 2. Acquisition and identification of transgenic GmMYB172 Arabidopsis
[0060] The recombinant Agrobacterium GV3101 / pPROKII-GmMYB172 was cultivated to the log...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses oil and fat metabolism regulation related soybean transcription factor GmMYB172 and a coding gene and application thereof. The protein is named as GmMYB172, is derived from papilionaceae glycine soybean (Glycine max (L.) Merrill), is a protein as follows (a) or (b): (a) protein is composed of an amino acid sequence shown as the sequence 2 in the sequence table; and (b) protein is a protein obtained by substitution and/or deletion and/or addition of one or a plurality of amino acid residues of the amino acid sequence shown as the sequence 2 and is in relevant to plant tissue total oil and fat content and derived from the sequence 2. Experiments show that, the transcription factor GmMYB172 and the coding gene can regulate the oil and fat content in plant seeds, and after over expression, can improve the oil and fat content in the plant seeds. The coding gene has important theoretical and practical significances in the aspects of increasing and improving crop oil and fat ingredients, especially improving oil and fat ingredients in soybean and other oil plant seeds, and cultivating high oil and fat containing varieties.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to the application of soybean transcription factor GmMYB172 in the regulation and control of vegetable oil metabolism. Background technique [0002] 71% of the fat in the human diet comes from plants. Among several major oil-producing crops in the world, the total oil production of soybean accounts for about 30%, ranking first in the world's vegetable oil production (Table 1). [0003] Table 1 shows the main oil-producing crops in the world [0004] [0005] The synthesis of fatty acids is one of the most important metabolic pathways in plants. It exists in any cell of plants and is necessary for growth and development. Blocking it leads to cell death, so a plant mutant that blocks fatty acid synthesis has not been found so far. [0006] Plants differ greatly from other eukaryotes in the enzymes involved in the fatty acid synthesis pathway. Synthesis of fatty acids with 16 or 18 ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more