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Bio-safety evaluation method of nano zinc oxide based on Caco-2 cells

A nano-zinc oxide, biosafety technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as poor correlation and predictability

Active Publication Date: 2014-09-24
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems are as follows: 1. The dose of poisoning is the main issue in the risk and hazard assessment of nanomaterials
Despite the often poor correlation and predictability between in vitro models and in vivo observations, there are currently no scientific criteria for moving from material synthesis to animal models

Method used

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  • Bio-safety evaluation method of nano zinc oxide based on Caco-2 cells
  • Bio-safety evaluation method of nano zinc oxide based on Caco-2 cells
  • Bio-safety evaluation method of nano zinc oxide based on Caco-2 cells

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Experimental program
Comparison scheme
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Embodiment Construction

[0028] Material

[0029] cell line

[0030] Human Colon Adenocarcinoma Epithelial Caco-2 Cells Shanghai Institute of Cell Biology, Chinese Academy of Sciences

[0031] main reagent

[0032] Nano Zinc Oxide Particles Hangzhou Wanjingxin Co., Ltd.

[0033] L-Glutamine Hyclone Dispense

[0034] Penicillin U.S. Sigma distribution

[0035] Streptomycin American Sigma distribution

[0036] Mitomycin C in Roche, Switzerland

[0037] DMEM American Hyclone Corporation

[0038] Dimethyl sulfoxide (DMSO) U.S. Hyclone Fetal Bovine Serum Hangzhou Sijiqing Biotechnology Co., Ltd.

[0039] 10xPBS Biyuntian Biotechnology Co., Ltd.

[0040] MTT Kit Biyuntian Biotechnology Co., Ltd.

[0041] 0.25% pancreatin Biyuntian Biotechnology Co., Ltd. 1.5ml, 15ml, 50ml centrifuge tube Corning, USA

[0042] 25cm and 75cm cell flasks Corning, USA

[0043] 6-well plate, 24-well plate and 96-well plate Corning, USA

[0044] main instrument

[0045] JEM-1011 Transmission Electron Microscope Japan...

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Abstract

The invention provides a bio-safety evaluation method of nano zinc oxide based on Caco-2 cells. The method comprises the following steps: conventionally culturing cells, passaging the cells, freezing the cells, thawing the cells, contaminating the cells, performing morphological observation and detecting the cell activity. The method has certain theoretical meaning on discussion on intestinal toxicity of nano zinc oxide; a reference is provided for establishment of food nano zinc oxide limit standards, a reliable scientific basis is provided for further study on the safety problem of food nano zinc oxide, a reference is provided for safety evaluation of other nanomaterials, and theoretical support is provided for safety use of nano zinc oxide.

Description

technical field [0001] The invention belongs to the field of food safety detection, in particular to a biological safety evaluation method for nano-zinc oxide based on Caco-2 cells. Background technique [0002] From the current research progress, it can be known that the generation of reactive oxygen species and the change of oxidative stress in the body is the well-known toxicity mechanism of nanomaterials. From the characteristics of nanomaterials: the smaller the particle size, the larger the specific surface area, the more active sites for the reaction, and the easier it is to generate free radicals with high reaction ability. In addition, different nanomaterials have different physical and chemical properties and different metabolic activities in the tested organisms, so the toxic mechanisms of different nanomaterials are also different. In addition to the mechanism of oxidative stress, factors such as the release of zinc ions, inflammatory responses and surface elect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 关荣发康天舒王彦波伍义行吕飞张进杰
Owner CHINA JILIANG UNIV
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