Determination method of content of schisandrin in medicament

A technique for determination of schisandrin methyl alcohol is applied in the field of determination of the content of pharmaceutical components, which can solve the problems of long time, short detection time, difficult separation, etc., and achieve the effects of reducing usage, shortening detection time, and reducing analysis cost.

Inactive Publication Date: 2014-09-24
SHIJIAZHUANG YILING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with traditional HPLC, UPLC has the advantages of short detection time and high resolution. It can overcome the shortcomings of long time and difficult separation when used for the detection of Schisandrin A in Hugan Tablets.

Method used

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  • Determination method of content of schisandrin in medicament
  • Determination method of content of schisandrin in medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for assaying content of Schisandrin A in medicine, comprising the following steps:

[0041] A. Preparation of test sample solution: Weigh 2.5g of the drug, grind it finely, place it in a Soxhlet extractor, add n-hexane to the extractor and soak it for 12 hours, reflux extraction at 80°C for 6 hours, and the extract is evaporated at low temperature. Dry, then add methanol slightly heat to dissolve, shake well, that is;

[0042] B. Preparation of reference substance solution: Weigh an appropriate amount of schisandra alcohol A reference substance, add methanol to make solutions containing 0.02mg, 0.05mg, 0.1mg, 0.2mg, and 0.5mg per 1ml, to obtain final product;

[0043] C. UPLC detection: the chromatographic column is C18, the mobile phase is methanol-water, the ratio of methanol-water is 72:28, and the flow rate is 0.30ml min -1 , the injection volume is 2μl, and the detection wavelength is 254nm;

[0044] D, the formulation and calculation result of standard ...

Embodiment 2

[0046] A method for assaying content of Schisandrin A in medicine, comprising the following steps:

[0047] A. Preparation of test sample solution: Weigh 5g of the drug, grind it finely, add n-hexane to soak for 24 hours, reflux and extract at 85°C for 6 hours, evaporate the extract to dryness at low temperature, then add methanol to slightly heat to dissolve, shake well , that is;

[0048] B. Preparation of reference substance solution: Weigh an appropriate amount of schisandra alcohol A reference substance, add methanol to make a solution containing 0.02mg, 0.05mg, 0.1mg, 0.2mg, 0.5mg per 1ml, to obtain final product;

[0049] C. UPLC detection: the chromatographic column is C18, the mobile phase is methanol-water, the ratio of methanol-water is 72:28, and the flow rate is 0.30ml min -1 , the injection volume is 2μl, and the detection wavelength is 254nm;

[0050] D, the formulation and calculation result of standard curve: the reference substance solution of each concentr...

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Abstract

The invention discloses a determination method of the content of schisandrin in medicaments, which particularly comprises the following steps of: preparation of a sample solution to be tested, preparation of a control solution, UPLC detection, establishment of a standard curve, and result calculation. The method of the invention is short in detection time and high in separation degree.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method for determining the content of drug components. Background technique [0002] Hugan Tablet has the functions of soothing the liver and regulating qi, invigorating the spleen and eliminating food, and reducing transaminase. For chronic hepatitis and early cirrhosis. In the 10th edition of the Pharmacopoeia, HPLC is used for the test of Hugan Tablets, and Schizandrin B takes about 40-60 minutes by HPLC, and it is difficult to separate. Compared with traditional HPLC, UPLC has the advantages of short detection time and high resolution. It can overcome the shortcomings of long time and difficult separation when used for the detection of Schisandrin A in Hugan Tablets. Contents of the invention [0003] The technical problem to be solved in the present invention is to provide a method for determining the content of schisandrol A in medicines with short detection time and high...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 霍际伟于国江李净祁星星柴振平
Owner SHIJIAZHUANG YILING PHARMA
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