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The function and application of nucleotide synthase cad gene in the treatment of fatty liver and type Ⅱ diabetes

A technology of nucleotide synthase and fatty liver, applied in the field of invention, can solve problems such as liver damage, aggravate heart burden, increase blood volume, etc., and achieve the effect of improving fatty liver

Active Publication Date: 2016-06-08
武汉惠康基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008]4) Insulin sensitizers work by activating PPAR-, and their biggest side effects are liver damage and increased blood volume, thus increasing the burden on the heart

Method used

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  • The function and application of nucleotide synthase cad gene in the treatment of fatty liver and type Ⅱ diabetes
  • The function and application of nucleotide synthase cad gene in the treatment of fatty liver and type Ⅱ diabetes
  • The function and application of nucleotide synthase cad gene in the treatment of fatty liver and type Ⅱ diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] [Example 1] Mouse fatty liver and type Ⅱ diabetes model (DIO) obtained

[0051] 1. Grouping of experimental animals: 8-week-old, male, WT mice and CAD-KO mice were selected and fed with two special feeds, D12942 high-fat diet (Highfatdiet, HFD) and D12450B low-fat diet (Normalchow, NC). That is WTNC group, KONC group, WTHFD group, KOHFD group a total of 4 groups.

[0052] 2. Operation process of high-fat feed induction model:

[0053] Using WT and KO mice, the DIO model was established, and the phenotype correlation analysis was carried out to clarify that the CAD gene plays an important role in the pathogenesis of metabolic diseases. 8-week-old, male, WT mice and CAD-KO mice were selected and fed with two special diets, D12942 high-fat diet (Highfatdiet, HFD) and D12450B low-fat diet (Normalchow, NC), respectively, namely WTNC group and KONC group , WTHFD group, KOHFD group a total of 4 groups. The food intake of the mice was recorded in detail every week, and the f...

Embodiment 2

[0054] [Example 2] mouse body weight, blood glucose level determination

[0055] (1) Detection of fasting body weight and food intake of mice, and blood collection from orbital sinus

[0056] 1) Weight detection

[0057] ①Fasting: Fast the mice to be tested at 8:00 am (without water), and start the experimental operation at 2:00 pm.

[0058] ② Weighing: Weigh at 0 week, 4 week, 8 week, 12 week, 16 week, 20 week and 24 week respectively, put a small plastic bucket on the dynamic electronic balance, grab the mouse, put it into the weighing In a small bucket, measure the weight and record the data. Feed amount detection: After the weighing operation is completed, add feed to the mice, and record the amount of feed for the mice on the dynamic electronic balance.

[0059] (2) Detection of fasting blood glucose level

[0060] All the mice to be tested were fasted from 8:00 am to 2:00 pm (without water), that is, the experimental operation was started after 6 hours of fasting.

...

Embodiment 3

[0066] [Example 3] Glucose tolerance test (intraperitonealglucosettolerancetest, IPGTT)

[0067] On the 22nd week of the experiment, the intraperitoneal glucose injection test (IPGTT) was performed to evaluate the glucose tolerance of the mice.

[0068] ① Before measuring blood glucose, measure the fasting body weight of the mice, and calculate the injection volume of glucose based on 10 μL / g.

[0069] ② First test the fasting blood glucose at 0 minutes before the glucose injection, and inject the glucose solution through the abdominal cavity quickly after the test is completed.

[0070] ③Intraperitoneal injection operation method: ①Fix the mouse; grab the mouse, grasp the tail of the mouse with the little finger and ring finger of the left hand, and grab the neck of the mouse with the other three fingers, so that the head of the mouse is down, and the mouse The abdomen is fully exposed. ②Needle positioning and injection: insert the needle from the side of the abdomen, hold ...

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Abstract

The invention discloses the function and application of a nucleotide synthase CAD gene in fatty liver and type II diabetes, and belongs to the field of gene function and application. The present invention studies the function of the CAD gene through a model induced by a high-fat diet. As a result, it is found that the body weight and fasting blood glucose level of the CAD gene knockout mice in the HFD group are higher than those of the WT mice in the control group. The glucose tolerance of the mice was significantly weakened; the general appearance of the liver, the weight of the liver, the ratio of liver to body weight, and the pathological staining results of lipid components all showed that the fatty liver lesions of the CAD knockout mice in the HFD group were obviously serious, and the lipid Accumulation was significantly increased, and these results indicated that CAD gene knockout significantly worsened fatty liver and type 2 diabetes. Aiming at the above-mentioned effects of CAD, CAD can be used to prepare medicines for preventing, alleviating and / or treating fatty liver and / or type II diabetes.

Description

technical field [0001] The invention belongs to the field of gene function and application, and particularly relates to a nucleotide synthetase CAD: carbamoyl-phosphate synthetase II, aspartate aminotransferase and dihydroorotase (carbamoyl-phosphatesynthetase II, aspartatetranscarbamylase, anddihydroorotase (CAD) in fatty liver and type Ⅱ diabetes mellitus, specifically in the application of drugs for the prevention, alleviation and / or treatment of fatty liver and / or type Ⅱ diabetes. Background technique [0002] Diabetes is a chronic metabolic disease that seriously endangers human health. It is estimated that the number of patients will increase to 439 million by 2030, which means that 7.7% of adults aged 20-79 in the world will be diabetics. In the past two or three decades, the number of people with diabetes has doubled, and it is an extremely serious threat to human health around the world. Currently, the incidence of type 2 diabetes and pre-type 2 diabetes among chil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K38/43A61P1/16A61P3/10
Inventor 李红良王丕晓张晓东杜成
Owner 武汉惠康基因科技有限公司
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