Method for testing seven indicative PCBs in food with high fat content simultaneously

A technology for polychlorinated biphenyls and a determination method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome pretreatment operation steps, high requirements for pretreatment equipment, long detection time, etc., and achieves low requirements for pretreatment equipment. , Solve the effect of high requirements for pre-processing equipment and fast detection speed

Inactive Publication Date: 2014-10-01
FUQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing methods, there is no good solution for the determination of the seven indicative PCBs in high-fat samples, and there are high require...

Method used

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  • Method for testing seven indicative PCBs in food with high fat content simultaneously
  • Method for testing seven indicative PCBs in food with high fat content simultaneously
  • Method for testing seven indicative PCBs in food with high fat content simultaneously

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take olive oil as a sample:

[0029] Weigh 2.00 g of olive oil sample into a 50 mL stoppered centrifuge tube, add 2.0 mL of n-hexane to dissolve the sample, add 25.0 mL of acetonitrile, vortex for 1 min, add 10.0 g of anhydrous sodium sulfate, vortex for 30 s, The centrifuge tube was centrifuged at 4500 r / min for 3 min, and all the supernatant was poured into a 100 mL chicken heart bottle; 25.0 mL of acetonitrile was added to re-extract once, and the supernatant was combined and rotary evaporated to near dryness at 40 °C; with 20.0 mL Transfer the sample extract from the above 100 mL chicken heart bottle to a 50 mL stoppered centrifuge tube in three times (10 mL, 5 mL, 5 mL) with n-hexane, add 2.00 mL concentrated sulfuric acid, vortex for 1 min, and rotate at 4500 r / min Centrifuge at a rotating speed for 3 min, add 2.0 mL of deionized water evenly, vortex for 15 s, and centrifuge at a speed of 4500 r / min for 3 min, absorb the sulfuric acid solution in the lower l...

Embodiment 2

[0045] Take Pomfret as a sample:

[0046] Weigh 5.00 g of pomfret sample into a 50 mL stoppered centrifuge tube, add 2.0 g of sodium chloride and 10.0 g of anhydrous sodium sulfate, add 25.0 mL of acetonitrile, disperse and homogenize at 11000 rpm for 1 min, and centrifuge The tube was centrifuged at 4500 r / min for 3 min, and all the supernatant was poured into a 100 mL heart-shaped bottle; re-extracted once with 25.0 mL of acetonitrile, and the supernatant was combined and rotary evaporated to near dryness at 40 °C; with 20.0 mL of Transfer the sample extract in the above 100 mL chicken heart bottle into a 50 mL stoppered centrifuge tube with n-hexane three times (10 mL, 5 mL, 5 mL), add 2.00 mL of concentrated sulfuric acid, vortex for 1 min, and rotate at 4500 r / min Centrifuge for 3 minutes, add 2.0 mL of deionized water evenly, vortex for 15 s, and centrifuge at a speed of 4500 r / min for 3 minutes, use a straw to absorb the sulfuric acid solution in the lower layer, ...

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Abstract

The invention relates to a method for testing seven indicative PCBs (PCB28, PCB52, PCB101, PCB118, PCB138, PCB153 and PCB180) in food with high fat content simultaneously. The method comprises the following steps: using an organic solvent to conduct extraction, concentration and decreaming on a target object and conduct two times of purification on an extracting solution, forming a standard curve, analyzing the test condition, and conducting methodology investigation and practical sample testing on factors such as linearity, recovery rate, precision and detection limit of the seven indicative PCBs in different samples. The method does not need to equip expensive pretreatment equipment to remove fat in the sample and use column chromatography pretreatment process which is complex, low in efficiency and low in flux, has the advantages of being quick in testing speed, wide in adaptive substrate range, high in sensitivity, strong in selectivity, high in accuracy and the like, can meet detection requirements on the seven indicative PCBs in the sample with high fat content by a conventional laboratory, moreover can test the content of the seven indicative PCBs in the sample with high fat content at the same time, and has the characteristics of being simple, convenient, fast, accurate and the like.

Description

technical field [0001] The invention belongs to the technical field of food safety detection and relates to a method for simultaneously detecting seven kinds of indicative polychlorinated biphenyls in foods with high fat content. Background technique [0002] Polychlorinated biphenyls have "teratogenic, mutagenic, and carcinogenic" hazards to the human body, and their chemical properties are stable and difficult to be degraded. They can migrate through various carriers and accumulate in the human body through the food chain. The Global Environmental Monitoring System / Food Planning Section (GEMS / Food) stipulates PCB28, PCB52, PCB101, PCB118, PCB138, PCB153 and PCB180, a total of 7 polychlorinated biphenyl homologue isomers as indicative monomers of PCBs pollution status (Indicator PCBs), instead of all polychlorinated biphenyl congener isomers for detection. Since polychlorinated biphenyls have become an important factor that endangers human health, countries have set corres...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 丁立平
Owner FUQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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