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Application of Vinexin-beta in treating cerebral apoplexy

A stroke and disease technology, applied in gene therapy, nervous system diseases, cardiovascular system diseases, etc.

Active Publication Date: 2014-10-08
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far there is no literature report on the application of Vinexin-β in stroke disease

Method used

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  • Application of Vinexin-beta in treating cerebral apoplexy
  • Application of Vinexin-beta in treating cerebral apoplexy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Acquisition of mouse cerebral infarction model (I / R)

[0029] 1. Grouping of experimental animals: wild-type mice and Vinexin-β gene knockout mice, cerebral infarction model (I / R) was established by middle cerebral artery ischemia-reperfusion. They were randomly divided into two groups, 10 mice in each group: wild-type mice I / R group (WT I / R), Vinexin-β gene knockout mouse I / R group (KO I / R).

[0030] 2. The I / R operation of cerebral infarction by suture method adopts the MCAO (middle cerebral artery occlusion, middle cerebral artery occlusion) model operation process:

[0031](1) Grab the mouse, use 3% isoflurane to anesthetize the mouse, remove the mouse hair on the neck with 8% sodium sulfide, quickly cut off the mouse hair on the top of the skull with surgical scissors, and disinfect the neck and skull with 3% iodine. Top skin 2 times, 75% alcohol deiodination 1 time.

[0032] (2) Make a transverse incision on the top of the skull of the mouse to expose ...

Embodiment 2

[0036] Example 2 Determination of Cerebral Infarction Volume in Cerebral Infarction Model (I / R) Mice

[0037] The evaluation indicators of the severity of cerebral ischemia / reperfusion injury mainly include cerebral infarct volume and neurological function score, and these indicators are positively correlated with the severity of ischemia / reperfusion injury.

[0038] (1) Neurological function and behavioral scores were performed 24 hours and 72 hours after surgery before sampling;

[0039] Improved method based on Berderson neurological function score (9-point scale):

[0040] 0 points: no symptoms of nerve damage;

[0041] 1 point: The contralateral forelimb is curled up when the tail is raised, or the forelimb on the affected side cannot be fully reached;

[0042] 2 points: The opposite shoulder is adducted when the tail is raised;

[0043] 3 points: flat push: the resistance decreases when pushing to the opposite side;

[0044] 4 points: can move spontaneously in all di...

Embodiment 3

[0057] Example 3 Determination of Apoptosis of Neurons in the Peripheral Area of ​​Brain Tissue Infarction

[0058] 1. Brain Tissue Cryosection Preparation

[0059] (1) Grab the mouse and anesthetize the mouse by intraperitoneally injecting 3% pentobarbital sodium.

[0060] (2) Open the chest to expose the heart, puncture the left ventricle with an injection needle, and cut the right atrium at the same time.

[0061] (3) Perfuse with PBS (0.01M, pH 7.4) at a pressure of 100 mmHg until the liver turns white, and then perfuse with 4% paraformaldehyde for 15 minutes.

[0062] (4) The brain of the mouse was taken out quickly after craniotomy, and fixed with 4% paraformaldehyde at room temperature for 6-8 hours.

[0063] (5) The olfactory bulb and cerebellum of the brain tissue were removed, and then the brain was divided into two parts along the midline, and then fixed with the previous fixative for 15 minutes.

[0064] (6) Submerge in PBS (0.01M, pH 7.4) containing 30% sucrose...

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Abstract

The invention discloses application of Vinexin-beta in treating cerebral apoplexy, belonging to the field of gene functions and application. By using the Vinexin-beta gene knockout mouse as the experimental subject, the arteria cerebri media ischemia-reperfusion injury model research on the relationship between Vinexin-beta gene and cerebral apoplexy proves that the brain infarction volume of the Vinexin-beta gene knockout mouse is obviously reduced as compared with the wild type control mouse, the neural function is obviously improved, and the brain dead neuron quantity is obviously reduced, thereby detecting that the Vinexin-beta gene can promote the actions of the deteriorating neural function and cerebral apoplexy. Therefore, the Vinexin-beta can be used as a pharmaceutical target for screening drugs for protecting the neural functions and preventing, relieving and / or treating cerebral apoplexy; and the Vinexin-beta inhibitor can be used for preparing drugs for protecting the neural functions and preventing, relieving and / or treating cerebral apoplexy.

Description

[0001] technical field [0002] The invention belongs to the field of gene function and application, and particularly relates to the application of Vinexin-β in the treatment of cerebral apoplexy, especially in the preparation of drugs for preventing, alleviating and / or treating cerebral apoplexy. Background technique [0003] Stroke is the fourth leading cause of death in the world, second only to cardiovascular diseases, tumors and chronic lower respiratory diseases, and it is also one of the most important causes of lifelong disability. Among them, about 80% of the patients are ischemic strokes, the main cause of which is insufficient blood supply and oxygen supply to brain tissue caused by cerebral vascular obstruction or cerebral hemorrhage. It is predicted that the cost of stroke treatment in the United States from 2005 to 2050 will be about 152 billion U.S. dollars based on the purchasing power in 2005, which has caused a huge burden on the economy and health. In Chi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68A61K45/00A61K48/00A61K39/395A61P25/00A61P9/10
CPCA61K45/00C12Q1/6883C12Q2600/136
Inventor 李红良郭森卢燕云郑安康李明昌
Owner WUHAN UNIV
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