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Hairtail fingerprint spectrum construction method

A technology of fingerprint spectrum and construction method, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of no fast and effective detection and difficulty in determining the origin of hairtail, and achieve good reliability, high accuracy and precise detection results. high degree of effect

Active Publication Date: 2014-10-15
ZHEJIANG MARINE DEV RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The object of the present invention is to provide a method based on high-efficiency liquid that is simple to operate, high in detection efficiency, reliable in detection results, fast in speed, and high in accuracy in order to solve the defects that the origin of hairtail is difficult to determine and there is no method for quickly and effectively detecting the origin of hairtail. Construction Method of Hairtail Fingerprint by Phase Chromatography

Method used

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  • Hairtail fingerprint spectrum construction method
  • Hairtail fingerprint spectrum construction method
  • Hairtail fingerprint spectrum construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042]A method for constructing hairtail fingerprints, said method comprising:

[0043] a) Preparation of the test solution: choose hairtail samples that have a shiny body surface, bright red gills, transparent mucus, full eyeballs, and clear corneas. Take 1.5g hairtail muscle samples and dry them at 0 to -10°C for 12-18 hours , for subsequent use; then add 5 times of hairtail quality deionized water and stir to obtain hairtail pulp, use 0.36% papain of hairtail quality and 0.2% chitosan of fish pulp quality, stir for 240min at 35°C, and the pH is 5.5; after stirring, adjust the pH to 10, keep the pH for 30min, adjust the pH to neutral, then centrifuge at 4000rpm, take the supernatant, dry the supernatant completely to obtain the hairtail sample solid, add the hairtail sample solid to acetic acid Ethyl ester was refluxed and ultrasonically extracted for 40 minutes, centrifuged, and the supernatant was added to the basic alumina chromatography column, the column liquid was coll...

Embodiment 2

[0050] A method for constructing hairtail fingerprints, said method comprising:

[0051] a) Preparation of test solution: hairtail samples are selected from hairtails with shiny body surface, bright red gills, transparent mucus, full eyeballs, and clear corneas. Take 5g hairtail muscle samples and dry them at 0 to -10°C for 12-18 hours. Standby; add the hairtail sample that has passed through a 55-mesh sieve to methanol for reflux and ultrasonic extraction for 40 minutes, centrifuge, take the supernatant and add it to the basic alumina chromatography column, collect the column liquid, then elute with water, and pass through the column solution and water eluent, evaporated to dryness, dissolved in methanol and fixed to 20mL, and then passed through a 0.35μm microporous membrane as the test solution; wherein, the gradient elution method was adopted, and the elution procedure is shown in Table 1;

[0052] b) High-performance liquid chromatography measurement: absorb the test solu...

Embodiment 3

[0055] A method for constructing hairtail fingerprints, said method comprising:

[0056] a) Preparation of the test solution: choose hairtail samples with shiny body surface, bright red gills, transparent mucus, full eyeballs, and clear corneas. Take 4.5g hairtail muscle samples and dry them at 0 to -10°C for 12-18 hours , for subsequent use; then add deionized water of 6 times the quality of hairtail and stir to obtain hairtail pulp, use 0.97% papain of hairtail quality and 0.33% chitosan of fish pulp quality, stir for 30min at 40°C, and the pH is 7. After stirring, adjust the pH to 11.2, keep the pH for 45 minutes, adjust the pH to neutral, then centrifuge at 4000rpm, take the supernatant, dry the supernatant completely to obtain the hairtail sample solid, add the hairtail sample solid to acetic acid Ethyl ester was refluxed and ultrasonically extracted for 40 minutes, centrifuged, and the supernatant was added to the basic alumina chromatography column, the column liquid wa...

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Abstract

The invention relates to a hairtail fingerprint spectrum construction method which is as follows: haumela is prepared into a to-be-tested product solution for studious testing by high performance liquid chromatograph (HPLC) to obtain a hairtail fingerprint spectrum; the method comprises to-be-tested product solution preparation, high performance liquid chromatograph detecting, and standard fingerprint spectrum determination. According to the method, the hairtail fingerprint spectrum is established by HPLC chromatography, the methodological investigation shows that the method is reliable and good in repeatability, and meets the fingerprint spectrum requirements; the hairtail producing area detection method has the advantages of simple operation, high detection efficiency, fast and reliable detection results, high precision, and high accuracy rate of more than 92.7%.

Description

technical field [0001] The invention relates to a method for constructing hairtail fingerprints based on high-performance liquid chromatography with simple operation, high detection efficiency, good reliability of detection results, high speed and high precision. [0002] Background technique [0003] Hairtail ( Trichiurus lepturus ), also known as tooth belt, white belt fish, saury, etc., belongs to the fish class Perciformes hairtail family animals, it is a migratory fish in the middle and lower layers of the warm temperature, inhabits the muddy seabed at a water depth of 60-100m, and is a variety of fish, hairy shrimp They mainly eat squid and squid, and have obvious diurnal vertical movement. They are widely distributed in warm and tropical seas around the world (mostly in the Indian Ocean and the Western Pacific). [0004] The hairtail fish body is obviously flattened on the side, extending into a band shape, and the tail is slender like a whip. The general body lengt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 周宇芳郑斌肖金星相兴伟杨会成孟志娟徐珊
Owner ZHEJIANG MARINE DEV RES INST
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