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Preparation method of hydroxysafflor yellow A

A technology of hydroxysafflower and yellow pigment, which is applied in the field of medicine and can solve the problems of cumbersome, complicated and high-cost preparation processes

Active Publication Date: 2014-10-22
ZHEJIANG YONGNING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the similar structure of safflower yellow compounds and their strong water solubility, the preparation process of pure hydroxysafflower yellow A is cumbersome and costly. Domestic research has not achieved satisfactory results, and the widespread extraction process Long time-consuming, high energy consumption, low yield and other problems
The Chinese patent (publication number: CN101195647A) adopts macroporous adsorption resin to carry out initial purification to the crude product of hydroxysafflower yellow A, and then uses gel resin to further purify, and the sample purity obtained can reach more than 99%. The dextran gel resin is expensive, and its yield is only 50%, the processing cycle is long and complicated, the application is difficult, and it is not easy to apply in large-scale industrial production
However, high-speed countercurrent chromatography instruments are expensive and can only prepare a small amount of samples each time, which cannot meet the large-volume demand for drug development

Method used

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  • Preparation method of hydroxysafflor yellow A
  • Preparation method of hydroxysafflor yellow A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 100g of safflower medicinal material (hydroxysafflor yellow A content of 1.7%), add 1L of 10% ethanol, ultrasonically extract twice at 40℃, 1h each time, filter the extract, combine the filtrate, concentrate to 1L, use 2M sodium hydroxide Adjust the solution to pH=9, and apply the macroporous adsorption resin column to the ratio of the above sample solution to the resin weight ratio of 1:1 (macroporous adsorption resin column specification and model: 1000mL, microsphere No. 1, Shanghai Huazhen Technology Co., Ltd.) , The height to diameter ratio of the macroporous adsorption resin column is 10:1, and the sample flow rate is 2bV / h. After the sample is loaded and adsorbed, deionized water is used as the eluent, and the elution flow rate is 2bV / h. The effluent is collected, and the effluent is detected by HPLC. 1.51 g of 99.3% hydroxysafflor yellow A powder (yield 88.8%).

[0030] HPLC detection method: C18 column, Agilent HP1260 liquid chromatograph, mobile phase metha...

Embodiment 2

[0032] The column height to diameter ratio of the macroporous adsorption resin column for purification was adjusted to 8:1, and the rest was the same as in Example 1, and finally obtained 99.3% hydroxysafflor yellow A1.48g. (Yield 87.1%)

Embodiment 3

[0034] The column height-to-diameter ratio of the macroporous adsorption resin column for purification was adjusted to 5:1, and the rest was the same as in Example 1. Finally, 1.40 g of 99.2% hydroxysafflor yellow A was obtained. (Yield 82.4%)

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Abstract

The invention belongs to the field of medicines, relates to a purification preparation method of a traditional Chinese medicine red flower medicinal component, and specifically relates to a preparation method of hydroxysafflor yellow A. A purifying method of the hydroxysafflor yellow A adopts macroporous adsorption resin for purifying, and adopts water or low-concentration alcohol for eluting, so that the purity of the finally obtained hydroxysafflor yellow A exceeds 99%, and yield of the hydroxysafflor yellow A exceeds 80%. The preparation method disclosed by the invention is simple to operate, low in cost, high in product purity, high in yield and suitable for large-scale industrial production preparation of the hydroxysafflor yellow A.

Description

Technical field [0001] The invention belongs to the field of medicine, and relates to a purification preparation technology of medicinal components of Chinese medicine safflower, in particular to a preparation method of hydroxysafflor yellow A. Background technique [0002] The traditional Chinese medicine safflower is derived from the dried flowers of the Compositae plant Carthamus tinctorious L., it is pungent and warm in nature, and has the effect of promoting blood circulation, clearing blood stasis and relieving pain. Modern pharmacological studies have shown that the main active components of safflower are concentrated in water-soluble safflower yellow pigment. Safflor yellow is a flavonoid compound, which has the effects of dilating coronary arteries, anti-oxidation and anti-inflammatory. It is also a platelet activating factor inhibitor, which can inhibit the formation of thrombus and is used for the treatment of cardiovascular and cerebrovascular diseases. Further resea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H7/04C07H1/06
Inventor 叶天健阚士东李文颖马苏旺
Owner ZHEJIANG YONGNING PHARMA