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Method for detecting content of silver ions through fluorescence

A technology of ion content and silver ions, applied in the field of analysis and detection, can solve the problems of silver ion fluorescence quenching, lack of universality, low sensitivity, etc., and achieve the effects of easy popularization and application, good sensitivity and simple operation

Active Publication Date: 2014-10-22
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, due to the characteristics of simple operation and high sensitivity, fluorescence spectroscopy has been widely used in the detection of various ions, such as Zn 2+ , Ca 2+ 、Cu 2+ However, due to the fluorescence quenching effect of silver ions, there are relatively few methods for using fluorescent probes to detect silver ions
Most of the current fluorescence detection methods are for specific samples, lack of universality and low sensitivity

Method used

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  • Method for detecting content of silver ions through fluorescence
  • Method for detecting content of silver ions through fluorescence
  • Method for detecting content of silver ions through fluorescence

Examples

Experimental program
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Effect test

Embodiment 1

[0045] The DNA molecule capable of forming an i-motif structure used in this example is a cytosine-rich single-stranded DNA molecule with a sequence of 5'-CCACCACCACCACAACCACCACCCACCAAA-3' (its nucleotide sequence is shown in sequence 1); The cyanine dye that uses is the compound shown in formula II:

[0046]

[0047] (1) The process of i-motif / cyanine dye fluorescence detection of silver ion concentration is as follows:

[0048] 1) Preparation of DNA mother solution: Dissolve the DNA molecule shown in Sequence 1 in 10 mM PBS buffer solution (disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution) containing 1 mM EDTA (ethylenediaminetetraacetic acid) at a pH of 7.4 , Recipe: EDTA was dissolved in 10 mM PBS buffer solution with a pH value of 7.4 to make the final concentration 1 mM) to prepare a DNA stock solution with a DNA concentration of 100 μmol / L for future use.

[0049] 2) Preparation of cyanine dye mother liquor: Weigh a certain amount of cyanine d...

Embodiment 2

[0058] The DNA molecule capable of forming an i-motif structure used in this example is a cytosine-rich single-stranded DNA molecule with a sequence of 5'-CCTTCCCCACCCCTCCCCACCCTCCCCA-3' (its nucleotide sequence is shown in sequence 2); The cyanine dye that uses is the compound shown in formula III:

[0059]

[0060] (1) The process of i-motif / cyanine dye fluorescence detection of silver ion concentration is as follows:

[0061] 1) Preparation of DNA mother solution: the DNA molecule shown in Sequence 1 was dissolved in 10 mM PBS buffer solution containing 1 mM EDTA (ethylenediaminetetraacetic acid) (complexed with other metal ions to prevent interference with test results) with a pH value of 7.4 ( Disodium hydrogen acid-sodium dihydrogen phosphate buffer solution) (recipe: dissolve EDTA in PBS buffer solution with 10 mM and pH value of 7.4, so that the final concentration is 1 mM), and prepare DNA with a DNA concentration of 100 μmol / L Mother liquor, spare.

[0062] 2) P...

Embodiment 3

[0071] The DNA molecule capable of forming an i-motif structure used in this example is a cytosine-rich single-stranded DNA molecule with a sequence of 5'-CCCTAACCCTAACCCTAACCCT-3' (its nucleotide sequence is shown in sequence 3); The cyanine dye that uses is the compound shown in formula IV:

[0072]

[0073] (1) The process of i-motif / cyanine dye fluorescence detection of silver ions is as follows:

[0074] 1) Preparation of DNA mother solution: the DNA molecule shown in Sequence 1 was dissolved in 10 mM PBS buffer solution containing 1 mM EDTA (ethylenediaminetetraacetic acid) (complexed with other metal ions to prevent interference with test results) with a pH value of 7.4 ( Disodium hydrogen acid-sodium dihydrogen phosphate buffer solution) (recipe: EDTA is dissolved in PBS buffer solution with 10 mM and pH value of 7.4 to make the final concentration 1 mM) to prepare DNA with a DNA concentration of 100 μmol / L Mother liquor, spare.

[0075] 2) Preparation of cyanine ...

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Abstract

The invention discloses a method for detecting content of silver ions through fluorescence, and discloses a method for detecting the concentration of the silver ions. The method comprises the steps of depending on characteristics that an i-motif structure can be formed through silver ion adjustment and cyanine dye aggregates can identify the i-motif structure, adding a liquid sample to be detected into a mixed solution of cyanine dye and DNA molecule capable of forming the i-motif structure, and finding out a corresponding silver ion concentration value on a standard silver ion concentration curve by measuring a fluorescence intensity value at the wavelength of 530-700nm. The method is high in specificity and high in selectivity; reagent components are simple, and a reaction process is simple; an error caused by operation can be effectively reduced, and the test precision is high. The method can realize quick detection through a common fluorescence spectrometer, and a special or extra instrument is not needed; the detection cost is low; the method is convenient for industrial popularization and application.

Description

technical field [0001] The invention belongs to the field of analysis and detection, in particular to a method for fluorescent detection of silver ion content. Background technique [0002] As a natural disinfectant, silver is widely used in the field of medicine and people's daily life. However, current research shows that silver ions can accumulate in the skin, eyes, mucous membranes, etc. of the human body, causing harm to human health. It can also combine with thiol enzymes in the body to inactivate them. At the same time, silver can also interact with various physiological metabolites such as amines, imidazoles, and carboxylic acids to cause various diseases. In recent years, with the large-scale application of nano-silver particles, silver has entered the environment in various forms, causing certain damage to the natural biological environment. Therefore, the development of fast and efficient silver ion detection methods is of great significance to human health, env...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 唐亚林史运华孙红霞杨千帆
Owner INST OF CHEM CHINESE ACAD OF SCI
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