Test paper for procalcitonin and C reactive protein as well as preparation method and detection method thereof

Abstract

The invention discloses test paper for procalcitonin and C reactive protein, which is quantum dot labeled immunity quantitative detection test paper. The test paper comprises a bottom lining and a sample mat, a filter mat, a bonding mat, a chromatographic membrane and a water absorption mat which are sequentially stuck on the bottom lining by lapping so as to form multiple layers of membrane structures, wherein the membrane structures are partially superposed; the bonding mat is coated with a quantum dot labeled procalcitonin specific antibody I, a quantum dot labeled C reactive protein specific antibody I and a quantum dot labeled rabbit IgG; and a detection line coated with a procalcitonin specific antibody II, a detection line coated with a C reactive protein specific antibody II and a quality control line coated with a goat anti-rabbit IgG antibody are formed on the chromatographic membrane. The test paper can be used for conveniently, quickly, accurately, quantitatively and simultaneously detecting the procalcitonin and the C reactive protein.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a detection test paper for procalcitonin and C-reactive protein, a preparation method and a detection method thereof. Background technique [0002] Procalcitonin (PCT) is a specific marker present in the human body. The examination of PCT plays an important role in the diagnosis of febrile disease, and it is an important marker for distinguishing bacterial and non-bacterial infections. A lower limit of PCT concentration of 0.5ng / ml by ordinary detection methods can be used to judge systemic bacterial infection, a higher concentration of 2-10ng / ml may be severe bacterial infection, and >10ng / ml may be septic shock. If the PCT concentration can be detected more accurately, local bacterial infection and intracellular pathogenic microorganism infection (such as Rickettsia, Brucella, Legionella, Mycoplasma, Chlamydia, etc.) can be judged. [0003] PCT indications: 1. Differential...

AI Technical Summary

Problems solved by technology

[0007] In the prior art, PCT and CRP cannot be detected at the same time, which brings inconvenience to clinical diagnosis and is not conducive to accurate diagnosis of etiology and disease.

In addition, the test results are greatly affected by temperature

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