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A method for extracting and purifying different forms of selenium in tobacco

A technology of tobacco and morphology, which is applied in the field of physical and chemical detection, can solve the problems of extraction and purification of selenium morphology detection that have not been reported, and achieve the effects of shortening the pretreatment cycle, improving efficiency, and high sensitivity

Active Publication Date: 2016-06-15
CHINA TOBACCO FUJIAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the speciation analysis of selenium has been deeply studied in the food field of seafood, traditional Chinese medicine, etc., and there are many reports on related sample pretreatment (extraction and purification) methods, but there is no report on the extraction and purification of selenium speciation detection in tobacco.

Method used

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  • A method for extracting and purifying different forms of selenium in tobacco
  • A method for extracting and purifying different forms of selenium in tobacco
  • A method for extracting and purifying different forms of selenium in tobacco

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The extraction and purification method established by the present invention is used to extract and purify different forms of selenium in Burley shredded tobacco.

[0038] Weigh about 0.2g of ground Burley shredded tobacco (sample 1) into a 50mL capped centrifuge tube, add 15mL aqueous solution containing 0.05mol / L hydrochloric acid, perform ultrasonic extraction at 50°C for 60min, cool to 37°C and add 10mg of For proteinase K, place on a vortex mixer and vibrate at a speed of 1500r / min for 30min. After standing still, take the supernatant and filter it through a 0.22μm aqueous phase membrane before injecting the sample.

[0039] Adopt high-performance liquid chromatography inductively coupled plasma-mass spectrometry to detect the selenium form distribution in sample 1 (working conditions are shown in Table 1), and carry out standard addition recovery, measure in parallel five times, calculate the content of various selenium forms in the sample Mean, sample recovery, an...

Embodiment 2

[0044] The extraction and purification method established by the invention is used to extract and purify selenium in different forms in flue-cured tobacco shreds.

[0045] Weigh about 0.3g of ground flue-cured tobacco shreds (sample 2) into a 50mL capped centrifuge tube, add 18mL aqueous solution containing 0.07mol / L hydrochloric acid, perform ultrasonic extraction at 65°C for 40min, cool to 37°C and add 20mg of For proteinase K, place on a vortex mixer and vibrate at a speed of 2000r / min for 20min, and after standing still, take the supernatant and filter it through a 0.22μm aqueous phase membrane before injecting the sample.

[0046] Adopt high-performance liquid chromatography inductively coupled plasma mass spectrometry (working conditions see Table 1) to detect the distribution of selenium form in sample 2, and carry out standard addition recovery, measure in parallel five times, calculate the content of various forms of selenium in the sample Mean, sample recovery, and r...

Embodiment 3

[0051] The extraction and purification method established in the present invention is used to extract and purify the different forms of selenium in the shredded tobacco.

[0052] Weigh about 0.5g of ground spice tobacco (sample 3) into a 50mL capped centrifuge tube, add 20mL aqueous solution containing 0.1mol / L hydrochloric acid, conduct ultrasonic extraction at 80°C for 30min, cool to 37°C and add 30mg of protease K, placed on a vortex mixer and vibrated at a speed of 2500r / min for 15min, after standing still, the supernatant was filtered through a 0.22μm aqueous membrane and then injected.

[0053] Adopt high-performance liquid chromatography inductively coupled plasma mass spectrometry to detect the selenium form distribution in sample 3 (working conditions are shown in Table 1), and carry out standard addition recovery, measure in parallel five times, calculate the content of various selenium forms in the sample Mean, sample recovery, and relative deviation.

[0054] The ...

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Abstract

The invention relates to a method for extracting and purifying different forms of selenium in tobacco. The method comprises the following steps: grinding a tobacco sample, extracting it with an acid solution, adding a suitable enzyme after cooling, and filtering after standing still to obtain a sample. The extraction and purification method of the present invention adopts the combination of hydrochloric acid-proteinase K extract, which ensures that the selenium in various forms is evenly extracted and distributed in the extract, which is convenient for one-time sample introduction and analysis. The method adopts vortex mixing Oscillation greatly improves the extraction efficiency and shortens the pretreatment cycle.

Description

technical field [0001] The invention belongs to the field of physical and chemical detection, and relates to a method for extracting and purifying different forms of selenium in tobacco. Background technique [0002] Tobacco contains heavy metals. During the smoking process, these heavy metals can enter the human body through the smoke, causing potential harm to the human body. Among the 107 harmful components of cigarette smoke reported in the United States in 1989, As, Cd, Cr, Pb, Ni, Cu, Hg, etc. , Hg included in the 44 harmful components of tobacco. More and more studies have confirmed that smoking has become one of the main sources of some heavy metals in the body of smokers. [0003] However, the biological toxicity of heavy metals is not only related to their content, but also determined by their existing forms to a greater extent. Taking selenium as an example, its main forms are: arsenous acid (As(Ⅲ)), arsenic acid (As(Ⅴ)), monomethylarsenic acid (MMA), dimethyla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/14
Inventor 蔡国华谢卫张建平黄朝章吴清辉黄华发邓其馨刘秀彩张鼎方
Owner CHINA TOBACCO FUJIAN IND
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