A method for producing prebiotic peptides by two-step solid-state fermentation of peanut meal

A technology of solid-state fermentation and peanut meal, which is applied in the field of animal feed and bioengineering, can solve problems such as difficulty in functioning of bacillus and neutral protease, inconsistent demand, limited content of beneficial metabolites, etc., to enhance disease prevention and resistance, The effect of stable quality and outstanding substantive features

Active Publication Date: 2016-08-03
莱阳鲁花生物蛋白有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the neutral protease produced by bacillus, under the co-fermentation of lactic acid bacteria, the fermentation system quickly becomes acidic, and it is difficult for bacillus and neutral protease to play a role, and bacillus and lactic acid bacteria are respectively aerobic microorganisms and anaerobic microorganisms. Inconsistent oxygen requirements, limited microbial growth (product Lactobacillus 10 8 CFU / g), which also leads to limited content of beneficial metabolites in the product, such as about 8% peptide content and about 3% lactic acid content

Method used

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  • A method for producing prebiotic peptides by two-step solid-state fermentation of peanut meal
  • A method for producing prebiotic peptides by two-step solid-state fermentation of peanut meal

Examples

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Embodiment 1

[0036]Use peanut meal as raw material, mix it with tap water according to the material-water ratio 1:0.8, and then sterilize at 121°C for 20 minutes. After sterilization, add 2% (v / w) of peanut meal weight into Aspergillus niger (CGMCCNo.9183) spore liquid After mixing evenly, culture at 28°C~32°C for 28h, and turn the material every 4h; after the cultivation is completed, mix 3 batches, and add 10% (v / w) Pediococcus pentosaceae (CGMCCNo.9182) according to the weight of peanut meal ), mixed evenly, and anaerobically fermented at 37°C~46°C for 42 hours, dried and pulverized at 60°C after the fermentation was completed, to obtain the probiotic peptide. The peptide content of the probiotic peptide reaches 28.4%, the lactic acid content reaches 8.8%, and the number of viable lactic acid bacteria reaches 1.2×10 9 CFU / g, acid protease reaches 228U / g.

Embodiment 2

[0038] Use peanut meal as raw material, mix it with tap water according to the material-water ratio 1:0.8, and then sterilize at 121°C for 20 minutes. After sterilization, add 2% (v / w) of peanut meal weight into Aspergillus niger (CGMCCNo.9183) spore liquid After mixing evenly, culture at 28°C~32°C for 26 hours, and turn the material every 4 hours; after the cultivation is completed, mix 3 batches, and insert 10% (v / w) Pediococcus pentosaceae (CGMCCNo.9182) according to the weight of peanut meal ), mixed evenly and anaerobically fermented at 37°C to 46°C for 36 hours, dried and pulverized at 60°C after the fermentation was completed, to obtain prebiotic peptides. The peptide content of probiotic peptides reaches 26.2%, the lactic acid content reaches 8.3%, and the number of live lactic acid bacteria reaches 1.0×10 9 CFU / g, acid protease reaches 212U / g.

Embodiment 3

[0040] Use peanut meal as raw material, mix it with tap water at a ratio of 1:1.1, and then sterilize at 120°C for 15 minutes. After sterilization, add 2% (v / w) of the weight of peanut meal to Aspergillus niger (CGMCCNo.9183) spore liquid After mixing evenly, culture at 28°C~32°C for 28h, and turn the material every 4h; after the cultivation is completed, mix 3 batches, and add 10% (v / w) Pediococcus pentosaceae (CGMCCNo.9182) according to the weight of peanut meal ), mixed evenly and anaerobically fermented at 37°C to 46°C for 36 hours, dried and pulverized at 60°C after the fermentation was completed, to obtain prebiotic peptides. The peptide content of probiotic peptides reaches 25.4%, the lactic acid content reaches 8.2%, and the number of live lactic acid bacteria reaches 1.0×10 9 CFU / g, acid protease reaches 201U / g.

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Abstract

The invention belongs to the technical field of animal feed and bioengineering, and specifically relates to a method for producing prebiotic peptides by two-step solid-state fermentation of peanut meal. Use peanut meal as raw material, mix with water and stir evenly, then sterilize, add 2% (v / w) spore liquid of Aspergillus niger (CGMCC9183) according to the weight of peanut meal after sterilization, and cultivate at 28℃~32℃ for 24~ 28h, turn the material once every 4h; after the cultivation is completed, add 10% (v / w) Pediococcus pentosaceae (CGMCC9182) according to the weight of the peanut meal, mix evenly, anaerobic fermentation at 37℃~46℃ for 36~42h, after the fermentation Dried and pulverized at 60°C to obtain prebiotic peptides. The peptide content of probiotic peptides reaches more than 25%, the lactic acid content reaches more than 8.0%, and the number of viable lactic acid bacteria reaches 10 9 More than CFU / g, acid protease reaches more than 200U / g. The invention has low production cost, easy control of the fermentation process and stable product quality.

Description

technical field [0001] The invention belongs to the technical field of animal feed and bioengineering, and specifically relates to a method for producing prebiotic peptides by two-step solid-state fermentation of peanut meal. Background technique [0002] Peanut meal is a by-product of peanut kernels that are pressed and refined for oil, and is rich in vegetable protein. Peanut meal has a high nutritional value, its metabolic energy is the highest among meal types, and its crude protein content is over 48%. It is an important plant source protein feed in my country. The annual peanut meal resource in China is about 3 million tons. However, because the protein quality of peanut meal is the same as other plant proteins, it exists in a macromolecular structure, and there are anti-nutritional factors, which hinder the digestion and absorption of protein, and the utilization rate of protein is relatively low. [0003] However, at present, the development and utilization of peanu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A23K10/12A23K10/20A23K10/18A23K20/147A23K20/189
Inventor 杜祖波陆健李秋蔡国林王珊珊朱德伟
Owner 莱阳鲁花生物蛋白有限公司
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