Detection kit for accessorily diagnosing type 2 diabetes and use method thereof

Abstract

The invention discloses a detection kit for accessorily diagnosing type 2 diabetes. The detection kit comprise a primer used for detecting the single nucleotide polymorphisms of a PDPK1 (Phosphoinositide Dependent Protein Kinase) gene, a GCK (Glucokinase) gene, a PIK3R2 (Phosphoinositide 3-Kinase Regulatory Subunit 2) gene, an MAPK9 (Mitogen Activated Protein Kinase) gene, a CBLC (Cobalamin C) gene, an MKNK2 gene, an FLOT2 gene, a TRIP10 gene and a KRAS gene, and the single nucleotide polymorphisms respectively include rs76318740, rs12702070, rs3736328, rs1363513, rs2965143, rs3810412, rs4795473, rs340141 and rs7311692. According to the detection kit, PCR (Polymerase Chain Reaction) amplification is carried out on the DNA (Deoxyribose Nucleic Acid) of a sample by virtue of the primer of the detection kit, a melting curve of a PCR amplification product is analyzed by using a fluorescent quantitative PCR instrument, then the melting curve is compared with a standard melting curve, and the Tm value of the PCR amplification product is compared, so that a corresponding genotyping result is obtained. The detection kit disclosed by the invention is capable of accessorily detecting and predicting the diabetes of testee and has the advantages of simple detection method, high detection efficiency and high pertinence, thereby promoting the early prevention of the type 2 diabetes and providing the reference for medication treatment.

Description

technical field [0001] The invention relates to the technical field of auxiliary diagnosis of diabetes, in particular to a detection kit for auxiliary diagnosis of type 2 diabetes and a use method thereof. Background technique [0002] Diabetes mellitus is a common endocrine and metabolic disease. It is caused by absolute or relative insufficiency of insulin in the blood, resulting in hyperglycemia and glycosuria, which in turn causes fat and protein metabolism disorders. Clinically, polyuria, polydipsia, polydipsia, Hyperphagia, emaciation and other manifestations, severe cases are prone to acute complications such as ketoacidosis or chronic complications such as blood vessels and nerves. The prevalence of diabetes is gradually increasing with the improvement of living conditions and the improvement of living standards. According to statistics, the number of diabetic patients in China is close to 100 million, and the prevalence rate is as high as 9.7%. Diabetes is not a s...

AI Technical Summary

Problems solved by technology

[0005] However, at present, there is no published method for detecting the rs76318740 single nucleotide polymorphism of the insulin pathway PDPK1 gene, the rs12702070 single nucleotide polymorphism of the GCK gene, the rs3736328 single nucleotide polymorphism of the PIK3R2 gene, and the MAPK9 gene. rs1363513 SNP, CBLC gene rs2965143 SNP, MKNK2 gene rs3810412 SNP, FLOT2 gene rs4795473 SNP, TRIP10 gene rs340141 SNP Relevant research reports on kits for the degree of correlation between morphism, KRAS gene rs7311692 single nucleotide polymorphism and type 2 diabetes

Images