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Method for determining immune activity of tracheitis vaccine by using trace hemolysis spectrophotometry

A spectrophotometry and immune activity technology, which is applied in the field of measuring the immune activity of bronchitis vaccine by micro-hemolysis spectrophotometry, can solve the problems of poor objectivity and repeatability, and the influence of subjective factors is large, and achieve the effect of good repeatability.

Inactive Publication Date: 2014-12-10
蒋建江
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Problems solved by technology

[0004] Since there is no activity detection method in the finished product standard of tracheitis vaccine at present, there are bacterial lysates (Fanflux) in the domestic biological products whose main function is to prevent repeated respiratory infections and chronic bronchitis, and the activity detection of this product is "hemolytic plaque "Method", because this method is through visual observation and manual counting, the subjective factors are greatly affected, and the objectivity and repeatability are relatively poor

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  • Method for determining immune activity of tracheitis vaccine by using trace hemolysis spectrophotometry
  • Method for determining immune activity of tracheitis vaccine by using trace hemolysis spectrophotometry

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Embodiment Construction

[0025] The present invention will now be further described in conjunction with specific examples, and the following examples are intended to illustrate the present invention rather than further limit the present invention.

[0026] A method for measuring the immune activity of tracheitis vaccine by micro-hemolysis spectrophotometry, comprising the following process steps:

[0027] (1) Test preparation:

[0028] a. Test product: Preparation of tracheitis vaccine: culture and collect type A hemolytic streptococcus, staphylococcus albus, and Neisserial diplococcus, sterilize them with formaldehyde, and mix them with equal concentrations of the three bacteria after centrifugation and washing , diluted with PBS;

[0029] b Test subjects: 50 male BABL / C mice, 5-6 weeks old, weighing 18-22g, and 5 guinea pigs weighing 250-300g, among them, the mice were divided into 5 groups, 10 in each group mice, specifically:

[0030] The first group: absolute blank control group;

[0031] The...

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Abstract

The invention relates to the technical field of determination of immunity activity of tracheitis vaccines, and particularly relates to a method for determining immune activity of a tracheitis vaccine by using trace hemolysis spectrophotometry. The method comprises the following processing steps: preparing for a test; immunizing; preparing spleen cell suspension; preparing SRBC suspension; preparing complement in blood serum; detecting antibody-forming cells; carrying out statistical analysis; and immunizing tracheitis vaccines of different concentrations. According to the method disclosed by the invention, two methods including hemolytic plaque formation test and antibody-forming cell determination by spectrophotometry are optimized and innovated, the trace hemolysis spectrophotometry is built to determine the antibody-forming cell, reaction is carried out in a 96 hole cell culture plate, a large number of samples can be detected at one time by virtue of a microplate reader, and a corresponding result can be obtained by repeating for a plurality of times, therefore, the method has the advantages of being good in scientificity, accuracy and repeatability.

Description

technical field [0001] The invention relates to the technical field of measuring the immune activity of the tracheitis vaccine, in particular to a method for measuring the immune activity of the tracheitis vaccine by micro-hemolysis spectrophotometry. Background technique [0002] Immunological activity is to study the relationship between the biochemical and physiological effects and mechanisms of drugs, as well as the dose and effect. The purpose is to determine the curative effect of drugs for clinical prevention, diagnosis and treatment purposes, to determine the strength of drugs, and to clarify the role of drugs. and mechanism, discovering a wide range of pharmacological effects that are expected to be used beyond the clinic. [0003] Hemolytic plaque formation test (PFC) and spectrophotometric method to measure antibody-forming cells (QHS). The former is that antibody-forming cells release hemolysin, which dissolves sheep red blood cells with the help of complement, f...

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Application Information

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IPC IPC(8): G01N33/15G01N21/31
Inventor 蒋建江潘翔
Owner 蒋建江