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Method for preparing triple inactivated vaccine

An inactivated vaccine and inactivated technology, applied in the field of vaccines, can solve the problems of high cost, mixed infection, and high stress in pigs

Inactive Publication Date: 2014-12-17
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the vaccines used on the market are only single or dual inactivated vaccines or live vaccines for the prevention of Haemophilus parasuis, Streptococcus suis or Mycoplasma pneumonia, and one or more of these three diseases Usually co-infected with swine fever, porcine blue-ear disease, porcine circus disease, etc., or mixed infection of these three diseases, commercially available commercial vaccines need to be immunized multiple times, the cost is high, and the stress on pigs is greater
In addition, the inactivated vaccine for simultaneously preventing Haemophilus parasuis, Streptococcus suis and Mycoplasma pneumonia has not been reported at home and abroad.

Method used

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  • Method for preparing triple inactivated vaccine
  • Method for preparing triple inactivated vaccine
  • Method for preparing triple inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 1

[0021] Embodiment 1 A kind of preparation method of triple inactivated vaccine

[0022] A kind of preparation method of haemophilus parasuis disease, streptococcus suis, mycoplasma swine pneumonia triple inactivated vaccine, its steps are as follows:

[0023] 1 Production strain preparation

[0024] 1.1 First-class seed propagation

[0025] Streak-inoculate the freeze-dried strains of Haemophilus parasuis type 4, Haemophilus parasuis type 5, and Streptococcus suis type 2 on TSA / NAD plates, culture at 37°C for 18-24 hours, and pick a single colony Inoculate in TSB / NAD liquid medium, culture at 37°C for 12-16 hours, it is a first-class seed; freeze-dried strains of Mycoplasma hyopneumoniae are streaked and inoculated on solid medium, cultured at 37°C for 7 days, pick a single colony for inoculation On the slant solid medium, cultured at 37°C for 7 days, it is the first-class seed.

[0026] 1.2 Secondary seed propagation

[0027]The first-grade seeds of Haemophilus parasuis t...

Embodiment 2

[0051] Embodiment 2: The safety research of the vaccine prepared in embodiment 1

[0052] Get the vaccine prepared in Example 1 and inject intramuscularly into healthy susceptible weaned piglets at the age of 4 to 5 weeks, 2 mL per head (including 1 head), inject 5 heads of each vaccine, and 5 heads of the control group. After 2 weeks, use the same Methods and the same dose of the second immunization, observed for 2 weeks, there should be no local or systemic adverse reactions and all healthy and alive. The results are shown in Table 1.

[0053] Table 1 Safety study results

[0054]

[0055] Safety test results show: Haemophilus parasuis, Streptococcus suis and Mycoplasma pneumonia triple white oil adjuvant inactivated vaccine, Haemophilus parasuis, Streptococcus suis and Mycoplasma pneumonia triple levamisole white oil Adjuvanted Inactivated Vaccine, Haemophilus Parasuis, Streptococcus Suis and Mycoplasma Pneumonia Triple Scutellaria Polysaccharide White Oil Adjuvanted I...

Embodiment 3

[0056] Example 3: Vaccine Efficacy Study

[0057] 1 Experimental design

[0058] 1.1 Vaccine Immunization

[0059] Get the vaccine prepared in Example 1, inject various vaccines into 4-5 weeks old healthy susceptible weaned piglets, 2mL per head (one head portion), 20 pigs in each group, the second immunization after the first immunization 3 weeks, the same immunization dose, At the same time, 20 pigs in each vaccine group were used as controls, which were not immunized.

[0060] Purchase commercially available Haemophilus parasuis inactivated vaccines (types 4 and 5), purchased from China Animal Husbandry Co., Ltd., batch number 1209006; Streptococcus suis inactivated vaccines (type 2), purchased from Guangdong Yongshun Biological Products Co., Ltd., batch number 2012010; inactivated vaccine for mycoplasma swine pneumonia (strain J), purchased from Spain's Hyperion Company, batch number 31KB-1. The vaccines purchased above were injected into 4-5 week-old healthy susceptibl...

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Abstract

The invention provides a method for preparing a triple inactivated vaccine. The triple inactivated vaccine has a relatively good immunizing effect on haemophilus parasuis, swine streptococcosis and mycoplasma pneumoniae of swine, and the aims of preventing multiple diseases by one injection, reducing cost and reducing swine stress.

Description

technical field [0001] The invention relates to the technical field of vaccines, in particular to a method for preparing a triple inactivated vaccine. Background technique [0002] Haemophilus parasuis, Streptococcus suis and Mycoplasma hyopneumoniae are important pathogens that endanger the pig industry today, and are often used as concurrent or secondary infection pathogens of swine fever, porcine reproductive and respiratory syndrome, porcine circovirus disease and swine influenza , Seriously endanger the development of my country and even the world's pig industry. [0003] Haemophilus Parasuis of Swine (HPS) is the pathogen of Haemophilus parasuis disease, a commensal bacterium in the upper respiratory tract of pigs, which can invade the body under certain conditions and cause fibrinous polymenorritis A systemic disease characterized by , arthritis, meningitis and high fever. Haemophilus parasuis disease, also known as Glsser's Disease, can affect pigs aged 2 weeks to 4...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61P31/04A61K39/09A61K39/02A61K39/102
Inventor 厚华艳吕茂杰杨保收盛长忠梁武
Owner TIANJIN RINGPU BIO TECH
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